##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630816.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1035944
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.869032495965033	33.0	31.0	34.0	30.0	34.0
2	31.97193670700347	33.0	31.0	34.0	30.0	34.0
3	32.03061362390245	33.0	31.0	34.0	30.0	34.0
4	35.68160634165553	37.0	35.0	37.0	33.0	37.0
5	35.70315480373456	37.0	35.0	37.0	35.0	37.0
6	35.78429142888032	37.0	35.0	37.0	35.0	37.0
7	35.751947016441044	37.0	35.0	37.0	35.0	37.0
8	35.777482180503966	37.0	35.0	37.0	35.0	37.0
9	37.427853243032445	39.0	37.0	39.0	34.0	39.0
10	37.35728282609871	39.0	37.0	39.0	34.0	39.0
11	37.501627501100444	39.0	37.0	39.0	35.0	39.0
12	37.43111307174905	39.0	37.0	39.0	34.0	39.0
13	37.493652166526374	39.0	37.0	39.0	35.0	39.0
14	38.59364791919254	40.0	38.0	41.0	34.0	41.0
15	38.56378916234854	40.0	38.0	41.0	34.0	41.0
16	38.5025368166619	40.0	38.0	41.0	34.0	41.0
17	38.606916010904065	40.0	38.0	41.0	34.0	41.0
18	38.65656541280224	40.0	38.0	41.0	34.0	41.0
19	38.73183106422741	40.0	38.0	41.0	34.0	41.0
20	38.725707181083145	40.0	38.0	41.0	35.0	41.0
21	38.65056412315724	40.0	38.0	41.0	34.0	41.0
22	38.553850401179986	40.0	38.0	41.0	34.0	41.0
23	38.48788544554532	40.0	38.0	41.0	34.0	41.0
24	38.40369170534315	40.0	38.0	41.0	34.0	41.0
25	38.333637725591345	40.0	38.0	41.0	34.0	41.0
26	38.0610554238453	40.0	37.0	41.0	33.0	41.0
27	37.86318082830732	40.0	37.0	41.0	33.0	41.0
28	37.73272493493857	40.0	36.0	41.0	33.0	41.0
29	37.5656724687821	40.0	36.0	41.0	33.0	41.0
30	37.35739480126339	39.0	36.0	41.0	33.0	41.0
31	37.1513633941603	39.0	35.0	41.0	32.0	41.0
32	36.91391716154541	39.0	35.0	41.0	32.0	41.0
33	36.74193778814299	39.0	35.0	41.0	31.0	41.0
34	36.54451881568888	39.0	35.0	41.0	31.0	41.0
35	36.34232448858239	39.0	35.0	41.0	30.0	41.0
36	36.14879182658522	39.0	35.0	41.0	30.0	41.0
37	35.80543735954839	38.0	35.0	41.0	29.0	41.0
38	35.541695303993265	38.0	35.0	40.0	27.0	41.0
39	35.298512274794774	38.0	35.0	40.0	25.0	41.0
40	35.00125006757122	38.0	35.0	40.0	24.0	41.0
41	34.69140416856509	38.0	34.0	40.0	22.0	41.0
42	34.365373997050035	38.0	34.0	40.0	20.0	41.0
43	33.504125705636596	37.0	33.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	15.0
10	16.0
11	14.0
12	12.0
13	8.0
14	6.0
15	4.0
16	9.0
17	26.0
18	58.0
19	109.0
20	240.0
21	474.0
22	892.0
23	1606.0
24	2485.0
25	4079.0
26	6028.0
27	8696.0
28	12473.0
29	17283.0
30	22095.0
31	27512.0
32	33611.0
33	42145.0
34	54002.0
35	68158.0
36	93775.0
37	156426.0
38	209559.0
39	274127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.38629115087302	20.083807618944654	13.356320418864339	20.173580811317986
2	18.86771871838632	22.770921980338706	34.475608720162484	23.885750581112493
3	22.296282424532603	26.466681596688623	29.566366521742488	21.670669457036286
4	13.351011251573444	16.227325029152155	32.74057284949766	37.681090869776746
5	12.455016873498954	41.24209416725228	31.616091217285874	14.686797741962888
6	31.521491509193545	33.64708903183956	17.25073942220815	17.580680036758743
7	26.33009120184103	33.59264593452928	21.171125080120163	18.906137783509532
8	30.905145451877708	30.369498737383488	19.778482234560943	18.946873576177865
9	28.211853150363343	11.65893137080769	22.63182179731723	37.497393681511745
10	19.446417953093988	29.395604395604398	30.055678685334343	21.10229896596727
11	32.005880626752024	23.05076336172612	20.488269636196552	24.45508637532531
12	21.848767887067254	30.862865174179298	27.568285544392364	19.720081394361085
13	30.91248175577058	20.13998826191377	27.993211988292803	20.95431799402284
14	22.680183484821573	22.888013251681556	29.621871452510945	24.809931810985923
15	26.650668375896768	26.677214212351245	26.84894164163314	19.823175770118848
16	22.46926474790143	26.013085649417345	28.66380808229016	22.853841520391065
17	20.643779972662614	28.274501324395914	28.70618489030295	22.37553381263852
18	20.03361185546709	23.732750032820306	32.93874958491965	23.294888526792953
19	20.769076320727763	26.041175970901904	33.637918651973465	19.551829056396873
20	22.25564316217865	23.705914605422688	34.57831697466272	19.46012525773594
21	23.0720000308897	24.81118670507286	32.49596503285892	19.62084823117852
22	22.92566007428973	25.284860957735166	32.23726379032071	19.552215177654393
23	21.33262029607778	25.19682531102067	32.42800769153545	21.042546701366096
24	20.21933618033407	26.880024402863473	32.51218212567475	20.388457291127708
25	20.000695018263535	26.6860950012742	32.837103163877586	20.47610681658468
26	20.656908095418284	27.34201848748581	32.17123705528484	19.82983636181106
27	20.3692477585661	27.266917902898225	32.45851127087951	19.905323067656166
28	19.44661101372275	27.141428494204323	33.095997467044555	20.31596302502838
29	19.282026827704975	27.47986377642035	33.60007876873654	19.638030627138146
30	18.435166379649864	28.545461916860372	33.596507147104475	19.422864556385285
31	20.202926026889482	28.64093039778212	31.495042203053448	19.66110137227495
32	18.144127481794385	28.24978956391465	32.71383395241442	20.89224900187655
33	18.260929162194095	28.13240870162866	33.00921671441699	20.59744542176025
34	19.60685133559343	27.43961063532392	32.97726518035724	19.97627284872541
35	18.351667657711225	28.649618126076316	32.88266547226491	20.116048743947548
36	18.862506081409805	28.960445738379683	31.574486651788124	20.602561528422385
37	18.740395233719198	28.044469585228544	32.28041284084854	20.934722340203717
38	18.73663055145838	27.417119072073394	32.099804622643695	21.74644575382453
39	18.704582487084245	27.830075756990723	32.675414887291204	20.789926868633827
40	17.709741067084707	28.006629701991614	32.85370637795093	21.429922852972748
41	18.036785772203903	27.694643725915686	32.632458897392134	21.636111604488274
42	17.594001220143173	28.4005699149761	33.09281196667001	20.912616898210715
43	17.107005784096437	27.289892117720647	32.52337964214282	23.079722456040095
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	355.0
1	402.0
2	449.0
3	1644.0
4	2839.0
5	2839.0
6	4066.0
7	5293.0
8	5493.5
9	5694.0
10	8174.0
11	10654.0
12	10654.0
13	18113.5
14	25573.0
15	35868.0
16	46163.0
17	41331.5
18	36500.0
19	36500.0
20	38183.5
21	39867.0
22	29360.5
23	18854.0
24	18148.0
25	17442.0
26	17442.0
27	18083.5
28	18725.0
29	20701.5
30	22678.0
31	24953.0
32	27228.0
33	27228.0
34	30646.5
35	34065.0
36	36781.5
37	39498.0
38	44032.0
39	48566.0
40	48566.0
41	51140.0
42	53714.0
43	56125.5
44	58537.0
45	59072.5
46	59608.0
47	59608.0
48	61998.0
49	64388.0
50	64149.5
51	63911.0
52	61844.5
53	59778.0
54	59778.0
55	58947.5
56	58117.0
57	54548.5
58	50980.0
59	47611.0
60	44242.0
61	44242.0
62	39635.0
63	35028.0
64	30989.5
65	26951.0
66	24463.0
67	21975.0
68	21975.0
69	17779.5
70	13584.0
71	12060.0
72	10536.0
73	8142.5
74	5749.0
75	5749.0
76	4500.5
77	3252.0
78	2436.5
79	1621.0
80	1322.5
81	1024.0
82	1024.0
83	963.0
84	902.0
85	841.0
86	780.0
87	610.5
88	441.0
89	441.0
90	380.0
91	319.0
92	186.5
93	54.0
94	29.5
95	5.0
96	5.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1035944.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.262465090463984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.90861708048345	37.526483863040276
2	9.701746552463517	8.782499292948184
3	2.6935760872081316	3.657536808472999
4	1.1717091793707333	2.121377833097761
5	0.6274842532981342	1.4200742054861328
6	0.44019842530936043	1.1954679515065287
7	0.3085551428800974	0.9776176468165474
8	0.23804021408401418	0.8619429504083411
9	0.19185947490364325	0.7815629506590812
>10	1.4041861462528444	12.730741588912542
>50	0.16758239667405414	5.323720156864742
>100	0.13064776795155758	11.909880803339766
>500	0.010887313988433211	3.429795218539434
>1k	0.004056058152553549	2.7737125744701654
>5k	2.1347674487123944E-4	0.9457639057658321
>10k+	6.404302346137183E-4	5.5618222496717475
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21470	2.0725058497370514	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20069	1.9372668792907726	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16022	1.5466086969952044	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9788	0.9448387171507341	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2487	0.24007089186288064	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2439	0.23543743677264406	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	2439	0.23543743677264406	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2006	0.1936398106461353	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1783	0.17211355053941138	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	1561	0.1506838207470674	RNA PCR Primer, Index 17 (95% over 21bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1495	0.14431281999799217	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1456	0.14054813773717498	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1344	0.12973674252662307	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1281	0.1236553327206876	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1250	0.12066289297490984	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1249	0.12056636266052992	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1203	0.11612596819905324	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1200	0.11583637725591345	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	1163	0.11226475562385611	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	1154	0.11139598279443677	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1119	0.10801742179113928	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	1058	0.10212907261396369	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.791818862795672E-4	0.0
3	0.0	0.0	0.0	0.001447954715698918	0.0
4	0.0	0.0	0.0	0.0026063184882580527	0.0
5	0.0	0.0	0.0	0.003088970060157692	0.0
6	0.0	0.0	0.0	0.0031855003745376196	0.0
7	0.0	0.0	0.0	0.0039577428895770424	0.0
8	0.0	0.0	0.0	0.004150803518336898	0.0
9	0.0	0.0	0.0	0.006564061377835095	0.0
10	0.0	0.0	0.0	0.010328743638652282	0.0
11	0.0	0.0	0.0	0.012645471183770551	0.0
12	0.0	0.0	0.0	0.015058729043268748	0.0
13	0.0	0.0	0.0	0.015541380615168387	0.0
14	0.0	0.0	0.0	0.017278926274007088	0.0
15	0.0	0.0	0.0	0.01998177507664507	0.0
16	0.0	0.0	0.0	0.027607669912659373	0.0
17	0.0	0.0	0.0	0.03996355015329014	0.0
18	0.0	0.0	0.0	0.04575536901608581	0.0
19	0.0	0.0	0.0	0.05482921856779903	0.0
20	0.0	0.0	0.0	0.05984879491555528	0.0
21	0.0	0.0	0.0	0.07326650861436526	0.0
22	0.0	0.0	0.0	0.10473539110222174	0.0
23	0.0	0.0	0.0	0.15695829118176272	0.0
24	0.0	0.0	0.0	0.23814028557528205	0.0
25	0.0	0.0	0.0	0.25261983273227123	0.0
26	0.0	0.0	0.0	0.27935872981551124	0.0
27	0.0	0.0	0.0	0.30773864224321007	0.0
28	0.0	0.0	0.0	0.36758743715876535	0.0
29	0.0	0.0	0.0	0.46189755430795487	0.0
30	0.0	0.0	0.0	0.6240684824662337	0.0
31	0.0	0.0	0.0	1.1225510259241813	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTAC	45	1.3230887E-4	24.666668	3
GCCGTCT	170	0.0	23.941177	36
TCTACAC	60	3.7258305E-5	21.583332	3
ACACCGC	45	0.0038255563	20.555557	6
TAGCACG	45	0.0038255563	20.555557	4
CGAATGT	45	0.0038255563	20.555557	4
CCGTCTT	200	0.0	20.35	37
CAGTCGG	295	0.0	20.067797	10
GGTAAAC	130	3.274181E-11	19.923075	35
GCAGTCG	310	0.0	19.693548	9
TCTATAC	105	2.258821E-8	19.380953	3
CTATACA	50	0.0070344238	18.5	4
GGTATTG	50	0.0070344238	18.5	33
ACTGTGC	60	9.235979E-4	18.5	8
GCAGCGT	60	9.235979E-4	18.5	1
CGTTTGG	60	9.235979E-4	18.5	28
AGTCGGT	315	0.0	18.206348	11
GGTATCA	7960	0.0	18.197865	1
TCCTCGC	235	0.0	18.106382	21
GGAACGT	95	3.60569E-6	17.526316	8
>>END_MODULE