FastQCFastQC Report
Fri 10 Feb 2017
ERR1630815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063801
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46220.4344797570222251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44290.4163372660864203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36630.34433131760545443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.2369804126899674No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC15950.14993405721558825No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT14300.13442363750363084No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA13230.12436536532678576No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA12410.11665715674266146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT502.7019184E-422.26
CGGATCC1351.8189894E-1220.55555532
TACACCG555.142747E-420.1818185
CGAACGA902.1519081E-618.516
GGTATCA22400.018.2522341
GCCGGAT1603.45608E-1117.3437530
GCGTTAT1401.8681021E-917.1785721
CGTTATT1452.9776857E-916.5862082
GCGCAAG2200.015.9772731
GTATTAG2800.015.8571431
ATAATAC1302.5882218E-715.6538463
ATTAGAT957.058883E-515.5789483
ACGAACG957.058883E-515.57894815
ACGGACC2055.456968E-1215.3414648
TAGGGAG1455.3432814E-815.3103465
GTACCCT855.3636834E-415.2352944
GGATCCC1701.4842954E-915.23529433
CTAGCGG855.3636834E-415.23529429
CGGACCA2109.094947E-1214.97619159
GTGCCCG750.00410413814.89
Produced by FastQC (version 0.11.5)