##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630813.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3424446
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06975697674894	33.0	31.0	34.0	30.0	34.0
2	32.24172581492014	33.0	31.0	34.0	30.0	34.0
3	32.354009670469324	34.0	31.0	34.0	30.0	34.0
4	35.92343958701641	37.0	35.0	37.0	35.0	37.0
5	35.894692163345546	37.0	35.0	37.0	35.0	37.0
6	35.92416963210984	37.0	35.0	37.0	35.0	37.0
7	35.89752882656056	37.0	35.0	37.0	35.0	37.0
8	35.8942182180709	37.0	35.0	37.0	35.0	37.0
9	37.62841055166295	39.0	37.0	39.0	35.0	39.0
10	37.538874609206864	39.0	37.0	39.0	35.0	39.0
11	37.63597294277673	39.0	37.0	39.0	35.0	39.0
12	37.5655104504495	39.0	37.0	39.0	35.0	39.0
13	37.61717982996374	39.0	37.0	39.0	35.0	39.0
14	38.91906077654605	40.0	38.0	41.0	36.0	41.0
15	38.886457838727786	40.0	38.0	41.0	35.0	41.0
16	38.87319846772296	40.0	38.0	41.0	35.0	41.0
17	38.856625860066124	40.0	38.0	41.0	35.0	41.0
18	38.84710899222823	40.0	38.0	41.0	35.0	41.0
19	38.874030135093385	40.0	38.0	41.0	35.0	41.0
20	38.84590208167978	40.0	38.0	41.0	35.0	41.0
21	38.782854511357456	40.0	38.0	41.0	35.0	41.0
22	38.73551897153583	40.0	38.0	41.0	35.0	41.0
23	38.71166372604503	40.0	38.0	41.0	35.0	41.0
24	38.669324030806735	40.0	38.0	41.0	34.0	41.0
25	38.62177882203428	40.0	38.0	41.0	34.0	41.0
26	38.448226662064464	40.0	38.0	41.0	34.0	41.0
27	38.34885087982114	40.0	38.0	41.0	34.0	41.0
28	38.260117110913704	40.0	38.0	41.0	34.0	41.0
29	38.16757455074485	40.0	38.0	41.0	33.0	41.0
30	38.09340459741517	40.0	38.0	41.0	33.0	41.0
31	38.05055912693615	40.0	37.0	41.0	33.0	41.0
32	37.95890342554679	40.0	37.0	41.0	33.0	41.0
33	37.926069793479	40.0	37.0	41.0	33.0	41.0
34	37.85868400319351	40.0	37.0	41.0	33.0	41.0
35	37.815715009084684	40.0	37.0	41.0	33.0	41.0
36	37.77199844880018	40.0	37.0	41.0	33.0	41.0
37	37.59560086507423	40.0	37.0	41.0	33.0	41.0
38	37.49073222354799	40.0	37.0	41.0	32.0	41.0
39	37.433669562901564	40.0	36.0	41.0	32.0	41.0
40	37.3349803150641	40.0	36.0	41.0	32.0	41.0
41	37.21252255109294	40.0	36.0	41.0	31.0	41.0
42	37.125118632327684	40.0	36.0	41.0	31.0	41.0
43	36.27881706997278	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	7.0
11	5.0
12	9.0
13	4.0
14	2.0
15	5.0
16	15.0
17	29.0
18	68.0
19	172.0
20	377.0
21	690.0
22	1402.0
23	2477.0
24	4107.0
25	6676.0
26	10353.0
27	15276.0
28	22636.0
29	32130.0
30	44216.0
31	58990.0
32	77545.0
33	102536.0
34	138997.0
35	191442.0
36	271412.0
37	421395.0
38	774346.0
39	1247119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.937209113532525	18.704923365706453	11.632304904209324	25.7255626165517
2	19.364854928359215	20.418222392760757	33.905805493793736	26.311117185086285
3	20.82999702725638	23.234006318102256	28.082031370913718	27.853965283727643
4	14.767060131770219	15.142332511594574	33.783566743350605	36.3070406132846
5	14.980612922498999	36.35592443274036	33.72422867815699	14.939233966603648
6	35.62620055915614	33.824244855956266	15.397527074452336	15.152027510435264
7	30.058088227993668	29.424934719367744	21.38386763873631	19.13310941390228
8	27.841817333373047	32.50187037552936	19.62261924994583	20.033693041151764
9	27.272002537052707	13.667407808445512	19.62282366257199	39.437765991929794
10	17.959401316300504	25.782739748268774	32.20258108902871	24.055277846402017
11	36.364509763039045	21.42524659463166	21.084373939609502	21.1258697027198
12	22.40996061844748	25.121435700840372	28.310944310408164	24.157659370303985
13	30.25797457457352	19.6800299960928	24.704171127242187	25.35782430209149
14	23.27237748821269	20.447394994694033	24.84863245032919	31.431595066764086
15	26.327674607805175	26.926194777198997	22.251161209725602	24.494969405270226
16	25.945715014925042	25.482457600441066	23.895543979960554	24.67628340467334
17	24.43107585869364	25.772694327783242	25.040021072021577	24.756208741501545
18	24.125537386193272	24.670676658355834	26.372236560307854	24.83154939514304
19	24.85774341309514	24.70776294910184	26.22935213462265	24.205141503180368
20	24.775540335575446	24.401465229704307	26.46235332664028	24.360641108079964
21	25.290601749888886	24.616478110619937	25.956432076896522	24.136488062594648
22	25.23170171175133	24.559563795136498	25.87495904447026	24.333775448641912
23	24.554132259641413	24.674764910879016	26.279316420816677	24.49178640866289
24	24.5656377703138	25.366584843212596	25.462804786526057	24.60497259994755
25	25.021156706807467	24.614901213218136	25.93917381088795	24.424768269086446
26	24.663054987580473	25.2679703519927	25.96843401823244	24.10054064219439
27	25.03272062108732	24.784096464070394	25.585831985670094	24.597350929172194
28	24.285329656242208	24.895822565168206	26.358073685495402	24.46077409309418
29	24.240183667664784	25.221977511106907	26.390283275017328	24.147555546210977
30	23.91718251652968	25.38539080481923	26.258145113107346	24.43928156554374
31	24.416066131572816	25.25322344110551	25.796639806847587	24.534070620474086
32	23.992318757545018	25.119128758345145	25.88520887758195	25.00334360652789
33	23.74991458472407	24.9875746325099	26.459841971518898	24.802668811247134
34	24.644249025973835	24.748674676137394	26.073414502667003	24.533661795221768
35	24.305332891802063	24.95930728649247	26.448774487902572	24.286585333802897
36	24.21734785714244	25.281724401552836	25.77000776183943	24.73091997946529
37	24.370423712331863	24.4648915474211	26.37848574630758	24.786198993939458
38	23.782036568834783	24.443165405440766	26.900175970069316	24.874622055655134
39	23.782416192283364	24.553694232585357	26.80500729169039	24.858882283440884
40	23.656848436214208	24.46021925882318	27.172891615169288	24.710040689793328
41	23.133931736695512	24.47958005470082	27.609750599075007	24.776737609528666
42	23.57940525270365	24.681919352794583	27.446979745044892	24.291695649456877
43	23.374320985058606	23.84975555170092	27.453170527437138	25.32275293580334
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	560.0
1	578.0
2	596.0
3	1323.5
4	2051.0
5	2051.0
6	2833.0
7	3615.0
8	3290.5
9	2966.0
10	4489.0
11	6012.0
12	6012.0
13	10320.5
14	14629.0
15	18597.5
16	22566.0
17	22568.0
18	22570.0
19	22570.0
20	26294.0
21	30018.0
22	28033.0
23	26048.0
24	29543.5
25	33039.0
26	33039.0
27	38948.0
28	44857.0
29	53453.5
30	62050.0
31	72803.5
32	83557.0
33	83557.0
34	99157.0
35	114757.0
36	128356.5
37	141956.0
38	160525.0
39	179094.0
40	179094.0
41	189771.0
42	200448.0
43	212041.0
44	223634.0
45	226571.5
46	229509.0
47	229509.0
48	238259.0
49	247009.0
50	246616.5
51	246224.0
52	244725.0
53	243226.0
54	243226.0
55	235504.5
56	227783.0
57	217287.5
58	206792.0
59	199540.5
60	192289.0
61	192289.0
62	180002.0
63	167715.0
64	150823.5
65	133932.0
66	118875.0
67	103818.0
68	103818.0
69	89436.0
70	75054.0
71	64309.5
72	53565.0
73	42530.0
74	31495.0
75	31495.0
76	25606.5
77	19718.0
78	16274.0
79	12830.0
80	10677.5
81	8525.0
82	8525.0
83	6318.5
84	4112.0
85	3487.0
86	2862.0
87	2350.0
88	1838.0
89	1838.0
90	1414.0
91	990.0
92	543.5
93	97.0
94	63.5
95	30.0
96	30.0
97	19.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3424446.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.431249514422646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51612219539135	40.60528648369524
2	10.773161062117472	10.866079471654173
3	3.3443971052492727	5.059863746704167
4	1.5742102853833602	3.1755756676135483
5	0.8885967868477307	2.2406523137616077
6	0.5470762535930169	1.6553843429019
7	0.39424366712905384	1.3917540522526413
8	0.2743739413851255	1.1069616558599071
9	0.2129482642046946	0.9665322341193137
>10	1.2194151050626143	11.660821675164726
>50	0.13608087641571248	4.823037601889514
>100	0.10687678247980155	10.521282607134342
>500	0.009358723876819531	3.137911162886649
>1k	0.002906435985269892	1.860478992921107
>5k	1.743861591161935E-4	0.6283385603761287
>10k+	5.8128719705397834E-5	0.3000394310651151
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10235	0.2988804612483304	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7935	0.23171631265319997	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7924	0.2313950928120928	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5575	0.16280005583384877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.9201803737013227E-5	0.0
2	0.0	5.8403607474026454E-5	0.0	6.716414859513042E-4	0.0
3	0.0	5.8403607474026454E-5	0.0	0.0013140811681655953	0.0
4	0.0	5.8403607474026454E-5	0.0	0.0021025298690649525	0.0
5	0.0	2.044126261590926E-4	0.0	0.0023653461026980713	0.0
6	0.0	2.044126261590926E-4	0.0	0.002423749710172098	0.0
7	0.0	2.044126261590926E-4	0.0	0.0028033731587532697	0.0
8	0.0	2.044126261590926E-4	0.0	0.0031537948035974287	0.0
9	0.0	2.044126261590926E-4	0.0	0.0049935084390292615	0.0
10	0.0	2.044126261590926E-4	0.0	0.007212845523042267	0.0
11	0.0	2.6281623363311906E-4	0.0	0.008118101438889677	0.0
12	0.0	2.6281623363311906E-4	0.0	0.008760541121103969	0.0
13	0.0	2.6281623363311906E-4	0.0	0.009694998840688392	0.0
14	0.0	2.6281623363311906E-4	0.0	0.010249833111691644	0.0
15	0.0	2.6281623363311906E-4	0.0	0.011417905261172171	0.0
16	0.0	2.6281623363311906E-4	0.0	0.013345224307815045	0.0
17	0.0	2.6281623363311906E-4	0.0	0.01582737762546117	0.0
18	0.0	2.6281623363311906E-4	0.0	0.016937046167467672	0.0
19	0.0	2.6281623363311906E-4	0.0	0.018601548980477427	0.0
20	0.0	2.6281623363311906E-4	0.0	0.020207648186013154	0.0
21	0.0	2.6281623363311906E-4	0.0	0.02341984659708461	0.0
22	0.0	2.6281623363311906E-4	0.0	0.027916924372584646	0.0
23	0.0	3.212198411071455E-4	0.0	0.03492535726946782	0.0
24	0.0	3.212198411071455E-4	0.0	0.04470796152136725	0.0
25	0.0	3.212198411071455E-4	0.0	0.04870860863333806	0.0
26	0.0	3.212198411071455E-4	0.0	0.05568783972648422	0.0
27	0.0	3.212198411071455E-4	0.0	0.07110639209962721	0.0
28	0.0	3.504216448441587E-4	0.0	0.10661578544383529	0.0
29	0.0	3.504216448441587E-4	0.0	0.15272543354457918	0.0
30	0.0	3.504216448441587E-4	0.0	0.21883831720517713	0.0
31	0.0	3.504216448441587E-4	0.0	0.42117761529894177	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	45	1.3239596E-4	24.666666	5
GGTATCA	5110	0.0	18.065557	1
AAGACGG	745	0.0	16.389261	5
GACGGAC	695	0.0	15.705036	7
TACCGTC	345	0.0	15.014493	7
GTATACG	90	8.279912E-4	14.388888	1
AGACGGA	825	0.0	14.351516	6
TAATACG	155	1.2122837E-7	14.322581	4
GTCTATA	170	2.4414476E-8	14.147059	1
GCGAAAG	785	0.0	14.140127	18
CAAGACG	1040	0.0	13.875	4
ATACCGT	405	0.0	13.703704	6
ATACACT	490	0.0	13.591838	4
ACCGTCG	385	0.0	13.454545	8
CGAAAGC	815	0.0	13.392637	19
ACGGACC	830	0.0	13.373494	8
CGGACCA	860	0.0	13.337211	9
TCTTATA	2950	0.0	13.294915	37
TTAACGG	335	0.0	13.253732	35
TATTAGA	630	0.0	13.214286	2
>>END_MODULE