FastQCFastQC Report
Fri 10 Feb 2017
ERR1630806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651214
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT165622.543249991554236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT146602.251180103621851No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124261.9081285107506902No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93521.4360870620103376No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT30750.4721950080925779No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25490.3914227888221077No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.378216684530738No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24490.3760668535995848No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT22560.34642989862011564RNA PCR Primer, Index 41 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.2797851397543665No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA17530.26918954445082566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT15130.23233529991677085No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA13500.20730512550405858No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC12380.19010647805483297No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG12040.18488546007917522No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC11740.18027867951241835No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA11690.1795108827512922No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.17643969570678764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11320.17382918671895875No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.17260071190115692No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10600.16277291335874228No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.1478776561928951No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9030.1386640950593814No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8980.13789629829825525No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8510.13067900874366953No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA7700.118240701213426No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC7680.11793358250897555No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA7250.11133053036329071No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC7060.10841290267101138No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6850.10518815627428156No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6770.10395968145647974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT3700.030.037
GTCTAAA250.005494615529.61
GCGCAGA250.005494615529.61
GCCGTCT3950.028.10126536
CGGTTAT400.001930293723.12514
TTACAAG400.001930293723.12522
GTTATTC400.001930293723.12516
TGGACTA502.7003593E-422.25
TCGGTTA502.7003593E-422.213
TTGGACT603.7230944E-521.5833324
TGCCGTC5300.020.94339635
GCAGTCG4350.020.8390799
AGCGTCA909.462383E-820.5555553
GGTTATT450.003824054320.55555515
TAATGTG450.003824054320.5555555
GGCAGTC4500.020.1444458
CAGTCGG4500.020.14444510
ATGCCGT5550.020.034
TTCAGGA656.8962596E-519.9230772
GCAGCGT851.2436121E-619.5882361
Produced by FastQC (version 0.11.5)