FastQCFastQC Report
Fri 10 Feb 2017
ERR1630807.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630807.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960787
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106601.1095071019903475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77690.8086079432798321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68630.7143102477448174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49570.5159312105596766No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14910.15518528040033847No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.14488122757697594No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.13540982548681446No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT11810.12292006448879927TruSeq Adapter, Index 3 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11570.12042211228919626No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT11220.11677926533144184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG1400.023.7857155
GCCGTCT2050.023.46341536
ACGGACC1400.022.4642878
CGGACCA1400.022.4642879
CGCAAGA1750.019.028572
AGCGTCA701.2189329E-418.53
TAGGCGA500.007034061518.533
GCAGCGT701.2189329E-418.51
GGTATCA42000.018.323811
CCGTCTT2850.017.52631637
GCGCTTT953.6051424E-617.52631613
TATGCCG2750.017.4909133
TGCCGTC2750.017.4909135
GACGGAC1800.017.4722217
GCGCAAG2050.017.1463431
ATGCCGT2900.016.58620634
GCGAAAG2003.6379788E-1215.72518
GCGTTAT855.3630135E-415.2352941
GTTCCGG855.3630135E-415.23529426
CGAAAGC2109.094947E-1214.9761919
Produced by FastQC (version 0.11.5)