FastQCFastQC Report
Fri 10 Feb 2017
ERR1630808.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630808.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767152
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92601.2070619642521951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73260.9549606857571903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60410.7874580265710055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41770.544481406553069No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT18660.2432373245458527No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG16940.220816735145056No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT14340.1869251465159447Illumina PCR Primer Index 10 (95% over 21bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13450.17532379502367199No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG12060.15720483033349325No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12000.1564227167497445No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.14912298996808976No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.1487319331762154No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC11330.14768911506455046No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10400.13556635451644525No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.13478424093269653No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10220.13322001376519907No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9900.12904874131853922No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9730.12683275283125117No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT9670.12605063924750246No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG9340.12174901453688447No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9160.11940267378563832No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA8910.1161438671866853No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT8830.11510104907502033No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG8810.1148403445471041No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG8750.11405823096335536No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT8550.11145118568419296No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8440.11001731078065363No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8370.1091048449329468No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8350.10884414040503054No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG8320.1084530836131562No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8280.10793167455732372No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC8240.10741026550149123No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT8170.10649779965378439No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT8070.1051942770142032No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC8050.10493357248628693No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8020.10454251569441259No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7960.10376040211066385No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7870.10258723173504078No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7780.10141406135941769No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC7740.10089265230358521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGC250.00549521929.5999981
CAAAACG250.00549521929.59999814
GTCAAGA250.00549521929.5999981
AGACTAT405.9375925E-527.7500026
CAAGACT405.9375925E-527.7500024
CGTCTTC509.085945E-625.89999837
CCGTCTT1700.025.02941137
AATACTG2050.023.4634135
TTAGAGT400.001930604623.1250024
AACGCAC400.001930604623.12500232
ATGAAAG400.001930604623.12500219
ACGAACG400.001930604623.12500229
CTCTAAT2100.022.9047641
ATTAGAC652.6809157E-622.769233
TTAGACT652.6809157E-622.769234
GGTATTG753.7374048E-722.233
TGATGCT2200.021.86363613
GCCGTCT1950.021.82051336
TGCCCCA450.003824665420.55555512
CCGGACC450.003824665420.55555534
Produced by FastQC (version 0.11.5)