FastQCFastQC Report
Fri 10 Feb 2017
ERR1630813.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630813.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424446
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102350.2988804612483304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79350.23171631265319997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79240.2313950928120928No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.16280005583384877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG451.3239596E-424.6666665
GGTATCA51100.018.0655571
AAGACGG7450.016.3892615
GACGGAC6950.015.7050367
TACCGTC3450.015.0144937
GTATACG908.279912E-414.3888881
AGACGGA8250.014.3515166
TAATACG1551.2122837E-714.3225814
GTCTATA1702.4414476E-814.1470591
GCGAAAG7850.014.14012718
CAAGACG10400.013.8754
ATACCGT4050.013.7037046
ATACACT4900.013.5918384
ACCGTCG3850.013.4545458
CGAAAGC8150.013.39263719
ACGGACC8300.013.3734948
CGGACCA8600.013.3372119
TCTTATA29500.013.29491537
TTAACGG3350.013.25373235
TATTAGA6300.013.2142862
Produced by FastQC (version 0.11.5)