FastQCFastQC Report
Fri 10 Feb 2017
ERR1630812.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630812.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011020
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50340.25032073276247874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46490.2311762190331275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36210.18005788107527523No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC34220.1701624051476365No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT30320.1507692613698521No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA26730.13291762389235315No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA26530.1319231036986206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23030.11451900030830126No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG21390.10636393471969449No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20360.10124215572197193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.019.5499041
GCCGTCT3150.019.38095336
GTACTAG701.219774E-418.51
AAGACGG5300.017.4528315
GACCCGT650.001580491317.0769236
CGGTCCA4100.016.69512210
CGCAAGA5750.016.4086952
CAAGACG6000.016.3416674
TCTAGCG4000.016.18749828
ACGGACC5400.016.1018528
ATGCCGT3950.015.9240534
AGAACCG3050.015.7704935
CTAGCGG4250.015.670588529
GCTTTAT8950.015.5027921
TACCGTC4200.015.4166687
ACCTAGG10950.015.3744291
GTCCTAA2650.015.3584911
AATTACG855.3666346E-415.23529416
ATACCGT4400.015.1363646
GCGCAAG6050.014.9834711
Produced by FastQC (version 0.11.5)