FastQCFastQC Report
Fri 10 Feb 2017
ERR1630800.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630800.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189546
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65410.29873772919134833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55390.25297481761059143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51870.23689842551834947No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC41810.19095282766381708No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39540.18058538162705876No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT26680.12185174460824298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.017.9447081
TTAACGG1801.0913936E-1116.44444535
CCAGGAC17250.016.0869563
GTATTAG6250.015.9841
TGCATCA14200.015.373239514
AACGCTA855.3669047E-415.23529433
ACGGACC4150.015.1566278
GCATCAG14300.015.13636415
ACGCTGA6400.015.0312511
ACACGCT6050.014.9834719
CGCAAGA4350.014.8850582
GGACCGT750.00410598414.86
TAACGGC2006.184564E-1114.79999936
CGGACCA4200.014.5357149
CGCTAAG908.2782336E-414.38888835
ACTACGC908.2782336E-414.3888888
GACAGGC14950.014.3545147
CACACGC6550.014.1221388
GCTGCAT15250.013.9508212
CTGCATC15800.013.93354413
Produced by FastQC (version 0.11.5)