##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630810.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1013578
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.849266657326815	33.0	31.0	34.0	30.0	34.0
2	31.94744361065453	33.0	31.0	34.0	30.0	34.0
3	31.985767252248962	33.0	31.0	34.0	30.0	34.0
4	35.64273297171012	37.0	35.0	37.0	33.0	37.0
5	35.66941863379039	37.0	35.0	37.0	33.0	37.0
6	35.75319314349759	37.0	35.0	37.0	35.0	37.0
7	35.71114211239786	37.0	35.0	37.0	33.0	37.0
8	35.7578854316096	37.0	35.0	37.0	35.0	37.0
9	37.3894924712257	39.0	37.0	39.0	34.0	39.0
10	37.3093930610175	39.0	37.0	39.0	34.0	39.0
11	37.46092061982403	39.0	37.0	39.0	35.0	39.0
12	37.39059944079291	39.0	37.0	39.0	34.0	39.0
13	37.46337923672377	39.0	37.0	39.0	35.0	39.0
14	38.553388096426715	40.0	38.0	41.0	34.0	41.0
15	38.518522501474976	40.0	38.0	41.0	34.0	41.0
16	38.441746959780104	40.0	38.0	41.0	34.0	41.0
17	38.552966816564684	40.0	38.0	41.0	34.0	41.0
18	38.62161767520605	40.0	38.0	41.0	34.0	41.0
19	38.69729315356095	40.0	38.0	41.0	34.0	41.0
20	38.69858264484825	40.0	38.0	41.0	34.0	41.0
21	38.631654396602926	40.0	38.0	41.0	34.0	41.0
22	38.530098324943914	40.0	38.0	41.0	34.0	41.0
23	38.47406711668959	40.0	38.0	41.0	34.0	41.0
24	38.392666375947385	40.0	38.0	41.0	34.0	41.0
25	38.307179121883074	40.0	37.0	41.0	34.0	41.0
26	38.02604831596582	40.0	37.0	41.0	33.0	41.0
27	37.8204844619753	40.0	36.0	41.0	33.0	41.0
28	37.68588209294203	40.0	36.0	41.0	33.0	41.0
29	37.51014327461725	39.0	36.0	41.0	33.0	41.0
30	37.29005957114302	39.0	35.0	41.0	32.0	41.0
31	37.07542488096624	39.0	35.0	41.0	32.0	41.0
32	36.81648378319182	39.0	35.0	41.0	31.0	41.0
33	36.64116131170961	39.0	35.0	41.0	31.0	41.0
34	36.44564503175878	39.0	35.0	41.0	31.0	41.0
35	36.24735146185099	39.0	35.0	41.0	30.0	41.0
36	36.053036865441044	38.0	35.0	41.0	30.0	41.0
37	35.697680888890645	38.0	35.0	40.0	28.0	41.0
38	35.43233969166655	38.0	35.0	40.0	26.0	41.0
39	35.16938311605027	38.0	35.0	40.0	25.0	41.0
40	34.85259940527517	38.0	35.0	40.0	23.0	41.0
41	34.513924927336625	38.0	34.0	40.0	21.0	41.0
42	34.1782260467374	38.0	33.0	40.0	18.0	41.0
43	33.2919962745837	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	10.0
11	22.0
12	5.0
13	9.0
14	4.0
15	6.0
16	12.0
17	25.0
18	52.0
19	111.0
20	224.0
21	508.0
22	893.0
23	1495.0
24	2466.0
25	4046.0
26	6048.0
27	8933.0
28	12943.0
29	17422.0
30	22322.0
31	27668.0
32	33870.0
33	42067.0
34	53522.0
35	67661.0
36	93354.0
37	156494.0
38	205042.0
39	256335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.08083640331578	20.149016651900496	13.199082853021672	19.571064091762054
2	19.034351574323832	23.33831239431006	33.698047905538594	23.929288125827515
3	22.911112908922647	26.499687246566122	29.438385600318874	21.150814244192357
4	13.69692317710132	16.48526309765997	32.38813391766593	37.429679807572775
5	12.41039170147734	41.43874472413568	31.303658919195165	14.847204655191806
6	31.669787623646133	32.96214006223497	16.910982677208857	18.457089636910034
7	26.178942321163245	34.0924921417	20.86469911541095	18.863866421725806
8	31.266365292064354	30.169853726106922	19.807454384369034	18.75632659745969
9	28.201973602426257	11.58716941370077	22.72543405638244	37.48542292749054
10	20.40010734250349	29.409478106273024	29.49383273906892	20.696581812154566
11	31.48124761981811	23.470418655495678	19.760590699482428	25.287743025203785
12	21.62990909431736	31.51577875605035	27.480174194783235	19.37413795484906
13	30.98113810678606	20.405139022354472	27.835844897975292	20.77787797288418
14	23.231857834325528	22.826856936515984	29.690068253257273	24.25121697590121
15	27.07102956062582	25.927062347446373	27.311958231137613	19.68994986079019
16	22.391961940768248	25.61884729147633	29.27549729769194	22.71369347006348
17	20.082519549556128	28.45543214237089	29.0959353892843	22.366112918788687
18	19.537026257475993	23.909852029148226	33.090990530575844	23.462131182799943
19	20.776398067045655	25.703695226218404	34.40149648078392	19.118410225952022
20	21.91770144971576	23.51511181181912	35.54279986345402	19.0243868750111
21	22.918019136169097	24.414894561642026	33.19221608993092	19.47487021225796
22	22.486774574823052	25.08252941559505	33.13341449794688	19.29728151163502
23	21.055015006245203	25.388869924169626	33.192906712655564	20.36320835692961
24	20.132639027287492	26.889001142487306	33.224576697599986	19.753783132625216
25	19.770259417627454	26.801094735678948	33.428705042927135	19.999940803766457
26	20.25093283398022	27.190408631600132	33.03051171197481	19.528146822444846
27	19.741055942413904	27.39848339249668	32.922182604594816	19.938278060494603
28	19.005641401056454	26.996935608310363	33.99580496024973	20.001618030383455
29	19.13942488885907	27.252564676818164	34.418466067732325	19.189544366590436
30	18.307717807608295	28.47812403189493	34.098609085832564	19.11554907466421
31	19.364370576314798	28.843463453232015	32.59640599934095	19.19575997111224
32	17.970299276424704	28.666072073387543	33.450508988948066	19.913119661239687
33	17.91475347728542	28.36160611220843	33.802035955792256	19.921604454713897
34	18.867220874959795	27.728995696433824	34.04178070163322	19.36200272697316
35	18.09471002725	28.70849604075858	33.183237994510534	20.013555937480884
36	18.54815317617391	29.51188759029892	31.81965275489405	20.12030647863312
37	18.308605751111408	28.6571926383564	32.54441197421412	20.489789636318072
38	18.305744599823594	27.95680253517736	32.63616613620264	21.101286728796403
39	18.287492427815128	28.39327609715286	32.94734100384973	20.371890471182287
40	17.431810871980254	28.29875944426576	33.370495413278505	20.898934270475483
41	17.56046401954265	28.147611727957788	32.878377391774485	21.413546860725074
42	17.27454621153971	28.745000384775516	33.238586472871354	20.741866930813416
43	17.133560515322944	27.792532987101144	32.62925990895619	22.444646588619722
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	493.0
1	481.0
2	469.0
3	1554.0
4	2639.0
5	2639.0
6	4023.5
7	5408.0
8	5551.5
9	5695.0
10	8143.5
11	10592.0
12	10592.0
13	18833.0
14	27074.0
15	37566.0
16	48058.0
17	42868.0
18	37678.0
19	37678.0
20	40490.0
21	43302.0
22	31165.5
23	19029.0
24	17538.0
25	16047.0
26	16047.0
27	16598.5
28	17150.0
29	18314.5
30	19479.0
31	21845.5
32	24212.0
33	24212.0
34	27640.0
35	31068.0
36	33615.5
37	36163.0
38	41227.0
39	46291.0
40	46291.0
41	49161.0
42	52031.0
43	55176.0
44	58321.0
45	59132.5
46	59944.0
47	59944.0
48	62837.0
49	65730.0
50	65664.5
51	65599.0
52	61833.0
53	58067.0
54	58067.0
55	58828.5
56	59590.0
57	55995.0
58	52400.0
59	48493.0
60	44586.0
61	44586.0
62	39880.0
63	35174.0
64	30158.5
65	25143.0
66	21683.0
67	18223.0
68	18223.0
69	14505.0
70	10787.0
71	9121.0
72	7455.0
73	5744.5
74	4034.0
75	4034.0
76	3146.5
77	2259.0
78	1697.0
79	1135.0
80	975.5
81	816.0
82	816.0
83	684.0
84	552.0
85	491.5
86	431.0
87	357.0
88	283.0
89	283.0
90	201.0
91	119.0
92	80.0
93	41.0
94	24.0
95	7.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1013578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.38496568424867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.65150237142792	37.51135598878854
2	9.911932150132845	8.997054009967606
3	2.7334933571077737	3.7217850663137404
4	1.1492497091825826	2.086346344555371
5	0.6353275084914267	1.4417158585571304
6	0.4314750203866282	1.174948739631457
7	0.31116146857339577	0.9885436801424792
8	0.24759292635954172	0.8989597173192407
9	0.186319363413362	0.7610488123343843
>10	1.4245570567858403	12.979964658360323
>50	0.1760138361861326	5.591947134366497
>100	0.12656241875051233	11.069568371630108
>500	0.011109609901533136	3.3850237245315697
>1k	0.0028318613474496226	2.2734894584925955
>5k	0.0	0.0
>10k+	8.713419530614224E-4	7.118248435008996
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22744	2.2439318927601035	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21966	2.1671741099352984	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16875	1.6648940683400786	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10415	1.0275479538821877	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2759	0.2722040138992756	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2635	0.2599701256341397	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	2530	0.24961078476446805	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2465	0.24319785946419514	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1992	0.1965314953560555	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	1686	0.1663414162501554	TruSeq Adapter, Index 8 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1525	0.15045709358332562	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1440	0.14207096049835335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1305	0.12875180795163274	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1265	0.12480539238223402	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1193	0.11770184435731636	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1170	0.1154326554049121	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1031	0.1017188613012516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.866038923496762E-5
2	0.0	0.0	0.0	7.892831138797409E-4	9.866038923496762E-5
3	0.0	0.0	0.0	0.0015785662277594818	9.866038923496762E-5
4	0.0	0.0	0.0	0.0023678493416392225	9.866038923496762E-5
5	0.0	0.0	0.0	0.0028611512878140605	9.866038923496762E-5
6	0.0	0.0	0.0	0.0028611512878140605	9.866038923496762E-5
7	0.0	0.0	0.0	0.003354453233988899	9.866038923496762E-5
8	0.0	0.0	0.0	0.003551774012458834	9.866038923496762E-5
9	0.0	0.0	0.0	0.0051303402402183155	9.866038923496762E-5
10	0.0	9.866038923496762E-5	0.0	0.007103548024917668	9.866038923496762E-5
11	0.0	9.866038923496762E-5	0.0	0.00907675580961702	9.866038923496762E-5
12	0.0	1.9732077846993523E-4	0.0	0.011839246708196112	9.866038923496762E-5
13	0.0	1.9732077846993523E-4	0.0	0.01282585060054579	9.866038923496762E-5
14	0.0	1.9732077846993523E-4	0.0	0.014799058385245142	9.866038923496762E-5
15	0.0	1.9732077846993523E-4	0.0	0.019238775900818683	9.866038923496762E-5
16	0.0	1.9732077846993523E-4	0.0	0.03097936221977983	9.866038923496762E-5
17	0.0	1.9732077846993523E-4	0.0	0.04558109982655503	9.866038923496762E-5
18	0.0	1.9732077846993523E-4	0.0	0.051106081623713225	9.866038923496762E-5
19	9.866038923496762E-5	1.9732077846993523E-4	0.0	0.05850561081633579	9.866038923496762E-5
20	9.866038923496762E-5	1.9732077846993523E-4	0.0	0.06531317767354856	9.866038923496762E-5
21	9.866038923496762E-5	1.9732077846993523E-4	0.0	0.08603185941289175	9.866038923496762E-5
22	9.866038923496762E-5	1.9732077846993523E-4	0.0	0.12598931705305363	1.9732077846993523E-4
23	9.866038923496762E-5	1.9732077846993523E-4	0.0	0.17768736101217666	1.9732077846993523E-4
24	9.866038923496762E-5	2.959811677049028E-4	0.0	0.26302859770042364	1.9732077846993523E-4
25	9.866038923496762E-5	2.959811677049028E-4	0.0	0.2819713924335374	1.9732077846993523E-4
26	1.9732077846993523E-4	2.959811677049028E-4	0.0	0.3104842449224431	1.9732077846993523E-4
27	1.9732077846993523E-4	2.959811677049028E-4	0.0	0.3420555694776327	1.9732077846993523E-4
28	1.9732077846993523E-4	2.959811677049028E-4	0.0	0.39424691538293055	1.9732077846993523E-4
29	1.9732077846993523E-4	2.959811677049028E-4	0.0	0.48254796374822656	1.9732077846993523E-4
30	1.9732077846993523E-4	2.959811677049028E-4	0.0	0.6402072657457049	1.9732077846993523E-4
31	1.9732077846993523E-4	2.959811677049028E-4	0.0	1.078160733559726	1.9732077846993523E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACTG	160	0.0	26.59375	5
CAGGACG	35	8.868669E-4	26.428572	4
GCCGTCT	245	0.0	23.408163	36
ACGACTT	40	0.0019310308	23.125	25
CGCCGTA	50	2.7017968E-4	22.2	25
ATACTGG	200	0.0	21.275002	6
TGCATAA	80	6.958453E-7	20.8125	14
CCGTCTT	285	0.0	20.77193	37
ATGTACG	45	0.0038255015	20.555555	11
CTCTAAT	155	0.0	20.290323	1
CAGTCGG	350	0.0	19.02857	10
CTATATC	70	1.21901685E-4	18.5	4
AGCGGTA	60	9.235786E-4	18.5	14
CCGTAGT	60	9.235786E-4	18.5	27
ATATACA	70	1.21901685E-4	18.5	1
TCTAATA	180	0.0	18.5	2
GTACTAT	90	2.1517062E-6	18.5	1
ATAACAC	50	0.007034322	18.5	3
CGATAGA	70	1.21901685E-4	18.5	37
ATACCTT	90	2.1517062E-6	18.5	6
>>END_MODULE