##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630807.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 960787 Sequences flagged as poor quality 0 Sequence length 43 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43362472639617 31.0 31.0 34.0 30.0 34.0 2 31.576406633312065 31.0 31.0 34.0 30.0 34.0 3 31.6354467743631 31.0 31.0 34.0 28.0 34.0 4 35.35422939735862 37.0 35.0 37.0 33.0 37.0 5 35.29066692201289 37.0 35.0 37.0 33.0 37.0 6 35.34834671992856 37.0 35.0 37.0 33.0 37.0 7 35.32154473364024 37.0 35.0 37.0 32.0 37.0 8 35.332815702127526 37.0 35.0 37.0 33.0 37.0 9 36.91573782742689 39.0 37.0 39.0 33.0 39.0 10 36.77258018686764 38.0 37.0 39.0 32.0 39.0 11 36.94310081214671 39.0 37.0 39.0 33.0 39.0 12 36.85705884863138 39.0 37.0 39.0 33.0 39.0 13 36.94200483561913 39.0 37.0 39.0 33.0 39.0 14 38.02473493084315 40.0 37.0 41.0 33.0 41.0 15 37.9674152543696 40.0 37.0 41.0 33.0 41.0 16 37.907237504254326 40.0 37.0 41.0 33.0 41.0 17 37.92697340825802 40.0 37.0 41.0 33.0 41.0 18 37.9382683154539 40.0 37.0 41.0 33.0 41.0 19 37.99609070480762 40.0 37.0 41.0 33.0 41.0 20 37.997394844018494 40.0 37.0 41.0 33.0 41.0 21 37.92789348731821 40.0 37.0 41.0 33.0 41.0 22 37.83129247169248 40.0 37.0 41.0 33.0 41.0 23 37.786564555931754 40.0 37.0 41.0 33.0 41.0 24 37.720949596528676 39.0 37.0 41.0 33.0 41.0 25 37.655299249469444 39.0 37.0 41.0 32.0 41.0 26 37.38260717515953 39.0 36.0 40.0 32.0 41.0 27 37.21782663587247 39.0 36.0 40.0 32.0 41.0 28 37.101274267865826 39.0 36.0 40.0 31.0 41.0 29 36.95385657799283 39.0 36.0 40.0 31.0 41.0 30 36.832291652572316 39.0 35.0 40.0 31.0 41.0 31 36.70579223074417 39.0 35.0 40.0 31.0 41.0 32 36.55138651959279 38.0 35.0 40.0 30.0 41.0 33 36.456081316670605 38.0 35.0 40.0 30.0 41.0 34 36.332183928383714 38.0 35.0 40.0 30.0 41.0 35 36.23471071111495 38.0 35.0 40.0 30.0 41.0 36 36.13749249313323 38.0 35.0 40.0 30.0 41.0 37 35.854429753941304 38.0 35.0 40.0 29.0 41.0 38 35.66574485291745 38.0 35.0 40.0 27.0 41.0 39 35.514387684263006 38.0 35.0 40.0 27.0 41.0 40 35.32324230032255 38.0 34.0 40.0 26.0 41.0 41 35.09985667999255 38.0 34.0 40.0 25.0 41.0 42 34.92373543771929 38.0 34.0 40.0 24.0 41.0 43 34.07533407508636 37.0 33.0 40.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 1.0 13 5.0 14 5.0 15 12.0 16 16.0 17 31.0 18 73.0 19 170.0 20 285.0 21 582.0 22 1060.0 23 1765.0 24 2863.0 25 4316.0 26 6518.0 27 9202.0 28 13068.0 29 17244.0 30 22309.0 31 27893.0 32 35105.0 33 44156.0 34 56863.0 35 73479.0 36 103450.0 37 157523.0 38 237243.0 39 145548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.999332838600026 21.82887570293936 13.258922112809604 21.91286934565101 2 18.837057537206476 23.246671738897383 33.9215663825593 23.994704341336842 3 22.035269003431562 25.141264400954633 29.85781447917176 22.965652116442044 4 14.79599536629867 17.105976662881574 34.27637967624458 33.821648294575176 5 13.233318102763672 39.13219058958958 33.53344705954598 14.101044248100775 6 32.73941050409716 34.5806094378879 16.28591977202023 16.394060285994712 7 26.54178293419874 33.035001514383524 22.011642538876984 18.41157301254076 8 27.60632689659623 32.62835571255648 20.962190371018757 18.803127019828537 9 26.361305887777416 13.777351275568883 22.70107734596742 37.16026549068628 10 18.003990478638865 28.422636859158168 32.465468412874024 21.107904249328936 11 32.3196504532222 23.098355827045953 21.64069663723593 22.94129708249591 12 20.5413895067273 28.708340141987765 29.40849532726817 21.34177502401677 13 29.284014042654615 21.165253068578156 27.131507815988353 22.41922507277888 14 22.13352178994928 23.596697290866757 27.894215887600478 26.37556503158348 15 25.84017061013523 27.706661309946952 25.38273311358293 21.07043496633489 16 23.093255841305098 27.311256293018122 27.511925119719567 22.08356274595722 17 20.797221444503307 28.032435909311847 28.402965485586297 22.76737716059855 18 20.636936178362113 25.4001146976385 30.80204041062171 23.16090871337768 19 21.2440426442073 26.832065796060938 31.259998313882264 20.663893245849497 20 22.37165989964477 25.447159464064356 31.458169188384105 20.723011447906767 21 23.31848786463597 26.098604581452495 29.946699944940967 20.636207608970565 22 22.605218430307655 26.19987572687807 30.59117161243855 20.60373423037572 23 21.90995506808481 26.401064960287762 30.362504904833227 21.326475066794202 24 21.535678563510956 27.089354872620046 30.38290484779665 20.992061716072342 25 21.65443537433375 27.05646516865861 30.343978426019504 20.945121030988137 26 21.20875906938791 27.463839539877206 30.213564504931895 21.113836885802993 27 21.25809362533007 27.043559082293996 30.53913094161349 21.159216350762446 28 20.807629578668323 27.100699738859912 30.900813603847677 21.190857078624088 29 20.48674680236098 27.39514585438812 31.163410828830944 20.95469651441995 30 20.524112004013375 28.132874404004216 30.921109465469453 20.421904126512953 31 21.121226661060152 28.369451293574954 29.804108506880294 20.705213538484596 32 20.36080837896433 27.90254239493249 30.401639489293675 21.335009736809514 33 19.98309719011602 27.847795609224523 30.865113703661684 21.303993496997773 34 20.739768543912437 27.383905069489906 30.715756978393756 21.160569408203898 35 20.369447130321287 27.61683911210289 30.857619847062878 21.156093910512944 36 20.259016826830504 28.535981440215156 29.769033094744206 21.435968638210134 37 20.85561107716903 27.647334945206374 30.135399417352648 21.361654560271944 38 20.43023063384496 27.4605089369444 30.4660658397751 21.643194589435534 39 20.550132339425907 27.476849707583472 30.5894022296305 21.38361572336012 40 19.917317782193138 27.101428308251464 31.22554738979607 21.755706519759322 41 19.775142669499065 27.323537891332833 30.801520003913456 22.099799435254642 42 19.828952723132183 27.316356278758974 31.079417186119297 21.775273811989546 43 19.574369761455973 26.716327344145995 30.771024170809973 22.93827872358806 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 221.0 1 231.5 2 242.0 3 718.5 4 1195.0 5 1195.0 6 1835.5 7 2476.0 8 2589.0 9 2702.0 10 3950.5 11 5199.0 12 5199.0 13 9377.5 14 13556.0 15 17712.5 16 21869.0 17 20775.5 18 19682.0 19 19682.0 20 22710.5 21 25739.0 22 22070.0 23 18401.0 24 19455.0 25 20509.0 26 20509.0 27 22751.0 28 24993.0 29 27536.0 30 30079.0 31 33303.0 32 36527.0 33 36527.0 34 39550.0 35 42573.0 36 45502.5 37 48432.0 38 50951.0 39 53470.0 40 53470.0 41 54766.5 42 56063.0 43 56171.5 44 56280.0 45 56146.5 46 56013.0 47 56013.0 48 57053.5 49 58094.0 50 57654.0 51 57214.0 52 56836.5 53 56459.0 54 56459.0 55 56487.5 56 56516.0 57 53489.0 58 50462.0 59 46430.0 60 42398.0 61 42398.0 62 38359.0 63 34320.0 64 29214.5 65 24109.0 66 20586.0 67 17063.0 68 17063.0 69 14119.0 70 11175.0 71 9282.5 72 7390.0 73 5638.0 74 3886.0 75 3886.0 76 3005.5 77 2125.0 78 1704.0 79 1283.0 80 1045.5 81 808.0 82 808.0 83 656.5 84 505.0 85 424.0 86 343.0 87 287.5 88 232.0 89 232.0 90 192.0 91 152.0 92 83.5 93 15.0 94 13.0 95 11.0 96 11.0 97 7.5 98 4.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 960787.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.04907596735997 #Duplication Level Percentage of deduplicated Percentage of total 1 86.29012989029997 56.99383344357783 2 9.401797043404361 12.419600142990301 3 1.9894855950092751 3.942110556022079 4 0.7017684323535118 1.854046260000489 5 0.306282789583622 1.0114847618351792 6 0.2101104071068803 0.8326558944321161 7 0.14653377022416325 0.6774894084923601 8 0.11086562370966346 0.5858057602054646 9 0.088444248120957 0.5257494776715411 >10 0.6355192219563303 8.277741171491979 >50 0.07469645822143565 3.3979192825395943 >100 0.040735096932637535 4.688275274110533 >500 0.002052543478463075 0.8416724397631871 >1k 0.001105215719172425 1.3140768694654839 >5k 3.1577591976355E-4 1.525876736292275 >10k+ 1.57887959881775E-4 1.111662521109599 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10660 1.1095071019903475 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 7769 0.8086079432798321 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 6863 0.7143102477448174 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4957 0.5159312105596766 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1491 0.15518528040033847 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1392 0.14488122757697594 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1301 0.13540982548681446 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 1181 0.12292006448879927 TruSeq Adapter, Index 3 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 1157 0.12042211228919626 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT 1122 0.11677926533144184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0012489760998015168 0.0 3 0.0 0.0 0.0 0.0015612201247518962 0.0 4 0.0 0.0 0.0 0.0023938708579529073 0.0 5 0.0 0.0 0.0 0.0024979521996030337 0.0 6 0.0 0.0 0.0 0.0024979521996030337 0.0 7 0.0 0.0 0.0 0.003330602932804045 0.0 8 0.0 0.0 0.0 0.003330602932804045 0.0 9 0.0 0.0 0.0 0.004891823057555941 0.0 10 0.0 0.0 0.0 0.007389775257158975 0.0 11 0.0 0.0 0.0 0.00811834464870986 0.0 12 0.0 0.0 0.0 0.009159158065211124 0.0 13 0.0 0.0 0.0 0.009471402090161504 0.0 14 0.0 0.0 0.0 0.010512215506662767 0.0 15 0.0 0.0 0.0 0.011657110264814157 0.0 16 0.0 0.0 0.0 0.015508119905868834 0.0 17 0.0 0.0 0.0 0.022169325771476924 0.0 18 0.0 0.0 0.0 0.026228498095831856 0.0 19 0.0 0.0 0.0 0.030912158470087544 0.0 20 0.0 0.0 0.0 0.03476316811114222 0.0 21 0.0 0.0 0.0 0.04277743141820195 0.0 22 0.0 0.0 0.0 0.054850867049616614 0.0 23 0.0 0.0 0.0 0.0697344989055847 0.0 24 0.0 0.0 0.0 0.09981400664247123 0.0 25 0.0 0.0 0.0 0.1091813273909826 0.0 26 0.0 0.0 0.0 0.12125476302239727 0.0 27 0.0 0.0 0.0 0.13853226573631824 0.0 28 0.0 0.0 0.0 0.1694444242064058 0.0 29 0.0 0.0 0.0 0.21732184136546392 0.0 30 0.0 0.0 0.0 0.29340530211170635 0.0 31 0.0 0.0 0.0 0.5234250671584857 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACGG 140 0.0 23.785715 5 GCCGTCT 205 0.0 23.463415 36 ACGGACC 140 0.0 22.464287 8 CGGACCA 140 0.0 22.464287 9 CGCAAGA 175 0.0 19.02857 2 AGCGTCA 70 1.2189329E-4 18.5 3 TAGGCGA 50 0.0070340615 18.5 33 GCAGCGT 70 1.2189329E-4 18.5 1 GGTATCA 4200 0.0 18.32381 1 CCGTCTT 285 0.0 17.526316 37 GCGCTTT 95 3.6051424E-6 17.526316 13 TATGCCG 275 0.0 17.49091 33 TGCCGTC 275 0.0 17.49091 35 GACGGAC 180 0.0 17.472221 7 GCGCAAG 205 0.0 17.146343 1 ATGCCGT 290 0.0 16.586206 34 GCGAAAG 200 3.6379788E-12 15.725 18 GCGTTAT 85 5.3630135E-4 15.235294 1 GTTCCGG 85 5.3630135E-4 15.235294 26 CGAAAGC 210 9.094947E-12 14.97619 19 >>END_MODULE