FastQCFastQC Report
Fri 10 Feb 2017
ERR1630805.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630805.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565733
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76610.48929159697087565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75700.4834796226431965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59240.37835314194693476No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA58760.3752874851587084No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC53920.3443754458774261No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC53310.34047950704238844No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA45780.2923870161770876No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC44630.2850422134552954No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC39510.25234187438088107No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC35130.22436775618831561No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC35030.22372907769076847No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG33150.21172192193688197No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC32570.20801758665110848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32010.2044409870648444No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC31860.20348296931852364No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT30180.19275317055973143No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG29220.18662185698327874No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG27870.17799969726639217No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC27830.1777442258673733No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG27770.177361018768845No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC27380.1748701726284111No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC26050.16637574861103394No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT25820.16490678806667547No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT24820.15852000309120393No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG24170.1543685928571474No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT23780.1518777467167135No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG23490.15002557907382677No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG23090.14747086508363816No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC22740.1452354903422231No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG22010.14057313731012888No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG21520.1374436126721478No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA21420.13680493417460066No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT21010.1341863523346573No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC20800.13284512748980828No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG20690.13214258114250643No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG20510.13099295984692155No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC20380.13016267780011023No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC20340.12990720640109138No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC19800.12645834251433674No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC19520.12467004272120472No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC19510.12460617487144998No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG19380.12377589282463869No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC19360.12364815712512926No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG18710.11949674689107274No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA18170.1160478830043181No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC17780.11355703686388419No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG17620.11253515126780876No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG17400.111130058573205No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC17370.11093845502394087No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC16920.10806440178497867No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT16900.10793666608546924No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG16810.10736185543767679No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG16780.10717025188841266No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT16650.10633996984160134No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC16610.10608449844258247No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC16000.10218855960754483No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT15800.10091120261245053No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC15750.10059186336367695No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG15740.10052799551392222No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC15740.10052799551392222No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG15680.10014478841539394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAA250.005496945729.59999822
GCCGTCT1500.028.36666736
GGTATCA32500.023.110771
TATGCCG1850.023.00000233
CCGTCTT2050.021.65853537
GGTATTG1400.021.14285933
CGGTCCA909.483847E-820.55555510
CCGTTGC450.003826417720.55555532
TTAGGTT1650.020.1818184
CTTTCGG656.904172E-519.92307721
AATACTG4500.019.7333345
AGGCACT1601.8189894E-1218.536
GCAATAC902.1532287E-618.537
CTAGCGG902.1532287E-618.529
CGACAAC500.00703599518.49999824
GCATTCG500.00703599518.49999822
CTCTAAT5500.017.8272721
ATACTGG5950.017.7226896
TGTGCTA953.6080764E-617.52631626
TCTAGCG953.6080764E-617.52631628
Produced by FastQC (version 0.11.5)