FastQCFastQC Report
Fri 10 Feb 2017
ERR1630804.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630804.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803211
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51170.28377156084340655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43480.24112541460760833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39550.21933096015940454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29430.1632088535396024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT500.00703639718.528
CGAATTA1205.178663E-918.515
GGTATCA22700.018.0110131
GTATAGA1603.45608E-1117.343751
GCTATAC1401.87174E-917.1785723
GTATTAG3800.016.5526311
TAACGGC1251.6591366E-716.27999936
TTAACGG1251.6591366E-716.27999935
CACCGAT803.383901E-416.187526
GACGGAC2900.015.94827657
GTAATAC1455.3503754E-815.3103463
ACGGACC2950.015.0508478
TAGACAG1853.0559022E-1015.05
CGTTATT2109.094947E-1214.97619152
CGCAAGA3250.014.8000012
ATAACGC2006.184564E-1114.83
TAGTACC1752.240995E-914.84
GTACTAG750.004105611214.7999991
CAAGACG3600.014.3888894
AACGCCG1956.730261E-1014.230775
Produced by FastQC (version 0.11.5)