FastQCFastQC Report
Fri 10 Feb 2017
ERR1630801.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630801.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708062
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62480.3657946842679013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57600.3372242927949922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49800.29155850314567033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31160.18242897506062428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG509.094469E-625.8999985
GTGCCGA609.239324E-418.59
ATTCGCG752.068008E-417.26666630
GGTATCA27400.017.0821171
AAGACGG2550.016.6862745
TCTAGCG2500.016.27999928
GTCTAGA1606.2937033E-1016.18751
CGGTCCA2800.015.85714310
AGTCGGT2350.015.74467911
CGCGCTT957.062886E-515.57894812
GGACCGT1201.9367435E-615.4166666
GACGGAC2800.015.1964297
CTAGCGG2800.015.19642929
CGAAATA1101.4526106E-515.13636424
TATAGAC750.004105492514.83
CGGACCA3300.014.5757579
CTTGCGC3200.014.4531263
TATACTG2950.014.4237295
CAAGACG3100.014.3225814
GCCGTCT2750.014.12727436
Produced by FastQC (version 0.11.5)