FastQCFastQC Report
Fri 10 Feb 2017
ERR1630802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972573
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71420.36206518085769196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62660.31765617799696133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57500.29149745028447616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39460.20004329370826834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33050.018.6959151
AAGACGG4050.017.3580255
CAAGACG4800.015.8020834
GAGTACG5500.015.4727281
TCTAGCG2850.014.92982428
CGCAAGA4750.014.82
CTACGGA750.00410579314.7999994
GCGCAAG4700.014.5638281
CTAGATA3450.014.478263
GTCTAGC908.277721E-414.3888881
CGAATTA1551.2113924E-714.32258115
GACGGAC4800.014.2604167
TACGACG3250.014.2307695
ATCTCGT3150.014.09523937
CTAGCGG3150.014.09523929
ACTAGCG3550.014.07042227
AACGCTA800.006300729713.87500133
GGACCGT1203.3027158E-513.8749996
AGACGGA5350.013.8317766
ACGGACC5000.013.698
Produced by FastQC (version 0.11.5)