FastQCFastQC Report
Fri 10 Feb 2017
ERR1630811.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630811.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715729
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83340.30687892643190834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70450.2594146912302369No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65560.24140847632440499No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45310.16684286245056115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.017.1358361
TAACACG855.367496E-415.2352934
GTCTTAT1853.0559022E-1015.01
GCAGTCG4450.014.9662939
CGCCTTA3300.014.57575925
AGTCGGT4500.014.38888911
TCTAGCG2600.014.23076928
CTTATAC16650.013.88888937
TTAGGCG800.00630153613.87515
TAGATCG800.00630153613.87533
TAGACTG3100.013.7258065
CTCTATG9250.013.61
CAGTCGG4950.013.45454610
CTAGCGG2750.013.45454629
CGCAATA2651.8189894E-1213.26415236
GACGGAC4800.013.1041667
CTAGACT2152.904926E-912.9069774
TATACAG3300.012.893945
GCGCAAG5600.012.8839291
ACACCGT1153.5799638E-412.8695656
Produced by FastQC (version 0.11.5)