FastQCFastQC Report
Fri 10 Feb 2017
ERR1630810.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630810.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013578
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT227442.2439318927601035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219662.1671741099352984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168751.6648940683400786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104151.0275479538821877No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.2722040138992756No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26350.2599701256341397No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT25300.24961078476446805No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.24319785946419514No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19920.1965314953560555No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT16860.1663414162501554TruSeq Adapter, Index 8 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT15250.15045709358332562No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14400.14207096049835335No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.12875180795163274No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12650.12480539238223402No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA11930.11770184435731636No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11700.1154326554049121No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10310.1017188613012516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACTG1600.026.593755
CAGGACG358.868669E-426.4285724
GCCGTCT2450.023.40816336
ACGACTT400.001931030823.12525
CGCCGTA502.7017968E-422.225
ATACTGG2000.021.2750026
TGCATAA806.958453E-720.812514
CCGTCTT2850.020.7719337
ATGTACG450.003825501520.55555511
CTCTAAT1550.020.2903231
CAGTCGG3500.019.0285710
CTATATC701.21901685E-418.54
AGCGGTA609.235786E-418.514
CCGTAGT609.235786E-418.527
ATATACA701.21901685E-418.51
TCTAATA1800.018.52
GTACTAT902.1517062E-618.51
ATAACAC500.00703432218.53
CGATAGA701.21901685E-418.537
ATACCTT902.1517062E-618.56
Produced by FastQC (version 0.11.5)