FastQCFastQC Report
Fri 10 Feb 2017
ERR1630809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074530
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74920.36114204181188025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69880.33684738229864114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62270.3001643745812304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42330.20404621769750256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG1101.7571438E-920.18181835
CGAACGA1501.2732926E-1118.516
ACGAACG1705.456968E-1217.41176415
GGTATCA34800.016.2672421
GCGTTAT2950.015.0508471
ACAGTCG750.00410588614.88
TAACGAA2059.276846E-1114.43902413
TAAACCG908.277973E-414.3888885
GTATAGA1803.3342076E-914.3888881
ATGCCGT2353.6379788E-1214.17021434
CTTATAC15200.013.87537
TACTCCG3200.013.8755
CAAGACG4200.013.6547614
CGTTATT2850.013.6315792
CGCAATA2850.013.63157936
GCCGTCT2601.8189894E-1213.51923136
TCTAGCG2750.013.45454628
CTAGCGG2750.013.45454629
TAATACT4400.013.4545454
GTCTACG1102.457854E-413.4545451
Produced by FastQC (version 0.11.5)