##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630815.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1063801
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477159731942347	31.0	31.0	34.0	30.0	34.0
2	31.639303779560276	31.0	31.0	34.0	30.0	34.0
3	31.741256118390563	31.0	31.0	34.0	30.0	34.0
4	35.43779616676427	37.0	35.0	37.0	33.0	37.0
5	35.353144996103595	37.0	35.0	37.0	33.0	37.0
6	35.390180118274	37.0	35.0	37.0	33.0	37.0
7	35.372376036495545	37.0	35.0	37.0	33.0	37.0
8	35.364595445952766	37.0	35.0	37.0	33.0	37.0
9	36.99571254398144	39.0	37.0	39.0	33.0	39.0
10	36.8299362380746	39.0	37.0	39.0	33.0	39.0
11	36.97785770082938	39.0	37.0	39.0	33.0	39.0
12	36.88851110311045	39.0	37.0	39.0	33.0	39.0
13	36.96557250839208	39.0	37.0	39.0	33.0	39.0
14	38.09233963871063	40.0	37.0	41.0	33.0	41.0
15	38.04362658053527	40.0	37.0	41.0	33.0	41.0
16	38.019905978655785	40.0	37.0	41.0	33.0	41.0
17	38.00129347500143	40.0	37.0	41.0	33.0	41.0
18	37.986785122405415	40.0	37.0	41.0	33.0	41.0
19	38.00821676234559	40.0	37.0	41.0	33.0	41.0
20	37.98716959280918	40.0	37.0	41.0	33.0	41.0
21	37.90057256949373	40.0	37.0	41.0	33.0	41.0
22	37.83195729276434	40.0	37.0	41.0	33.0	41.0
23	37.80349896268193	40.0	37.0	41.0	33.0	41.0
24	37.741051192845276	39.0	37.0	41.0	32.0	41.0
25	37.69029827947144	39.0	37.0	41.0	32.0	41.0
26	37.4418824573393	39.0	37.0	41.0	32.0	41.0
27	37.29994425649158	39.0	36.0	40.0	32.0	41.0
28	37.18515587031785	39.0	36.0	40.0	31.0	41.0
29	37.07361057190207	39.0	36.0	40.0	31.0	41.0
30	36.97783608024433	39.0	36.0	40.0	31.0	41.0
31	36.91054717940668	39.0	36.0	40.0	31.0	41.0
32	36.819278229668896	39.0	35.0	40.0	30.0	41.0
33	36.765945886495686	39.0	35.0	40.0	30.0	41.0
34	36.684706068146205	39.0	35.0	40.0	30.0	41.0
35	36.61336753772557	39.0	35.0	40.0	30.0	41.0
36	36.57251215217884	39.0	35.0	40.0	30.0	41.0
37	36.35179887967768	38.0	35.0	40.0	30.0	41.0
38	36.22216091167427	38.0	35.0	40.0	30.0	41.0
39	36.13925724830114	38.0	35.0	40.0	29.0	41.0
40	36.011579233334054	38.0	35.0	40.0	29.0	41.0
41	35.8703573318694	38.0	35.0	40.0	28.0	41.0
42	35.764667451901246	38.0	35.0	40.0	28.0	41.0
43	34.87447652333472	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	0.0
11	2.0
12	1.0
13	0.0
14	2.0
15	2.0
16	10.0
17	32.0
18	62.0
19	155.0
20	283.0
21	499.0
22	964.0
23	1678.0
24	2616.0
25	4074.0
26	6274.0
27	8922.0
28	12439.0
29	17110.0
30	22429.0
31	28446.0
32	36431.0
33	46450.0
34	59832.0
35	78876.0
36	110783.0
37	169162.0
38	275702.0
39	180562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27209694294328	20.024327858311846	12.523488885609243	24.180086313135636
2	19.19917352963571	21.68215671916082	33.47834792409483	25.640321827108643
3	21.289884104263862	24.02817820250216	28.93670902734628	25.745228665887698
4	14.899215172762576	15.641741265518643	34.391018620963884	35.0680249407549
5	14.305495106697588	37.43482098625589	33.98389360416093	14.275790302885596
6	35.03550006063164	34.757346533797204	15.336703011183484	14.870450394387671
7	28.90033004293096	30.61766251394763	21.611184798660652	18.87082264446076
8	28.33189666112365	32.19323914905137	20.066911010611946	19.40795317921303
9	26.84628046034926	13.773346706761885	20.44273318035986	38.937639652529
10	17.656685789917475	27.008152840615868	32.39656665109358	22.93859471837308
11	35.30368931783294	22.17106394899046	21.38435666069124	21.14089007248536
12	22.583735115872237	26.039644632783766	28.413584871606623	22.963035379737377
13	30.309804183301196	19.993683029062765	25.47459534254997	24.221917445086063
14	22.959369280532734	21.089752688707755	25.664292475754397	30.286585555005118
15	25.741844574314182	28.01830417531098	22.76798010154155	23.471871148833287
16	24.871475022114097	26.464818137978813	24.629324469520146	24.034382370386943
17	23.60535476089983	26.58448337612016	25.62800749388278	24.182154369097226
18	23.198323746640582	24.938028823059952	27.803602365480014	24.060045064819455
19	24.41133257065936	25.284804206801837	27.716462007461924	22.58740121507688
20	25.02610920651513	24.62575237285921	26.835376165279033	23.51276225534663
21	25.00693268759853	24.99292630858591	26.817045669255812	23.183095334559752
22	24.808869328004015	25.058916094269513	27.08448290610744	23.047731671619033
23	24.0488587621181	24.976757871067992	26.85351865621484	24.12086471059907
24	23.720977889661693	25.74833074983009	27.092943135041235	23.437748225466983
25	24.013325800596164	25.632237608349683	26.643892983744138	23.710543607310015
26	23.802290089969837	26.15592577935159	26.70161054558136	23.34017358509721
27	24.187418511544923	25.027237237039635	26.905690067973236	23.879654183442202
28	23.433236103368955	25.63016955238809	27.083166870495518	23.85342747374744
29	23.58279415040971	25.988037236287614	27.372506700031302	23.056661913271373
30	23.68262485182849	26.058163133894404	26.763464219341778	23.495747794935333
31	23.203305881457155	26.52526177358359	26.83706821106579	23.434364133893464
32	23.104885218194006	25.69540731772202	26.728119262907253	24.471588201176726
33	22.50599501222503	26.22163355740406	27.288750433586735	23.983620996784172
34	24.2127051958026	25.087680872644412	27.01520303139403	23.68441090015896
35	23.52009445375592	25.338291654172163	27.289126443761567	23.85248744831035
36	22.74767555210044	26.303979785693	27.183373582089132	23.764971080117427
37	23.474315214969717	25.231974777237472	27.57912429110332	23.71458571668949
38	23.303700598138185	25.252091321591163	27.38087292642139	24.063335153849263
39	23.2783199113368	25.045567733062857	27.634679794435236	24.041432561165106
40	22.553560299341697	25.027237237039635	28.159307990874233	24.259894472744435
41	21.742224344590767	25.39394116004779	28.561074862685786	24.30275963267566
42	22.41340250667183	25.242127051958025	28.76637641814587	23.57809402322427
43	22.568506703791407	24.799281068545714	28.37861592534694	24.25359630231594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	390.0
1	317.0
2	244.0
3	510.5
4	777.0
5	777.0
6	1097.5
7	1418.0
8	1400.5
9	1383.0
10	2017.5
11	2652.0
12	2652.0
13	4633.0
14	6614.0
15	9051.5
16	11489.0
17	11234.0
18	10979.0
19	10979.0
20	12692.5
21	14406.0
22	13781.5
23	13157.0
24	14883.0
25	16609.0
26	16609.0
27	18671.5
28	20734.0
29	23578.0
30	26422.0
31	29774.0
32	33126.0
33	33126.0
34	36778.5
35	40431.0
36	44293.0
37	48155.0
38	51203.0
39	54251.0
40	54251.0
41	56128.0
42	58005.0
43	58936.0
44	59867.0
45	61490.5
46	63114.0
47	63114.0
48	66488.5
49	69863.0
50	69277.5
51	68692.0
52	68854.5
53	69017.0
54	69017.0
55	66690.0
56	64363.0
57	63152.0
58	61941.0
59	62094.0
60	62247.0
61	62247.0
62	57037.5
63	51828.0
64	47200.0
65	42572.0
66	36988.0
67	31404.0
68	31404.0
69	27752.0
70	24100.0
71	20280.0
72	16460.0
73	12106.5
74	7753.0
75	7753.0
76	5675.5
77	3598.0
78	2854.0
79	2110.0
80	1780.0
81	1450.0
82	1450.0
83	1124.0
84	798.0
85	708.0
86	618.0
87	539.0
88	460.0
89	460.0
90	359.0
91	258.0
92	146.0
93	34.0
94	22.0
95	10.0
96	10.0
97	5.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1063801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.799420305264356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.30464647306306	56.082800290749304
2	6.735745784654412	8.460018611998501
3	1.5167282660442145	2.8574896760455633
4	0.626701881618857	1.574260594795305
5	0.34554757638583206	1.0850093742459654
6	0.22465386072826024	0.8464879333844603
7	0.16753636792349547	0.7364830749951501
8	0.12516910463540423	0.6288437768985891
9	0.0958273738915564	0.541611317678901
>10	0.6665369951781771	8.357651670726199
>50	0.09584100158038733	4.185686432091221
>100	0.08936739381458575	10.79467251783528
>500	0.004348412999300472	1.7912021408338796
>1k	0.0013495074825415256	2.0577825877217313
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4622	0.4344797570222251	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4429	0.4163372660864203	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3663	0.34433131760545443	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2521	0.2369804126899674	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1595	0.14993405721558825	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1430	0.13442363750363084	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1323	0.12436536532678576	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1241	0.11665715674266146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	9.400254370883276E-5	0.0	0.0	5.640152622529965E-4	0.0
3	9.400254370883276E-5	0.0	0.0	0.001504040699341324	0.0
4	9.400254370883276E-5	0.0	0.0	0.002632071223847317	0.0
5	3.76010174835331E-4	0.0	0.0	0.003102083942391481	0.0
6	3.76010174835331E-4	0.0	0.0	0.003102083942391481	0.0
7	3.76010174835331E-4	0.0	0.0	0.003196086486100314	0.0
8	3.76010174835331E-4	0.0	0.0	0.0033840915735179793	0.0
9	3.76010174835331E-4	0.0	0.0	0.0054521475351123	0.0
10	4.700127185441638E-4	0.0	0.0	0.00714419332187129	0.0
11	5.640152622529965E-4	0.0	0.0	0.007896213671541951	0.0
12	5.640152622529965E-4	0.0	0.0	0.00883623910863028	0.0
13	5.640152622529965E-4	0.0	0.0	0.009306251827174443	0.0
14	5.640152622529965E-4	0.0	0.0	0.010152274720553939	0.0
15	6.580178059618293E-4	0.0	0.0	0.011374307788768764	0.0
16	6.580178059618293E-4	0.0	0.0	0.01297235103181892	0.0
17	6.580178059618293E-4	0.0	0.0	0.015792427343083903	0.0
18	6.580178059618293E-4	9.400254370883276E-5	0.0	0.01729646804242523	0.0
19	6.580178059618293E-4	9.400254370883276E-5	0.0	0.018894511285475386	0.0
20	6.580178059618293E-4	9.400254370883276E-5	0.0	0.0215265825093227	9.400254370883276E-5
21	6.580178059618293E-4	9.400254370883276E-5	0.0	0.025662694432511342	9.400254370883276E-5
22	7.52020349670662E-4	9.400254370883276E-5	0.0	0.032148869948420804	9.400254370883276E-5
23	7.52020349670662E-4	9.400254370883276E-5	0.0	0.042113139581557074	9.400254370883276E-5
24	7.52020349670662E-4	9.400254370883276E-5	0.0	0.05677753640013499	1.880050874176655E-4
25	7.52020349670662E-4	9.400254370883276E-5	0.0	0.06100765086703246	1.880050874176655E-4
26	8.460228933794948E-4	9.400254370883276E-5	0.0	0.06843385182003026	1.880050874176655E-4
27	9.400254370883276E-4	9.400254370883276E-5	0.0	0.08018416978363435	1.880050874176655E-4
28	0.0010340279807971604	9.400254370883276E-5	0.0	0.11035898631416965	1.880050874176655E-4
29	0.0010340279807971604	9.400254370883276E-5	0.0	0.1560442225566624	1.880050874176655E-4
30	0.0010340279807971604	9.400254370883276E-5	0.0	0.219777947191251	1.880050874176655E-4
31	0.0010340279807971604	9.400254370883276E-5	0.0	0.44519604700503196	1.880050874176655E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACCGT	50	2.7019184E-4	22.2	6
CGGATCC	135	1.8189894E-12	20.555555	32
TACACCG	55	5.142747E-4	20.181818	5
CGAACGA	90	2.1519081E-6	18.5	16
GGTATCA	2240	0.0	18.252234	1
GCCGGAT	160	3.45608E-11	17.34375	30
GCGTTAT	140	1.8681021E-9	17.178572	1
CGTTATT	145	2.9776857E-9	16.586208	2
GCGCAAG	220	0.0	15.977273	1
GTATTAG	280	0.0	15.857143	1
ATAATAC	130	2.5882218E-7	15.653846	3
ATTAGAT	95	7.058883E-5	15.578948	3
ACGAACG	95	7.058883E-5	15.578948	15
ACGGACC	205	5.456968E-12	15.341464	8
TAGGGAG	145	5.3432814E-8	15.310346	5
GTACCCT	85	5.3636834E-4	15.235294	4
GGATCCC	170	1.4842954E-9	15.235294	33
CTAGCGG	85	5.3636834E-4	15.235294	29
CGGACCA	210	9.094947E-12	14.9761915	9
GTGCCCG	75	0.004104138	14.8	9
>>END_MODULE