FastQCFastQC Report
Fri 10 Feb 2017
ERR1630814.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630814.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425549
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26110.1831575063361554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.17509043884145686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18310.12844174419820012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.020.181821
ATAACAC1156.408118E-817.6956523
CTTATAC8250.017.26666637
GTGTAAT752.067705E-417.2666661
TATATAG1107.8108314E-716.8181825
TCTAGCG1951.8189894E-1216.12820428
TACCGTC2200.015.9772737
CTAGCGG2003.6379788E-1215.72499929
CGCAATA2150.015.48837236
GTACAAC855.3652725E-415.2352946
CGAACGA1954.1836756E-1115.17948616
TAACACT1601.0968506E-815.031254
GTATTAG4100.014.89024451
TAGGGGT1252.9597668E-614.8000014
TTAACGG750.004105048714.835
TCGCACG2006.184564E-1114.79999913
TAACGAA2006.184564E-1114.79999913
ACGAACG2006.184564E-1114.79999915
ATACCGT2400.014.6458336
GATAACG2151.2732926E-1114.62790711
Produced by FastQC (version 0.11.5)