##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630803.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	107402
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49462766056498	31.0	31.0	34.0	30.0	34.0
2	31.59794975884993	31.0	31.0	34.0	30.0	34.0
3	31.544282229381203	31.0	31.0	34.0	28.0	34.0
4	35.32843894899536	37.0	35.0	37.0	33.0	37.0
5	35.35799147129476	37.0	35.0	37.0	33.0	37.0
6	35.41408912310758	37.0	35.0	37.0	33.0	37.0
7	35.39027206197277	37.0	35.0	37.0	33.0	37.0
8	35.44362302377982	37.0	35.0	37.0	33.0	37.0
9	36.9894136049608	39.0	37.0	39.0	33.0	39.0
10	36.876026517197076	39.0	37.0	39.0	33.0	39.0
11	37.057773598257015	39.0	37.0	39.0	33.0	39.0
12	36.98025176439917	39.0	37.0	39.0	33.0	39.0
13	37.06253142399583	39.0	37.0	39.0	33.0	39.0
14	38.04261559375058	40.0	37.0	41.0	33.0	41.0
15	37.99995344593211	40.0	37.0	41.0	33.0	41.0
16	37.78604681477067	39.0	37.0	41.0	32.0	41.0
17	37.91849313793039	39.0	37.0	41.0	33.0	41.0
18	38.02972011694382	40.0	37.0	41.0	33.0	41.0
19	38.07280125137334	40.0	37.0	41.0	33.0	41.0
20	38.12454144243124	40.0	38.0	41.0	33.0	41.0
21	38.042029012495114	40.0	37.0	41.0	33.0	41.0
22	37.91905178674513	40.0	37.0	41.0	33.0	41.0
23	37.84861548202082	40.0	37.0	41.0	33.0	41.0
24	37.730424014450385	40.0	37.0	41.0	33.0	41.0
25	37.643656542708705	40.0	37.0	41.0	32.0	41.0
26	37.283020800357534	39.0	36.0	41.0	32.0	41.0
27	36.974330086963	39.0	35.0	41.0	31.0	41.0
28	36.84416491313011	39.0	35.0	40.0	31.0	41.0
29	36.55900262564943	39.0	35.0	40.0	30.0	41.0
30	36.342451723431594	38.0	35.0	40.0	30.0	41.0
31	36.058034301037225	38.0	35.0	40.0	30.0	41.0
32	35.73466974544235	38.0	35.0	40.0	29.0	41.0
33	35.49984171616916	38.0	35.0	40.0	28.0	41.0
34	35.275134541256215	37.0	34.0	40.0	27.0	41.0
35	35.038881957505446	37.0	34.0	40.0	26.0	41.0
36	34.80139103554868	37.0	34.0	40.0	25.0	41.0
37	34.28986424833802	37.0	33.0	40.0	23.0	41.0
38	33.99087540269269	36.0	33.0	40.0	22.0	41.0
39	33.6532280590678	36.0	33.0	40.0	21.0	41.0
40	33.20583415578853	35.0	33.0	40.0	18.0	41.0
41	32.773542392134225	35.0	32.0	39.0	15.0	41.0
42	32.34513323774232	35.0	31.0	39.0	12.0	41.0
43	31.23842200331465	35.0	29.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	0.0
14	0.0
15	1.0
16	2.0
17	7.0
18	11.0
19	29.0
20	62.0
21	97.0
22	204.0
23	287.0
24	451.0
25	734.0
26	1025.0
27	1430.0
28	2048.0
29	2609.0
30	3065.0
31	3793.0
32	4498.0
33	5565.0
34	6915.0
35	8672.0
36	12103.0
37	18165.0
38	22076.0
39	13549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.049440420103906	19.349732779650285	14.841436844751493	20.759389955494314
2	19.11975568425169	23.23979069291075	31.721010781922125	25.91944284091544
3	23.27610286586842	24.451127539524403	31.895122995847373	20.3776465987598
4	14.124504199176924	18.534105510139476	27.902646133219122	39.43874415746448
5	13.02675927822573	39.747863168283644	30.644680732202378	16.580696821288242
6	31.381166086292623	28.92683562689708	17.770618796670455	21.92137949013985
7	25.333792666803223	35.120388819575055	18.750116385169736	20.795702128451985
8	33.806633023593605	28.920318057391853	19.152343531777806	18.12070538723674
9	27.233198636896894	11.388987169698888	20.45306418874881	40.92475000465541
10	22.361780972421368	27.954786689260906	25.094504757825735	24.588927580491983
11	30.59812666430793	22.66438241373531	18.376752760656228	28.360738161300535
12	20.30036684605501	31.270367404703826	25.319826446434888	23.10943930280628
13	33.44630453809054	17.049961825664326	27.82536638051433	21.67836725573081
14	23.807750321223068	22.896221671849688	27.69594607176775	25.600081935159498
15	31.531070184912757	22.365505297852923	24.08148824044245	22.021936276791866
16	25.25278858866688	22.435336399694606	29.320683041284145	22.991191970354368
17	19.411184149270962	31.35230256419806	27.192231057149773	22.044282229381203
18	21.33200499059608	20.058285693003857	31.241503882609262	27.368205433790806
19	21.029403549282137	26.358913241839073	32.504981285264705	20.106701923614086
20	22.622483752630306	20.69980074858941	34.98351985996536	21.69419563881492
21	25.47531703320236	21.707230777825366	32.02175006052029	20.795702128451985
22	25.392450792350235	23.574048900392917	30.93890244129532	20.09459786596153
23	23.19696095044785	23.152269045269176	31.205191709651594	22.445578294631385
24	22.02845384629709	24.25746261708348	32.43328802070725	21.280795515912178
25	20.944675145714235	25.323550771866444	33.06828550678758	20.66348857563174
26	23.779817880486398	24.07310850822145	31.599039124038658	20.548034487253496
27	20.89439675238822	26.366361892702184	32.0971676505093	20.64207370440029
28	20.274296568034114	24.763039794417235	33.637176216457796	21.325487421090855
29	19.24824491164038	25.476248114560253	32.859723282620436	22.415783691178937
30	20.17187761866632	26.00417124448334	35.87177147539152	17.952179661458818
31	19.43539226457608	27.371929759222365	31.276884974209047	21.915793001992515
32	19.788272099216027	27.36168786428558	34.08316418688665	18.76687584961174
33	19.683059905774567	25.977169885104562	34.412766987579374	19.927003221541497
34	18.467998733729356	28.505055771773335	31.17353494348336	21.853410551013948
35	18.316232472393438	27.16709186048677	35.344779426826314	19.171896240293478
36	20.169084374592654	28.05999888270237	30.268523863615204	21.502392879089776
37	18.49872441853969	28.842107223329172	31.454721513565854	21.20444684456528
38	18.830189381948195	26.499506526880317	32.59995158376939	22.070352507402095
39	20.51637772108527	26.900802592130503	30.877451071674646	21.705368615109588
40	17.956835068248264	26.62799575426901	34.07199121059198	21.343177966890746
41	18.332060855477554	27.97527047913447	30.354183348540996	23.338485316846985
42	18.182156756857417	26.952943148172288	33.00404089309324	21.86085920187706
43	17.178451053053013	28.014375896165806	32.24055417962421	22.56661887115696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	16.5
2	28.0
3	118.5
4	209.0
5	209.0
6	333.5
7	458.0
8	516.0
9	574.0
10	795.0
11	1016.0
12	1016.0
13	1932.5
14	2849.0
15	4202.5
16	5556.0
17	4844.0
18	4132.0
19	4132.0
20	4873.5
21	5615.0
22	3927.0
23	2239.0
24	1969.5
25	1700.0
26	1700.0
27	1809.5
28	1919.0
29	1922.5
30	1926.0
31	1954.5
32	1983.0
33	1983.0
34	2022.5
35	2062.0
36	2017.5
37	1973.0
38	2219.0
39	2465.0
40	2465.0
41	2848.5
42	3232.0
43	3636.0
44	4040.0
45	4686.5
46	5333.0
47	5333.0
48	6581.5
49	7830.0
50	7362.5
51	6895.0
52	6085.0
53	5275.0
54	5275.0
55	6144.0
56	7013.0
57	5877.0
58	4741.0
59	5559.0
60	6377.0
61	6377.0
62	5951.5
63	5526.0
64	4567.5
65	3609.0
66	3417.5
67	3226.0
68	3226.0
69	2992.5
70	2759.0
71	2337.0
72	1915.0
73	1621.5
74	1328.0
75	1328.0
76	1064.5
77	801.0
78	611.0
79	421.0
80	310.0
81	199.0
82	199.0
83	155.0
84	111.0
85	78.5
86	46.0
87	27.0
88	8.0
89	8.0
90	7.0
91	6.0
92	3.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	107402.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.94867879555315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.24696288899983	47.135062661775386
2	9.251123315027458	10.351762537010483
3	2.527874854385089	4.242937747900412
4	1.164919287735064	2.6070278020893465
5	0.619071392910634	1.7318113256736374
6	0.42769179563987353	1.4357274538649187
7	0.291229821933766	1.140574663414089
8	0.21467798302546182	0.960875961341502
9	0.16475287069395905	0.8295934898791456
>10	0.963554667998003	10.200927357032457
>50	0.07322349808620403	2.8044170499618257
>100	0.03994008986520219	4.142380961248394
>500	0.004992511233150274	2.079104672166254
>1k	0.009985022466300548	10.337796316642148
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3055	2.844453548351055	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2414	2.2476303979441727	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1608	1.4971788234855963	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	1475	1.3733450028863523	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	1449	1.3491368875812368	TruSeq Adapter, Index 10 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1102	1.0260516563937359	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	822	0.765348876184801	No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	742	0.6908623675536768	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	669	0.6228934284277761	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	382	0.35567307871361803	RNA PCR Primer, Index 10 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	341	0.31749874304016684	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	320	0.29794603452449675	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.2811865700824938	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	248	0.230908176756485	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	238	0.22159736317759446	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	229	0.21321763095659296	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	208	0.1936649224409229	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	203	0.1890095156514776	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	160	0.14897301726224838	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	154	0.14338652911491406	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	154	0.14338652911491406	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	152	0.14152436639913596	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	152	0.14152436639913596	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	150	0.13966220368335786	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	144	0.13407571553602354	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	130	0.12104057652557679	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	126	0.1173162510940206	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	124	0.1154540883782425	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	116	0.10800543751513006	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	109	0.1014878680099067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0018621627157781046	0.0
3	0.0	0.0	0.0	0.0037243254315562093	0.0
4	0.0	0.0	0.0	0.0037243254315562093	0.0
5	0.0	0.0	0.0	0.0037243254315562093	0.0
6	0.0	0.0	0.0	0.0037243254315562093	0.0
7	0.0	0.0	0.0	0.0037243254315562093	0.0
8	0.0	0.0	0.0	0.0037243254315562093	0.0
9	0.0	0.0	0.0	0.005586488147334314	0.0
10	0.0	0.0	0.0	0.006517569505223367	0.0
11	0.0	0.0	0.0	0.008379732221001471	0.0
12	0.0	0.0	0.0	0.008379732221001471	0.0
13	0.0	0.0	0.0	0.009310813578890523	0.0
14	0.0	0.0	0.0	0.012104057652557681	0.0
15	0.0	0.0	0.0	0.01582838308411389	0.0
16	0.0	0.0	0.0	0.032587847526116834	0.0
17	0.0	0.0	0.0	0.04934731196811978	0.0
18	0.0	0.0	0.0	0.05493380011545409	0.0
19	0.0	0.0	0.0	0.07355542727323514	0.0
20	0.0	0.0	0.0	0.07914191542056945	0.0
21	0.0	0.0	0.0	0.09310813578890524	0.0
22	0.0	0.0	0.0	0.12010949516768775	0.0
23	0.0	0.0	0.0	0.15921491219902795	0.0
24	0.0	0.0	0.0	0.23183925811437403	0.0
25	0.0	0.0	0.0	0.2523230479879332	0.0
26	0.0	0.0	0.0	0.2877041395877172	0.0
27	0.0	0.0	0.0	0.3147054989664997	0.0
28	0.0	0.0	0.0	0.3510176719241727	0.0
29	0.0	0.0	0.0	0.4106068788290721	0.0
30	0.0	0.0	0.0	0.515819072270535	0.0
31	0.0	0.0	0.0	0.8454218729632595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	230	0.0	29.76087	3
GACAGAG	25	0.0054744603	29.599998	7
TTATACA	235	0.0	29.12766	2
CTTATAC	230	0.0	28.956522	1
CGTCTTC	50	8.991314E-6	25.899998	37
CCGTCTT	180	0.0	23.63889	37
GCCGTCT	185	0.0	23.0	36
ATACACA	315	0.0	21.73016	4
TACACAT	330	0.0	20.742424	5
TCTTATA	125	1.8189894E-11	20.72	37
TTCCAGA	50	0.0069944495	18.499998	19
AGCTGAT	50	0.0069944495	18.499998	7
ACACATC	415	0.0	16.493977	6
AGCAGTG	90	4.3858978E-5	16.444445	25
CACATCT	450	0.0	15.211111	7
GGTATCA	1620	0.0	15.1882715	1
CAGTCGG	175	2.1518645E-9	14.799999	10
GATTCCT	175	2.1518645E-9	14.799999	18
AGTCGGT	175	2.1518645E-9	14.799999	11
ACATCTC	465	0.0	14.720429	8
>>END_MODULE