##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630806.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 651214 Sequences flagged as poor quality 0 Sequence length 43 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.825723955566065 33.0 31.0 34.0 30.0 34.0 2 31.895003792916 33.0 31.0 34.0 30.0 34.0 3 31.928350741845232 33.0 31.0 34.0 30.0 34.0 4 35.58991053632139 37.0 35.0 37.0 33.0 37.0 5 35.62646226893156 37.0 35.0 37.0 33.0 37.0 6 35.72400009827798 37.0 35.0 37.0 35.0 37.0 7 35.65899381770048 37.0 35.0 37.0 33.0 37.0 8 35.73139244549411 37.0 35.0 37.0 33.0 37.0 9 37.318729327072205 39.0 37.0 39.0 34.0 39.0 10 37.260565651229854 39.0 37.0 39.0 34.0 39.0 11 37.42082479799267 39.0 37.0 39.0 34.0 39.0 12 37.39165927022454 39.0 37.0 39.0 34.0 39.0 13 37.447149477744645 39.0 37.0 39.0 34.0 39.0 14 38.55291348158977 40.0 38.0 41.0 34.0 41.0 15 38.47575604947068 40.0 38.0 41.0 34.0 41.0 16 38.37382028027653 40.0 38.0 41.0 34.0 41.0 17 38.505540421428286 40.0 38.0 41.0 34.0 41.0 18 38.57544370974826 40.0 38.0 41.0 34.0 41.0 19 38.655283823750715 40.0 38.0 41.0 34.0 41.0 20 38.677896973959406 40.0 38.0 41.0 34.0 41.0 21 38.605165122371446 40.0 38.0 41.0 34.0 41.0 22 38.49328331393367 40.0 38.0 41.0 34.0 41.0 23 38.401083207670595 40.0 38.0 41.0 34.0 41.0 24 38.29247682021578 40.0 38.0 41.0 34.0 41.0 25 38.200355029222344 40.0 37.0 41.0 34.0 41.0 26 37.85413550691478 40.0 37.0 41.0 33.0 41.0 27 37.594028998148076 40.0 36.0 41.0 33.0 41.0 28 37.39391505710872 39.0 35.0 41.0 33.0 41.0 29 37.16509165957734 39.0 35.0 41.0 32.0 41.0 30 36.90096036018882 39.0 35.0 41.0 32.0 41.0 31 36.62696287241982 39.0 35.0 40.0 31.0 41.0 32 36.30911190484234 38.0 35.0 40.0 31.0 41.0 33 36.06113197812086 38.0 35.0 40.0 30.0 41.0 34 35.80225240857844 38.0 35.0 40.0 30.0 41.0 35 35.52184535344756 38.0 35.0 40.0 29.0 41.0 36 35.247330370661565 38.0 35.0 40.0 26.0 41.0 37 34.77823419029689 37.0 34.0 40.0 24.0 41.0 38 34.388832242550066 37.0 34.0 40.0 21.0 41.0 39 33.98896215376205 37.0 33.0 40.0 20.0 41.0 40 33.534844152613424 37.0 33.0 40.0 15.0 41.0 41 33.057019658668274 36.0 33.0 40.0 12.0 41.0 42 32.582037548332806 36.0 33.0 40.0 10.0 41.0 43 31.69116143080462 35.0 31.0 40.0 8.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 7.0 11 11.0 12 5.0 13 5.0 14 3.0 15 7.0 16 8.0 17 13.0 18 52.0 19 106.0 20 236.0 21 451.0 22 751.0 23 1322.0 24 2210.0 25 3364.0 26 5149.0 27 7366.0 28 10346.0 29 13311.0 30 16927.0 31 20149.0 32 24683.0 33 30507.0 34 37508.0 35 46524.0 36 62118.0 37 105607.0 38 124214.0 39 138253.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.201819985442576 20.470843685792996 13.448420949181067 17.87891537958336 2 17.98364285780097 24.61771399263529 33.23116517765282 24.167477971910923 3 24.03173150454382 28.364101508874196 28.97956739259291 18.62459959398907 4 13.866870183994816 17.257307121775636 30.998872874354667 37.87694981987488 5 11.533996504989142 43.22511493917514 30.487520231444655 14.753368324391062 6 30.77283350787913 31.34284582333918 18.16806763982347 19.71625302895822 7 24.768048598463793 35.80865890475328 21.767345296630598 17.65594720015233 8 31.955394079365618 29.601636328457314 20.2206955010181 18.222274091158972 9 28.606418166685604 12.037978298992345 23.509169028921367 35.84643450540068 10 20.847678336153706 30.656435518892405 27.82679733543812 20.669088809515763 11 28.502612044581348 25.268037849309138 20.081417168549816 26.1479329375597 12 20.814970194129735 32.60971047919731 27.56236813090628 19.012951195766675 13 29.262730838096235 20.976361073318447 30.347781220919696 19.41312686766562 14 22.874661785526722 24.304913592152502 30.56783177265845 22.25259284966232 15 27.143765336740305 25.637655210115263 28.876221948545332 18.342357504599104 16 21.438267604811937 26.276769234076664 31.688968603254846 20.595994557856557 17 18.94968474264988 30.324747318085915 30.26363069590027 20.46193724336393 18 18.130292039176062 24.214006455635168 34.88162109536957 22.7740804098192 19 18.20492188435752 27.861348189688794 36.394794952196975 17.538934973756707 20 19.480539423292498 24.42699327717156 37.62280909194213 18.469658207593817 21 21.547448304244075 25.604639949386836 35.45685442880528 17.391057317563813 22 21.21806349372096 26.63440896540922 34.362744044200525 17.7847834966693 23 19.362759400135747 26.82267273123735 34.97636721569254 18.838200652934365 24 17.839604185413705 28.867929743525174 34.97621365634031 18.316252414720815 25 17.4342074955391 28.854723639233796 35.24248557309886 18.46858329212824 26 18.55549788548772 28.703621236644178 34.83877803609873 17.90210284176937 27 17.72259195901808 30.000737084890684 34.5470152668708 17.72965568922044 28 16.901049424613106 29.174280651214502 35.99108741519685 17.933582508975544 29 16.83471178445181 29.834739425135208 35.823861280623575 17.50668750978941 30 15.520857966812754 31.08701594253195 36.16660575479028 17.225520335865017 31 17.062593863154046 30.873107764882207 34.53672679027171 17.52757158169204 32 15.74843292681054 31.6905041967771 34.99510145666401 17.565961419748348 33 15.302803686652927 31.383078373622187 35.308669653907934 18.005448285816954 34 16.980286050361325 31.213088170708858 34.235904019262485 17.57072175966733 35 15.531607121468518 31.952783570377786 34.82910379690855 17.68650551124515 36 16.009944503650107 31.728126238072278 33.228247549960535 19.033681708317083 37 16.124346221057902 31.185293927956092 34.09017619400074 18.600183656985262 38 15.967562122435943 30.291885616709713 34.130715862988204 19.60983639786614 39 16.433154078382834 30.905201669497277 34.02629550347505 18.63534874864484 40 15.393864382522487 30.716937903669145 34.6486715580439 19.240526155764464 41 15.47786134818969 30.99011999127783 33.650228649875466 19.88179001065702 42 15.483082366165346 31.046322714192264 34.278286400476645 19.192308519165742 43 14.764271038399052 30.429321236951296 33.66450966963241 21.141898055017244 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 670.0 1 546.0 2 422.0 3 1421.0 4 2420.0 5 2420.0 6 3666.5 7 4913.0 8 4988.5 9 5064.0 10 7435.5 11 9807.0 12 9807.0 13 17510.5 14 25214.0 15 32852.0 16 40490.0 17 36140.0 18 31790.0 19 31790.0 20 33588.0 21 35386.0 22 25847.0 23 16308.0 24 15065.5 25 13823.0 26 13823.0 27 14054.5 28 14286.0 29 14649.0 30 15012.0 31 15678.5 32 16345.0 33 16345.0 34 17564.5 35 18784.0 36 19375.5 37 19967.0 38 22884.5 39 25802.0 40 25802.0 41 28188.0 42 30574.0 43 32196.5 44 33819.0 45 35877.0 46 37935.0 47 37935.0 48 40408.5 49 42882.0 50 41826.5 51 40771.0 52 36358.0 53 31945.0 54 31945.0 55 30917.0 56 29889.0 57 27010.0 58 24131.0 59 21546.0 60 18961.0 61 18961.0 62 18101.5 63 17242.0 64 15559.5 65 13877.0 66 12929.0 67 11981.0 68 11981.0 69 9832.0 70 7683.0 71 6678.0 72 5673.0 73 4271.0 74 2869.0 75 2869.0 76 2291.0 77 1713.0 78 1315.5 79 918.0 80 757.5 81 597.0 82 597.0 83 511.5 84 426.0 85 396.0 86 366.0 87 300.5 88 235.0 89 235.0 90 208.5 91 182.0 92 109.0 93 36.0 94 20.0 95 4.0 96 4.0 97 3.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 651214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.03643166101566 #Duplication Level Percentage of deduplicated Percentage of total 1 80.3995373575015 32.18910582990885 2 9.907930256160276 7.933563452057406 3 3.1990117741970012 3.8423104884126813 4 1.553835433638903 2.4884010460539434 5 0.944134664930891 1.8899891495650771 6 0.6278064258286442 1.508107743842101 7 0.4614095214086172 1.2931233540132632 8 0.3590575387031704 1.1500306088529577 9 0.2633743092077592 0.9490110778677294 >10 1.922664368289098 15.09090304304359 >50 0.1959910198088651 5.398277659001247 >100 0.14795400505868195 11.27120863815362 >500 0.009223106808852901 2.4595812180340957 >1k 0.006533033989604138 4.381888720731331 >5k 3.842961170355375E-4 1.4388842456558781 >10k+ 0.0011528883511066127 6.71561372480622 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 16562 2.543249991554236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 14660 2.251180103621851 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 12426 1.9081285107506902 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9352 1.4360870620103376 No Hit CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT 3075 0.4721950080925779 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2549 0.3914227888221077 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2463 0.378216684530738 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2449 0.3760668535995848 No Hit TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT 2256 0.34642989862011564 RNA PCR Primer, Index 41 (95% over 22bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1822 0.2797851397543665 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 1753 0.26918954445082566 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 1513 0.23233529991677085 No Hit GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 1350 0.20730512550405858 No Hit CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC 1238 0.19010647805483297 No Hit ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG 1204 0.18488546007917522 No Hit ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC 1174 0.18027867951241835 No Hit ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 1169 0.1795108827512922 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1149 0.17643969570678764 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1132 0.17382918671895875 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1124 0.17260071190115692 No Hit GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1060 0.16277291335874228 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 963 0.1478776561928951 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA 903 0.1386640950593814 No Hit GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 898 0.13789629829825525 No Hit GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA 851 0.13067900874366953 No Hit TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA 770 0.118240701213426 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC 768 0.11793358250897555 No Hit GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA 725 0.11133053036329071 No Hit CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC 706 0.10841290267101138 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 685 0.10518815627428156 No Hit GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA 677 0.10395968145647974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.0711870445045716E-4 0.0 2 0.0 0.0 0.0 0.0032247463967298 0.0 3 0.0 0.0 0.0 0.004453221214531629 0.0 4 0.0 0.0 0.0 0.006603052145684829 0.0 5 0.0 0.0 0.0 0.007985086315711886 0.0 6 0.0 0.0 0.0 0.008138645667937114 0.0 7 0.0 0.0 0.0 0.008906442429063258 0.0 8 0.0 0.0 0.0 0.00982779854241463 0.0 9 0.0 0.0 0.0 0.0139739010524958 0.0 10 0.0 0.0 0.0 0.019809156437054484 0.0 11 0.0 0.0 0.0 0.02487661506048703 0.0 12 0.0 0.0 0.0 0.029483395627243884 0.0 13 0.0 0.0 0.0 0.031633226558397086 0.0 14 0.0 0.0 0.0 0.03777560064740623 0.0 15 0.0 0.0 0.0 0.04637492437201903 0.0 16 1.5355935222522858E-4 0.0 0.0 0.06495560599127169 0.0 17 1.5355935222522858E-4 0.0 0.0 0.0902928991084344 0.0 18 1.5355935222522858E-4 0.0 0.0 0.09919934153749765 0.0 19 1.5355935222522858E-4 0.0 0.0 0.11639798898672325 0.0 20 1.5355935222522858E-4 0.0 0.0 0.12760782169916493 0.0 21 1.5355935222522858E-4 0.0 0.0 0.15494138639525562 0.0 22 1.5355935222522858E-4 0.0 0.0 0.21022275319633793 0.0 23 1.5355935222522858E-4 0.0 0.0 0.295601753033565 0.0 24 1.5355935222522858E-4 0.0 0.0 0.4262807617772345 0.0 25 1.5355935222522858E-4 0.0 0.0 0.4586817850967577 0.0 26 1.5355935222522858E-4 0.0 0.0 0.5131953551367139 0.0 27 1.5355935222522858E-4 0.0 0.0 0.569705196755598 0.0 28 1.5355935222522858E-4 0.0 0.0 0.6527808063094467 0.0 29 1.5355935222522858E-4 0.0 0.0 0.7788530344863593 0.0 30 1.5355935222522858E-4 0.0 0.0 0.981551379423661 0.0 31 1.5355935222522858E-4 0.0 0.0 1.5286833514021505 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCTT 370 0.0 30.0 37 GTCTAAA 25 0.0054946155 29.6 1 GCGCAGA 25 0.0054946155 29.6 1 GCCGTCT 395 0.0 28.101265 36 CGGTTAT 40 0.0019302937 23.125 14 TTACAAG 40 0.0019302937 23.125 22 GTTATTC 40 0.0019302937 23.125 16 TGGACTA 50 2.7003593E-4 22.2 5 TCGGTTA 50 2.7003593E-4 22.2 13 TTGGACT 60 3.7230944E-5 21.583332 4 TGCCGTC 530 0.0 20.943396 35 GCAGTCG 435 0.0 20.839079 9 AGCGTCA 90 9.462383E-8 20.555555 3 GGTTATT 45 0.0038240543 20.555555 15 TAATGTG 45 0.0038240543 20.555555 5 GGCAGTC 450 0.0 20.144445 8 CAGTCGG 450 0.0 20.144445 10 ATGCCGT 555 0.0 20.0 34 TTCAGGA 65 6.8962596E-5 19.923077 2 GCAGCGT 85 1.2436121E-6 19.588236 1 >>END_MODULE