##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630808.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767152
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.860832012430393	33.0	31.0	34.0	30.0	34.0
2	32.01156876342628	33.0	31.0	34.0	30.0	34.0
3	32.078644127891216	33.0	31.0	34.0	30.0	34.0
4	35.69488706279851	37.0	35.0	37.0	33.0	37.0
5	35.701924781529605	37.0	35.0	37.0	35.0	37.0
6	35.77111445971594	37.0	35.0	37.0	35.0	37.0
7	35.74705143178927	37.0	35.0	37.0	35.0	37.0
8	35.75992502137777	37.0	35.0	37.0	35.0	37.0
9	37.42287577950654	39.0	37.0	39.0	34.0	39.0
10	37.33344760881807	39.0	37.0	39.0	34.0	39.0
11	37.46108854568586	39.0	37.0	39.0	35.0	39.0
12	37.40766888439318	39.0	37.0	39.0	34.0	39.0
13	37.45757555217219	39.0	37.0	39.0	35.0	39.0
14	38.64608838926314	40.0	38.0	41.0	34.0	41.0
15	38.59873532233508	40.0	38.0	41.0	34.0	41.0
16	38.5348561953824	40.0	38.0	41.0	34.0	41.0
17	38.57654024235093	40.0	38.0	41.0	34.0	41.0
18	38.59605267274282	40.0	38.0	41.0	34.0	41.0
19	38.66615090620894	40.0	38.0	41.0	34.0	41.0
20	38.63899852962646	40.0	38.0	41.0	34.0	41.0
21	38.56462474190252	40.0	38.0	41.0	34.0	41.0
22	38.50029068554863	40.0	38.0	41.0	34.0	41.0
23	38.45064472229754	40.0	38.0	41.0	34.0	41.0
24	38.402398742361356	40.0	38.0	41.0	34.0	41.0
25	38.327828122718834	40.0	38.0	41.0	34.0	41.0
26	38.07653242121509	40.0	37.0	41.0	33.0	41.0
27	37.9143611696248	40.0	37.0	41.0	33.0	41.0
28	37.78588337122239	40.0	37.0	41.0	33.0	41.0
29	37.67506830458631	40.0	37.0	41.0	33.0	41.0
30	37.52412429349073	40.0	36.0	41.0	32.0	41.0
31	37.42875727365633	39.0	36.0	41.0	32.0	41.0
32	37.25760605460196	39.0	36.0	41.0	32.0	41.0
33	37.1609394226959	39.0	35.0	41.0	32.0	41.0
34	37.03584556906584	39.0	35.0	41.0	31.0	41.0
35	36.89594369825015	39.0	35.0	41.0	31.0	41.0
36	36.80382766387886	39.0	35.0	41.0	31.0	41.0
37	36.52571850167894	39.0	35.0	41.0	30.0	41.0
38	36.343291811792184	39.0	35.0	41.0	30.0	41.0
39	36.18049617285753	39.0	35.0	41.0	30.0	41.0
40	35.97340944167518	39.0	35.0	41.0	29.0	41.0
41	35.76202238930486	39.0	35.0	41.0	27.0	41.0
42	35.53069404759422	39.0	35.0	41.0	26.0	41.0
43	34.681233966671535	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	2.0
11	9.0
12	3.0
13	2.0
14	1.0
15	3.0
16	5.0
17	6.0
18	35.0
19	73.0
20	147.0
21	298.0
22	541.0
23	956.0
24	1587.0
25	2621.0
26	3824.0
27	5678.0
28	8013.0
29	10951.0
30	14298.0
31	17901.0
32	22923.0
33	28954.0
34	37490.0
35	48368.0
36	66383.0
37	105940.0
38	167772.0
39	222363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10299393079859	20.681950904123305	12.639216217907274	22.575838947170833
2	19.78799507789851	21.384288902329658	33.927044444907914	24.90067157486391
3	21.394195674390474	25.522842930736022	29.040268421381942	24.042692973491565
4	13.577231109349908	16.71859031847665	34.80118672701107	34.90299184516237
5	12.702567418190919	39.707776294658686	33.84752956389347	13.742126723256929
6	31.761371931507703	35.15053079441884	16.363771455982647	16.72432581809081
7	27.688906500928105	32.01738377792145	21.981302271257846	18.31240744989259
8	28.683363922664608	31.621112895488768	19.61905854380879	20.076464638037834
9	27.248185496485704	12.978783865518176	21.898267879116524	37.8747627588796
10	20.197040482199093	27.242319644607587	31.058251819717604	21.502388053475713
11	32.864412789121324	23.603666548480614	20.660051723778338	22.871868938619727
12	22.11295805785555	29.243096544100776	26.410932905082696	22.233012492960977
13	29.13816297161449	20.465566145952824	27.59635639351784	22.799914488914844
14	22.70358416584979	22.31748076000584	26.774224664733975	28.20471040941039
15	26.75336830250068	26.67633011450143	25.323664671408014	21.24663691158988
16	24.59838467474503	25.452322355934676	27.135561140425885	22.813731828894404
17	22.7334348342962	27.723449850877007	26.463725571985737	23.07938974284105
18	21.425871274532295	24.54298496256283	29.83737251548585	24.193771247419026
19	22.208245562808933	25.59505808496882	30.452765553632133	21.74393079859011
20	22.457348739232902	24.05351221140009	31.612770350595447	21.87636869877156
21	22.74686111748389	25.774944209231027	30.196101945898597	21.28209272738649
22	24.28775522973283	25.31753811500198	28.66733059419776	21.72737606106743
23	22.522264166684046	25.20256741819092	30.2057480134315	22.069420401693538
24	21.3962813106138	26.914614053016873	28.40115127119528	23.287953365174047
25	22.039048324191292	25.782243936012684	30.341575072475855	21.837132667320166
26	21.770131603645694	27.288203641520848	29.429239577032973	21.512425177800488
27	22.396734936492376	26.68297807996329	29.004030492001586	21.916256491542747
28	21.282353431914405	26.259724278891277	30.877583581871647	21.580338707322667
29	21.726985004275555	26.57674098483743	30.512988299580783	21.183285711306237
30	19.724643877614866	27.244665985358836	31.219627922497757	21.811062214528544
31	21.84091288297495	27.5659843160156	29.10974617807162	21.483356622937826
32	20.52474607378981	26.851132500469266	29.75498988466432	22.869131541076605
33	20.168362984128308	26.911876655473755	30.20965858135024	22.710101779047697
34	21.789163034183577	26.201717521429913	30.273791895217638	21.735327549168872
35	20.334562120674914	27.624512482532797	30.531759025590755	21.509166371201534
36	21.21756835672722	27.886781237616535	28.52498592195549	22.370664483700754
37	21.107420693682606	25.91807099505704	30.355392412455416	22.61911589880493
38	20.68364548355476	25.91051056374747	30.073310113250045	23.332533839447724
39	20.664614053016873	26.92921350658018	30.217740421715643	22.1884320186873
40	20.06694892276889	26.63305316286733	30.4024495797443	22.897548334619476
41	20.359459403090913	26.210190418587192	30.03615971802198	23.394190460299917
42	19.637438213026883	27.281946732850855	30.626785826016228	22.453829228106034
43	19.99838363192692	25.27061129997706	30.310029824597994	24.42097524349803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	212.0
1	191.0
2	170.0
3	503.5
4	837.0
5	837.0
6	1303.0
7	1769.0
8	1868.0
9	1967.0
10	2930.0
11	3893.0
12	3893.0
13	7181.5
14	10470.0
15	14009.5
16	17549.0
17	16118.5
18	14688.0
19	14688.0
20	16158.0
21	17628.0
22	12818.0
23	8008.0
24	7246.0
25	6484.0
26	6484.0
27	7366.0
28	8248.0
29	10224.0
30	12200.0
31	14773.5
32	17347.0
33	17347.0
34	22835.5
35	28324.0
36	31052.5
37	33781.0
38	40676.0
39	47571.0
40	47571.0
41	50895.0
42	54219.0
43	58249.0
44	62279.0
45	60817.5
46	59356.0
47	59356.0
48	61377.0
49	63398.0
50	62528.0
51	61658.0
52	56207.5
53	50757.0
54	50757.0
55	47248.5
56	43740.0
57	37404.0
58	31068.0
59	28979.0
60	26890.0
61	26890.0
62	25046.5
63	23203.0
64	21146.5
65	19090.0
66	17678.5
67	16267.0
68	16267.0
69	12821.0
70	9375.0
71	8029.5
72	6684.0
73	4942.0
74	3200.0
75	3200.0
76	2652.5
77	2105.0
78	1576.0
79	1047.0
80	812.5
81	578.0
82	578.0
83	508.0
84	438.0
85	387.0
86	336.0
87	261.0
88	186.0
89	186.0
90	144.0
91	102.0
92	63.5
93	25.0
94	15.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	767152.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.630354220739342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.99354071789061	19.477413670877148
2	9.858689845821054	5.053634258015996
3	3.5273362501684185	2.712206326424131
4	1.9502303397120155	1.999403776754071
5	1.2010187902317393	1.539126850970166
6	0.8874660576814236	1.364764165035479
7	0.7199957691168348	1.29176226199287
8	0.5422498495567886	1.1118444576226487
9	0.4530116627712081	1.0449764444656962
>10	3.7730687225283526	19.980492021424574
>50	0.6137532778237104	10.981455257543109
>100	0.4304975034609528	20.72373959827948
>500	0.0409510110307684	7.200573576178154
>1k	0.006654539292499865	2.549976277291668
>5k	0.0015356629136538146	2.968631057124849
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9260	1.2070619642521951	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7326	0.9549606857571903	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6041	0.7874580265710055	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4177	0.544481406553069	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	1866	0.2432373245458527	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1694	0.220816735145056	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	1434	0.1869251465159447	Illumina PCR Primer Index 10 (95% over 21bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1345	0.17532379502367199	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	1206	0.15720483033349325	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1200	0.1564227167497445	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1144	0.14912298996808976	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1141	0.1487319331762154	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1133	0.14768911506455046	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1040	0.13556635451644525	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1034	0.13478424093269653	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1022	0.13322001376519907	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	990	0.12904874131853922	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	973	0.12683275283125117	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT	967	0.12605063924750246	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	934	0.12174901453688447	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	916	0.11940267378563832	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	891	0.1161438671866853	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	883	0.11510104907502033	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	881	0.1148403445471041	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	875	0.11405823096335536	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	855	0.11145118568419296	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	844	0.11001731078065363	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	837	0.1091048449329468	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	835	0.10884414040503054	No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG	832	0.1084530836131562	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	828	0.10793167455732372	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	824	0.10741026550149123	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	817	0.10649779965378439	No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT	807	0.1051942770142032	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	805	0.10493357248628693	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	802	0.10454251569441259	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	796	0.10376040211066385	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	787	0.10258723173504078	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	778	0.10141406135941769	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	774	0.10089265230358521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3035226395812042E-4	0.0
2	0.0	0.0	0.0	2.6070452791624084E-4	0.0
3	0.0	0.0	0.0	5.214090558324817E-4	0.0
4	0.0	0.0	0.0	0.0010428181116649633	0.0
5	0.0	0.0	0.0	0.001173170375623084	0.0
6	0.0	0.0	0.0	0.001173170375623084	0.0
7	0.0	0.0	0.0	0.001564227167497445	0.0
8	0.0	0.0	0.0	0.0016945794314555656	0.0
9	0.0	0.0	0.0	0.003258806598953011	0.0
10	0.0	1.3035226395812042E-4	0.0	0.004692681502492336	0.0
11	0.0	1.3035226395812042E-4	0.0	0.0053444428222829375	0.0
12	0.0	1.3035226395812042E-4	0.0	0.00625690866998978	0.0
13	0.0	1.3035226395812042E-4	0.0	0.006517613197906022	0.0
14	0.0	1.3035226395812042E-4	0.0	0.007430079045612864	0.0
15	0.0	1.3035226395812042E-4	0.0	0.009124658477068429	0.0
16	0.0	1.3035226395812042E-4	0.0	0.013035226395812043	0.0
17	0.0	2.6070452791624084E-4	0.0	0.021117066761215508	0.0
18	0.0	2.6070452791624084E-4	0.0	0.024636577888084762	0.0
19	0.0	2.6070452791624084E-4	0.0	0.0299810207103677	0.0
20	0.0	2.6070452791624084E-4	0.0	0.03154524787786514	0.0
21	0.0	2.6070452791624084E-4	0.0	0.03897532692347801	0.0
22	0.0	2.6070452791624084E-4	0.0	0.04940350804012764	0.0
23	0.0	2.6070452791624084E-4	0.0	0.06621895009072518	0.0
24	0.0	2.6070452791624084E-4	0.0	0.09802490249650656	0.0
25	0.0	2.6070452791624084E-4	0.0	0.10688885644565875	0.0
26	0.0	2.6070452791624084E-4	0.0	0.12331324170438192	0.0
27	0.0	2.6070452791624084E-4	0.0	0.14390889940976495	0.0
28	0.0	2.6070452791624084E-4	0.0	0.18392704444490793	0.0
29	0.0	2.6070452791624084E-4	0.0	0.2399785179468997	0.0
30	0.0	2.6070452791624084E-4	0.0	0.33774271591549004	0.0
31	0.0	2.6070452791624084E-4	0.0	0.6524130811103928	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTCGC	25	0.005495219	29.599998	1
CAAAACG	25	0.005495219	29.599998	14
GTCAAGA	25	0.005495219	29.599998	1
AGACTAT	40	5.9375925E-5	27.750002	6
CAAGACT	40	5.9375925E-5	27.750002	4
CGTCTTC	50	9.085945E-6	25.899998	37
CCGTCTT	170	0.0	25.029411	37
AATACTG	205	0.0	23.463413	5
TTAGAGT	40	0.0019306046	23.125002	4
AACGCAC	40	0.0019306046	23.125002	32
ATGAAAG	40	0.0019306046	23.125002	19
ACGAACG	40	0.0019306046	23.125002	29
CTCTAAT	210	0.0	22.904764	1
ATTAGAC	65	2.6809157E-6	22.76923	3
TTAGACT	65	2.6809157E-6	22.76923	4
GGTATTG	75	3.7374048E-7	22.2	33
TGATGCT	220	0.0	21.863636	13
GCCGTCT	195	0.0	21.820513	36
TGCCCCA	45	0.0038246654	20.555555	12
CCGGACC	45	0.0038246654	20.555555	34
>>END_MODULE