##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630802.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1972573
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96893904560186	33.0	31.0	34.0	30.0	34.0
2	32.13336084393328	33.0	31.0	34.0	30.0	34.0
3	32.23756231074845	34.0	31.0	34.0	30.0	34.0
4	35.82551469578059	37.0	35.0	37.0	35.0	37.0
5	35.80496184425114	37.0	35.0	37.0	35.0	37.0
6	35.839562845075946	37.0	35.0	37.0	35.0	37.0
7	35.810730959006335	37.0	35.0	37.0	35.0	37.0
8	35.802452938370344	37.0	35.0	37.0	35.0	37.0
9	37.53088073293105	39.0	37.0	39.0	35.0	39.0
10	37.43263139057465	39.0	37.0	39.0	34.0	39.0
11	37.54361536936783	39.0	37.0	39.0	35.0	39.0
12	37.46718068228654	39.0	37.0	39.0	35.0	39.0
13	37.514777906825245	39.0	37.0	39.0	35.0	39.0
14	38.77842391637724	40.0	38.0	41.0	35.0	41.0
15	38.75137092518249	40.0	38.0	41.0	35.0	41.0
16	38.731646940316026	40.0	38.0	41.0	35.0	41.0
17	38.719671211154164	40.0	38.0	41.0	35.0	41.0
18	38.70662530613569	40.0	38.0	41.0	35.0	41.0
19	38.74448854364325	40.0	38.0	41.0	34.0	41.0
20	38.72400210283726	40.0	38.0	41.0	34.0	41.0
21	38.6481275978126	40.0	38.0	41.0	34.0	41.0
22	38.58841523228798	40.0	38.0	41.0	34.0	41.0
23	38.55520125237444	40.0	38.0	41.0	34.0	41.0
24	38.503344109444875	40.0	38.0	41.0	34.0	41.0
25	38.45975890372625	40.0	38.0	41.0	34.0	41.0
26	38.26775282841243	40.0	38.0	41.0	34.0	41.0
27	38.15258649489778	40.0	38.0	41.0	33.0	41.0
28	38.0505750611004	40.0	37.0	41.0	33.0	41.0
29	37.94494196159027	40.0	37.0	41.0	33.0	41.0
30	37.866769442753196	40.0	37.0	41.0	33.0	41.0
31	37.81922848989619	40.0	37.0	41.0	33.0	41.0
32	37.716580324276975	40.0	37.0	41.0	33.0	41.0
33	37.66840973692735	40.0	37.0	41.0	33.0	41.0
34	37.59534830903596	40.0	37.0	41.0	32.0	41.0
35	37.53108402071812	40.0	37.0	41.0	32.0	41.0
36	37.480841013235	40.0	37.0	41.0	32.0	41.0
37	37.300712318378075	40.0	36.0	41.0	31.0	41.0
38	37.17489694931442	40.0	36.0	41.0	31.0	41.0
39	37.11391365490656	39.0	36.0	41.0	31.0	41.0
40	37.008350514784496	39.0	36.0	41.0	31.0	41.0
41	36.87744585371492	39.0	35.0	41.0	31.0	41.0
42	36.77633071120815	39.0	35.0	41.0	30.0	41.0
43	35.915540768326444	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	7.0
12	2.0
13	3.0
14	3.0
15	4.0
16	10.0
17	35.0
18	76.0
19	157.0
20	299.0
21	635.0
22	1058.0
23	1966.0
24	3137.0
25	4817.0
26	7460.0
27	11229.0
28	15735.0
29	22243.0
30	29821.0
31	38937.0
32	50637.0
33	65185.0
34	85681.0
35	114459.0
36	160426.0
37	242574.0
38	433326.0
39	682643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23368514118362	19.545841902935912	12.294602024868027	24.92587093101244
2	18.829011651279824	21.50399503592516	34.272952129021334	25.39404118377368
3	20.680552760278072	23.768195144108738	29.293719421283775	26.257532674329415
4	14.687872134516693	15.548271217338977	34.38149057094465	35.38236607719968
5	14.393992009421197	37.17281945966005	33.92705871975334	14.506129811165417
6	34.66340662677629	34.55714946924651	15.759264676136194	15.020179227840996
7	28.418111775837957	30.80514637481097	21.738308290745135	19.038433558605945
8	27.154635088283168	32.82971023125633	20.485832463488045	19.529822216972452
9	26.55328852214848	14.021331529935773	20.50124380694656	38.92413614096918
10	17.37492097884337	26.602767045883724	32.683353163609155	23.33895881166375
11	35.19535145213891	22.049526177231463	21.587642130354617	21.16748024027501
12	21.55757987156876	26.057641466247382	28.796044557032868	23.588734105150987
13	29.551453862543998	20.02536788245606	25.818410776179135	24.60476747882081
14	22.888734662798285	21.669616282895486	25.29726403027923	30.14438502402699
15	25.63524898698299	27.80981996610518	22.934613826712624	23.6203172201992
16	25.148625678238524	26.57037280749559	24.467484853539005	23.813516660726876
17	23.43507692744451	26.589028644313796	25.740441545129126	24.235452883112565
18	23.101958710780284	24.83690083966474	27.805257397318123	24.25588305223685
19	23.70801993132827	25.698313826661927	27.395589415448757	23.198076826561046
20	24.381607169924763	24.7239519145806	27.338202439149274	23.556238476345364
21	24.81175601612716	25.559915906787733	26.342345758560015	23.285982318525093
22	24.43686494745695	25.263348935628745	26.49062924413951	23.809156872774796
23	23.628935405685873	25.40382535906149	27.024500487434434	23.942738747818204
24	23.995918021791844	25.917215738023387	26.636276578864255	23.45058966132052
25	24.183135427687592	25.241296519824612	26.728744639615364	23.846823412872425
26	23.774734826036855	26.16942440153039	26.615136676817535	23.44070409561522
27	24.37496609757915	25.238812454596104	26.34001377895774	24.04620766886701
28	23.229507855983023	25.717375225150096	27.325376551336756	23.727740367530124
29	23.07351869867427	26.048567023882008	27.254758125554794	23.62315615188893
30	22.850155608943243	26.78060583816163	27.174659695737496	23.19457885715763
31	23.744621872042252	26.27978787096853	26.420568465653744	23.555021791335477
32	23.011619848796467	25.953919069154857	26.58264104801191	24.451820034036764
33	22.914944085719515	25.661813276365436	27.3030706594889	24.120171978426146
34	23.693216930374696	25.48351822720883	26.891577650104708	23.931687192311767
35	23.614233795149786	25.536089158677523	27.5287657288222	23.320911317350486
36	22.83220950504747	26.191780988586988	27.021458774909725	23.95455073145582
37	23.749336526455547	25.16783916235293	27.064245531090613	24.018578780100913
38	22.856593900453877	25.291079214812328	27.94771093389193	23.90461595084187
39	23.189458641074374	25.101073572435595	27.652614123786545	24.056853662703485
40	22.75636947276476	25.106954216650028	28.394690589397705	23.741985721187504
41	22.074671000769047	25.285604132267853	28.71346206198706	23.92626280497604
42	22.88047134377283	25.09417902404626	28.530148187164684	23.49520144501623
43	22.095405341145803	24.58448939532276	28.49243095185831	24.827674311673128
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	336.0
1	312.5
2	289.0
3	759.0
4	1229.0
5	1229.0
6	1747.0
7	2265.0
8	2264.0
9	2263.0
10	3384.5
11	4506.0
12	4506.0
13	7594.0
14	10682.0
15	14509.5
16	18337.0
17	18564.5
18	18792.0
19	18792.0
20	21533.0
21	24274.0
22	24457.0
23	24640.0
24	27316.5
25	29993.0
26	29993.0
27	33627.0
28	37261.0
29	45708.5
30	54156.0
31	58287.5
32	62419.0
33	62419.0
34	67605.5
35	72792.0
36	79993.0
37	87194.0
38	95800.5
39	104407.0
40	104407.0
41	109555.0
42	114703.0
43	113233.0
44	111763.0
45	116315.5
46	120868.0
47	120868.0
48	125489.5
49	130111.0
50	131498.5
51	132886.0
52	139405.5
53	145925.0
54	145925.0
55	136825.0
56	127725.0
57	122557.5
58	117390.0
59	109983.0
60	102576.0
61	102576.0
62	95376.5
63	88177.0
64	78805.0
65	69433.0
66	60925.0
67	52417.0
68	52417.0
69	44608.0
70	36799.0
71	31976.0
72	27153.0
73	21309.0
74	15465.0
75	15465.0
76	12022.0
77	8579.0
78	7014.5
79	5450.0
80	4536.0
81	3622.0
82	3622.0
83	2816.0
84	2010.0
85	1799.5
86	1589.0
87	1436.5
88	1284.0
89	1284.0
90	993.0
91	702.0
92	391.0
93	80.0
94	51.0
95	22.0
96	22.0
97	14.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1972573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.005720993042935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.43807067000195	46.1414460678801
2	8.99375042300762	9.71427952052023
3	2.2275855805377702	3.609070960519452
4	0.908047212522089	1.9615897763191323
5	0.4982940415527708	1.345536449029734
6	0.3141351778506471	1.0179058061461184
7	0.22578704966470595	0.8535654685624107
8	0.1638610835780889	0.7079548769068766
9	0.11935546273068545	0.580129003730604
>10	0.8471885214588163	9.022292235504658
>50	0.12338205474248948	4.704011694259196
>100	0.12848620514283032	14.214560002749742
>500	0.009513345610738872	3.4973331586831002
>1k	0.002260596976809237	1.6557801127986809
>5k	2.825746221011546E-4	0.9745448663900808
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7142	0.36206518085769196	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6266	0.31765617799696133	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5750	0.29149745028447616	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3946	0.20004329370826834	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0139041749025258E-4	0.0
2	0.0	0.0	0.0	0.0015208562623537888	0.0
3	0.0	0.0	0.0	0.002940322107217325	0.0
4	0.0	0.0	0.0	0.005069520874512629	0.0
5	0.0	0.0	0.0	0.005525777753218766	0.0
6	0.0	0.0	0.0	0.005779253796944397	0.0
7	0.0	0.0	0.0	0.006488986719376165	0.0
8	0.0	0.0	0.0	0.007198719641807933	0.0
9	0.0	0.0	0.0	0.012217545307575435	0.0
10	0.0	0.0	0.0	0.017033590138362434	0.0
11	0.0	0.0	0.0	0.01941626494938337	0.0
12	0.0	0.0	0.0	0.020987816420482284	0.0
13	0.0	0.0	0.0	0.02215380622162019	0.0
14	0.0	0.0	0.0	0.023319796022758094	0.0
15	0.0	0.0	0.0	0.02514482353758264	0.0
16	0.0	0.0	0.0	0.027730279183584082	0.0
17	0.0	0.0	0.0	0.03102546775201729	0.0
18	0.0	0.0	0.0	0.03305327610182234	0.0
19	0.0	0.0	0.0	0.034827608407901764	0.0
20	0.0	0.0	0.0	0.03700750238394219	0.0
21	0.0	0.0	0.0	0.040505471787355904	0.0
22	0.0	0.0	0.0	0.045422907035633155	0.0
23	0.0	0.0	0.0	0.05191189375500932	0.0
24	0.0	0.0	0.0	0.06230441154776021	0.0
25	0.0	0.0	0.0	0.06798227492721436	0.0
26	0.0	0.0	0.0	0.07558655623898329	0.0
27	0.0	0.0	0.0	0.09079511886252119	0.0
28	0.0	0.0	0.0	0.12354422371187276	0.0
29	0.0	0.0	0.0	0.17033590138362434	0.0
30	0.0	0.0	0.0	0.23953486132072171	0.0
31	0.0	0.0	0.0	0.4631514270954738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3305	0.0	18.695915	1
AAGACGG	405	0.0	17.358025	5
CAAGACG	480	0.0	15.802083	4
GAGTACG	550	0.0	15.472728	1
TCTAGCG	285	0.0	14.929824	28
CGCAAGA	475	0.0	14.8	2
CTACGGA	75	0.004105793	14.799999	4
GCGCAAG	470	0.0	14.563828	1
CTAGATA	345	0.0	14.47826	3
GTCTAGC	90	8.277721E-4	14.388888	1
CGAATTA	155	1.2113924E-7	14.322581	15
GACGGAC	480	0.0	14.260416	7
TACGACG	325	0.0	14.230769	5
ATCTCGT	315	0.0	14.095239	37
CTAGCGG	315	0.0	14.095239	29
ACTAGCG	355	0.0	14.070422	27
AACGCTA	80	0.0063007297	13.875001	33
GGACCGT	120	3.3027158E-5	13.874999	6
AGACGGA	535	0.0	13.831776	6
ACGGACC	500	0.0	13.69	8
>>END_MODULE