##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630801.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1708062
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.971939543178173	33.0	31.0	34.0	30.0	34.0
2	32.138310553129806	33.0	31.0	34.0	30.0	34.0
3	32.25410143191524	34.0	31.0	34.0	30.0	34.0
4	35.837013527611994	37.0	35.0	37.0	35.0	37.0
5	35.81476140795826	37.0	35.0	37.0	35.0	37.0
6	35.84367370739469	37.0	35.0	37.0	35.0	37.0
7	35.8135477517795	37.0	35.0	37.0	35.0	37.0
8	35.80848470371684	37.0	35.0	37.0	35.0	37.0
9	37.54155879587509	39.0	37.0	39.0	35.0	39.0
10	37.441352831454594	39.0	37.0	39.0	34.0	39.0
11	37.55233182402044	39.0	37.0	39.0	35.0	39.0
12	37.47658515908673	39.0	37.0	39.0	35.0	39.0
13	37.52928113850668	39.0	37.0	39.0	35.0	39.0
14	38.79027225007055	40.0	38.0	41.0	35.0	41.0
15	38.762427242102454	40.0	38.0	41.0	35.0	41.0
16	38.74552328896726	40.0	38.0	41.0	35.0	41.0
17	38.72868607814002	40.0	38.0	41.0	35.0	41.0
18	38.71851607260158	40.0	38.0	41.0	35.0	41.0
19	38.744953052055486	40.0	38.0	41.0	34.0	41.0
20	38.728368173989	40.0	38.0	41.0	34.0	41.0
21	38.653622643674524	40.0	38.0	41.0	34.0	41.0
22	38.59616454203653	40.0	38.0	41.0	34.0	41.0
23	38.567865218007306	40.0	38.0	41.0	34.0	41.0
24	38.518486448384195	40.0	38.0	41.0	34.0	41.0
25	38.47123816348587	40.0	38.0	41.0	34.0	41.0
26	38.277891551946006	40.0	38.0	41.0	34.0	41.0
27	38.16383772954377	40.0	38.0	41.0	33.0	41.0
28	38.07329593422253	40.0	38.0	41.0	33.0	41.0
29	37.96105820514712	40.0	37.0	41.0	33.0	41.0
30	37.88525533616461	40.0	37.0	41.0	33.0	41.0
31	37.829425395565266	40.0	37.0	41.0	33.0	41.0
32	37.73202670629052	40.0	37.0	41.0	33.0	41.0
33	37.68880052363439	40.0	37.0	41.0	33.0	41.0
34	37.600593538173676	40.0	37.0	41.0	32.0	41.0
35	37.550342434876484	40.0	37.0	41.0	32.0	41.0
36	37.49363723330886	40.0	37.0	41.0	32.0	41.0
37	37.30250365619047	40.0	36.0	41.0	31.0	41.0
38	37.1970742279847	40.0	36.0	41.0	31.0	41.0
39	37.13017911527802	39.0	36.0	41.0	31.0	41.0
40	37.02383988403231	39.0	36.0	41.0	31.0	41.0
41	36.89217019054343	39.0	35.0	41.0	31.0	41.0
42	36.79838085502751	39.0	35.0	41.0	30.0	41.0
43	35.95137939957683	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	5.0
12	2.0
13	3.0
14	2.0
15	3.0
16	5.0
17	20.0
18	34.0
19	123.0
20	235.0
21	521.0
22	912.0
23	1585.0
24	2655.0
25	4204.0
26	6407.0
27	9418.0
28	13689.0
29	19065.0
30	25825.0
31	33695.0
32	43297.0
33	56093.0
34	73884.0
35	98586.0
36	138145.0
37	211249.0
38	373773.0
39	594618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.440284954527414	19.107854398727913	12.125496615462437	25.32636403128224
2	18.992343369268795	20.84432532308546	34.39008654252597	25.773244765119767
3	20.688944546509436	23.048929137232722	29.076052274449054	27.186074041808787
4	14.703915900008313	15.37414918193836	33.96217467515817	35.959760242895165
5	14.794252199276139	36.53175353119501	33.87400457360447	14.799989695924387
6	35.35837692074409	34.035708305670404	15.5690484303263	15.036866343259202
7	29.207253600864608	30.230694201966905	21.272471373989937	19.28958082317855
8	27.531143483081994	32.81725136441183	20.000913315792985	19.65069183671319
9	26.69259078417528	13.614201358030328	20.230647365259575	39.46256049253481
10	17.26898672296439	26.5390249300084	32.68341547320882	23.508572873818398
11	35.57517232981004	21.45337815606225	21.459232744478832	21.512216769648877
12	21.76261751622599	25.353529321535166	28.86247688901222	24.021376273226615
13	30.085734592772397	19.84360052503949	25.105060589135526	24.965604293052593
14	22.978030071507945	21.17592921100054	25.46224902843105	30.38379168906047
15	25.97323750543013	27.405269832125533	22.508082259309088	24.113410403135248
16	25.366526507819977	26.33042594472566	24.186300028921668	24.116747518532698
17	23.576193370029895	26.275978272451468	25.86849891865752	24.27932943886112
18	23.508455782050067	24.959339883446855	27.18619113357712	24.346013200925963
19	23.920033347735618	25.371854183279062	27.090351521197707	23.61776094778761
20	24.59342810741062	24.74892597575498	26.815127319734295	23.842518597100103
21	25.14733071750323	24.942303031154605	26.236576892407886	23.67378935893428
22	24.711515155773036	25.01326064276355	26.426207011220903	23.84901719024251
23	24.084664374009844	25.090833939283236	26.620637892535516	24.203863794171408
24	24.14473245116395	25.49532745298473	26.54095694418587	23.818983151665453
25	24.39911431786434	25.153478035340637	26.37767247324746	24.069735173547567
26	24.01060383054011	25.821252390135722	26.392777311362238	23.775366467961938
27	24.388400421062	25.278239314497952	26.233532506431267	24.099827758008786
28	23.529649392118085	25.517691980736064	26.846156638342165	24.106501988803686
29	23.275150433649365	25.672487298470433	27.10241197333586	23.949950294544344
30	23.422978791168003	26.098935518734095	26.956515629994698	23.521570060103205
31	23.872142814487997	25.899762420802052	26.17346442927716	24.05463033543279
32	23.41636310625727	25.567631619929486	26.57011279450043	24.44589247931281
33	23.242072009095686	25.481744807858263	27.030693265232763	24.245489917813288
34	23.984023999128837	25.22209381158295	26.723854286319813	24.070027902968395
35	23.72864685239763	25.33701938220041	27.290754082697234	23.643579682704726
36	23.202963358472935	25.608145371772217	26.867291702526018	24.321599567228823
37	23.624903545655837	24.959515521099352	27.172257213145656	24.243323720099152
38	22.952094244822494	24.796172504276775	27.88335552222343	24.368377728677295
39	23.254835011843834	24.640440452395758	27.727916199763243	24.376808335997172
40	22.62148563693824	24.836920439656172	28.403711340689036	24.137882582716554
41	22.205165854635254	24.75571729831821	28.712482333779455	24.326634513267084
42	22.561359013899963	24.943356857069592	28.52507695856474	23.97020717046571
43	22.086903168620342	24.409242755825023	28.619101648535008	24.884752427019627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	293.0
1	285.5
2	278.0
3	707.5
4	1137.0
5	1137.0
6	1586.5
7	2036.0
8	1984.0
9	1932.0
10	2729.5
11	3527.0
12	3527.0
13	6004.0
14	8481.0
15	11756.5
16	15032.0
17	14953.0
18	14874.0
19	14874.0
20	17637.0
21	20400.0
22	19989.5
23	19579.0
24	22229.0
25	24879.0
26	24879.0
27	28187.0
28	31495.0
29	36106.5
30	40718.0
31	46121.0
32	51524.0
33	51524.0
34	57743.5
35	63963.0
36	70157.0
37	76351.0
38	81581.0
39	86811.0
40	86811.0
41	90664.0
42	94517.0
43	96808.0
44	99099.0
45	100999.5
46	102900.0
47	102900.0
48	105652.5
49	108405.0
50	110888.0
51	113371.0
52	115728.5
53	118086.0
54	118086.0
55	116286.5
56	114487.0
57	111007.0
58	107527.0
59	101533.0
60	95539.0
61	95539.0
62	88890.5
63	82242.0
64	72963.0
65	63684.0
66	55550.0
67	47416.0
68	47416.0
69	41056.5
70	34697.0
71	29840.5
72	24984.0
73	20080.5
74	15177.0
75	15177.0
76	12059.0
77	8941.0
78	7281.5
79	5622.0
80	4558.0
81	3494.0
82	3494.0
83	2678.5
84	1863.0
85	1561.5
86	1260.0
87	1085.0
88	910.0
89	910.0
90	685.0
91	460.0
92	255.0
93	50.0
94	31.0
95	12.0
96	12.0
97	9.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1708062.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.22380856035966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.24137869231835	54.74525986556574
2	9.457014033128761	12.147309176325924
3	2.3915819200316957	4.60789498165599
4	0.9754566953810689	2.505901762522993
5	0.46007936063349986	1.477402438994928
6	0.3025502372005027	1.1658557108313892
7	0.19368770529639898	0.8707553473815901
8	0.13609802574097515	0.6992586840505031
9	0.10162171198241983	0.5873880038341444
>10	0.5962283272643057	7.48535930292267
>50	0.0773131709032161	3.4916312779017025
>100	0.06333971700504018	7.611148210834343
>500	0.0031028426465698573	1.2871495318423427
>1k	3.650403113611597E-4	0.6138884475441326
>5k	1.8252015568057984E-4	0.7037972577916694
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6248	0.3657946842679013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5760	0.3372242927949922	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4980	0.29155850314567033	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3116	0.18242897506062428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	5.854588416579726E-5	0.0	1.7563765249739178E-4	5.854588416579726E-5
2	0.0	5.854588416579726E-5	0.0	0.0017563765249739179	5.854588416579726E-5
3	0.0	5.854588416579726E-5	0.0	0.002927294208289863	5.854588416579726E-5
4	0.0	5.854588416579726E-5	0.0	0.004625124849097983	5.854588416579726E-5
5	0.0	1.1709176833159452E-4	0.0	0.005620404879916537	5.854588416579726E-5
6	0.0	1.1709176833159452E-4	0.0	0.005620404879916537	5.854588416579726E-5
7	0.0	1.1709176833159452E-4	0.0	0.006322955489906104	5.854588416579726E-5
8	0.0	1.1709176833159452E-4	0.0	0.006615684910735091	5.854588416579726E-5
9	0.0	1.1709176833159452E-4	0.0	0.011474993296496263	5.854588416579726E-5
10	0.0	1.1709176833159452E-4	0.0	0.0170953981764128	5.854588416579726E-5
11	0.0	1.7563765249739178E-4	0.0	0.018559045280557733	5.854588416579726E-5
12	0.0	1.7563765249739178E-4	0.0	0.020022692384702664	5.854588416579726E-5
13	0.0	1.7563765249739178E-4	0.0	0.021427793604681798	5.854588416579726E-5
14	0.0	1.7563765249739178E-4	0.0	0.02289144070882673	5.854588416579726E-5
15	0.0	1.7563765249739178E-4	0.0	0.024179450160474267	5.854588416579726E-5
16	0.0	1.7563765249739178E-4	0.0	0.026989652600432536	5.854588416579726E-5
17	0.0	1.7563765249739178E-4	0.0	0.030209676229551386	5.854588416579726E-5
18	0.0	1.7563765249739178E-4	0.0	0.031556231565364724	5.854588416579726E-5
19	0.0	1.7563765249739178E-4	0.0	0.0341907963528256	5.854588416579726E-5
20	0.0	1.7563765249739178E-4	0.0	0.03694245290861807	5.854588416579726E-5
21	0.0	1.7563765249739178E-4	0.0	0.04098211891605808	5.854588416579726E-5
22	0.0	1.7563765249739178E-4	0.0	0.04712943675346679	5.854588416579726E-5
23	0.0	1.7563765249739178E-4	0.0	0.054623309926688846	5.854588416579726E-5
24	0.0	1.7563765249739178E-4	0.0	0.0662739408756825	5.854588416579726E-5
25	0.0	1.7563765249739178E-4	0.0	0.07130888691394106	5.854588416579726E-5
26	0.0	1.7563765249739178E-4	0.0	0.07938821892882109	5.854588416579726E-5
27	0.0	1.7563765249739178E-4	0.0	0.09642507122106808	5.854588416579726E-5
28	0.0	1.7563765249739178E-4	0.0	0.1344798959288363	5.854588416579726E-5
29	0.0	1.7563765249739178E-4	0.0	0.1882250175930382	5.854588416579726E-5
30	0.0	1.7563765249739178E-4	0.0	0.2649201258502326	5.854588416579726E-5
31	0.0	1.7563765249739178E-4	0.0	0.5178968913306425	5.854588416579726E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	50	9.094469E-6	25.899998	5
GTGCCGA	60	9.239324E-4	18.5	9
ATTCGCG	75	2.068008E-4	17.266666	30
GGTATCA	2740	0.0	17.082117	1
AAGACGG	255	0.0	16.686274	5
TCTAGCG	250	0.0	16.279999	28
GTCTAGA	160	6.2937033E-10	16.1875	1
CGGTCCA	280	0.0	15.857143	10
AGTCGGT	235	0.0	15.744679	11
CGCGCTT	95	7.062886E-5	15.578948	12
GGACCGT	120	1.9367435E-6	15.416666	6
GACGGAC	280	0.0	15.196429	7
CTAGCGG	280	0.0	15.196429	29
CGAAATA	110	1.4526106E-5	15.136364	24
TATAGAC	75	0.0041054925	14.8	3
CGGACCA	330	0.0	14.575757	9
CTTGCGC	320	0.0	14.453126	3
TATACTG	295	0.0	14.423729	5
CAAGACG	310	0.0	14.322581	4
GCCGTCT	275	0.0	14.127274	36
>>END_MODULE