FastQCFastQC Report
Fri 10 Feb 2017
ERR1630803.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630803.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107402
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30552.844453548351055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24142.2476303979441727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16081.4971788234855963No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT14751.3733450028863523No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT14491.3491368875812368TruSeq Adapter, Index 10 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11021.0260516563937359No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC8220.765348876184801No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC7420.6908623675536768No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG6690.6228934284277761No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC3820.35567307871361803RNA PCR Primer, Index 10 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.31749874304016684No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3200.29794603452449675No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.2811865700824938No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.230908176756485No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG2380.22159736317759446No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2290.21321763095659296No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2080.1936649224409229No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2030.1890095156514776No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1600.14897301726224838No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.14338652911491406No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1540.14338652911491406No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1520.14152436639913596No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.14152436639913596No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.13966220368335786No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.13407571553602354No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1300.12104057652557679No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1260.1173162510940206No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1240.1154540883782425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1160.10800543751513006No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1090.1014878680099067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC2300.029.760873
GACAGAG250.005474460329.5999987
TTATACA2350.029.127662
CTTATAC2300.028.9565221
CGTCTTC508.991314E-625.89999837
CCGTCTT1800.023.6388937
GCCGTCT1850.023.036
ATACACA3150.021.730164
TACACAT3300.020.7424245
TCTTATA1251.8189894E-1120.7237
TTCCAGA500.006994449518.49999819
AGCTGAT500.006994449518.4999987
ACACATC4150.016.4939776
AGCAGTG904.3858978E-516.44444525
CACATCT4500.015.2111117
GGTATCA16200.015.18827151
CAGTCGG1752.1518645E-914.79999910
GATTCCT1752.1518645E-914.79999918
AGTCGGT1752.1518645E-914.79999911
ACATCTC4650.014.7204298
Produced by FastQC (version 0.11.5)