##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630800.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2189546
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.958208688011123	33.0	31.0	34.0	30.0	34.0
2	32.12667648909866	33.0	31.0	34.0	30.0	34.0
3	32.22790386682902	34.0	31.0	34.0	30.0	34.0
4	35.81908669651151	37.0	35.0	37.0	35.0	37.0
5	35.7967464488072	37.0	35.0	37.0	35.0	37.0
6	35.82174660865768	37.0	35.0	37.0	35.0	37.0
7	35.797410056696684	37.0	35.0	37.0	35.0	37.0
8	35.78635388340779	37.0	35.0	37.0	35.0	37.0
9	37.511307823631014	39.0	37.0	39.0	35.0	39.0
10	37.409689953990465	39.0	37.0	39.0	34.0	39.0
11	37.51593937738691	39.0	37.0	39.0	35.0	39.0
12	37.444625963555914	39.0	37.0	39.0	35.0	39.0
13	37.49222806919791	39.0	37.0	39.0	35.0	39.0
14	38.76058507106039	40.0	38.0	41.0	35.0	41.0
15	38.72743938697794	40.0	38.0	41.0	35.0	41.0
16	38.71576573408369	40.0	38.0	41.0	35.0	41.0
17	38.692984755743886	40.0	38.0	41.0	35.0	41.0
18	38.659142123526976	40.0	38.0	41.0	34.0	41.0
19	38.68604130719336	40.0	38.0	41.0	34.0	41.0
20	38.6622761065536	40.0	38.0	41.0	34.0	41.0
21	38.59545677505748	40.0	38.0	41.0	34.0	41.0
22	38.533732563736955	40.0	38.0	41.0	34.0	41.0
23	38.50803910947749	40.0	38.0	41.0	34.0	41.0
24	38.45914952232106	40.0	38.0	41.0	34.0	41.0
25	38.407279408608	40.0	38.0	41.0	34.0	41.0
26	38.20578284265323	40.0	38.0	41.0	33.0	41.0
27	38.096709089464206	40.0	38.0	41.0	33.0	41.0
28	37.99266194909812	40.0	37.0	41.0	33.0	41.0
29	37.88623075285927	40.0	37.0	41.0	33.0	41.0
30	37.813481881632086	40.0	37.0	41.0	33.0	41.0
31	37.774397523504874	40.0	37.0	41.0	33.0	41.0
32	37.68103159285076	40.0	37.0	41.0	33.0	41.0
33	37.64036882531813	40.0	37.0	41.0	33.0	41.0
34	37.551553153028074	40.0	37.0	41.0	32.0	41.0
35	37.493325556987614	40.0	37.0	41.0	32.0	41.0
36	37.44961375554567	40.0	37.0	41.0	32.0	41.0
37	37.25876049190106	40.0	36.0	41.0	31.0	41.0
38	37.14762147038701	40.0	36.0	41.0	31.0	41.0
39	37.08939113405245	39.0	36.0	41.0	31.0	41.0
40	36.97905410528027	39.0	36.0	41.0	31.0	41.0
41	36.840554617258555	39.0	35.0	41.0	30.0	41.0
42	36.75140508580318	39.0	35.0	41.0	30.0	41.0
43	35.872200903749	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	2.0
12	2.0
13	2.0
14	4.0
15	10.0
16	9.0
17	35.0
18	85.0
19	157.0
20	348.0
21	676.0
22	1116.0
23	2202.0
24	3524.0
25	5678.0
26	8592.0
27	12494.0
28	17989.0
29	24955.0
30	33507.0
31	43831.0
32	56377.0
33	73191.0
34	96848.0
35	129872.0
36	178598.0
37	272356.0
38	492642.0
39	734436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72883693697232	18.708535924799023	11.715579394084436	25.84704774414422
2	19.373513961341757	20.34969806526102	34.27646644555538	26.000321527841848
3	21.080945547615805	23.15247087752438	28.162230891700833	27.60435268315897
4	14.939809439947824	14.959037170262693	33.45282538023864	36.64832800955084
5	14.958032395756929	36.71989535730238	33.40962007649074	14.912452170449948
6	36.207871403478165	33.434876453840204	15.207855875144894	15.14939626753674
7	29.889164237700417	29.42610020524803	21.327023958391376	19.35771159866018
8	27.78534910890203	32.78771032899058	19.6391398034113	19.787800758696093
9	27.315023297067064	13.509512930991175	19.548984127303104	39.626479644638664
10	18.101606451748445	25.906877498805684	32.00142860666092	23.990087442784944
11	36.2370555357138	21.44060914911128	21.024267131176966	21.298068183997962
12	22.8018502465808	24.797606444440994	28.16780282305099	24.23274048592722
13	30.003982560768307	19.58899242125993	25.036879791518423	25.37014522645334
14	23.201248112622434	20.37888219749665	25.048252012060946	31.37161767781997
15	26.225208330859456	26.844286441116104	22.392724336460617	24.53778089156382
16	25.813616156043306	25.423306932122003	23.64654590495016	25.116531006884536
17	24.20515485858712	26.02662835126551	25.13904709012736	24.629169700020004
18	24.01671396718772	24.486354705496023	26.5470558736834	24.949875453632853
19	24.75718710636817	24.664565165564003	26.167981855599287	24.41026587246854
20	24.66136815577293	24.48713112215957	26.421961447715645	24.429539274351853
21	25.952412052544226	24.516223911258315	25.591332632426994	23.940031403770462
22	25.508393064132928	24.588339317831185	25.68098592128231	24.222281696753573
23	24.687446621354383	24.855700679501595	25.85718683233876	24.599665866805264
24	24.878719149997305	25.21472487903885	25.420657981152257	24.485897989811587
25	24.897444493059293	24.663514719489793	25.883310969488655	24.555729817962263
26	24.89210091955136	25.340961094217707	25.97154843972221	23.795389546508726
27	25.463817613331713	24.648945489156198	25.330593648180947	24.556643249331138
28	24.284714730816344	24.574866205140246	26.565644202040055	24.574774862003355
29	24.22433691733355	24.903473140093883	26.624697540037978	24.24749240253459
30	23.846084987481422	25.251307805362387	26.486906418042828	24.41570078911336
31	24.252287917221196	25.253819741626803	25.827637327555575	24.666255013596423
32	23.827999046377652	24.960242899669613	26.136011757688582	25.075746296264157
33	24.197847407636104	24.988285242694147	26.10175808135568	24.71210926831407
34	24.792856601322832	24.932200556645075	26.122447301860753	24.152495540171344
35	24.659038905782293	24.886940032317202	26.459412133839617	23.994608928060885
36	24.231461682010792	25.339088559911506	25.48149251031949	24.94795724775821
37	24.032607673006183	24.50626750933755	26.452515727004595	25.008609090651667
38	24.13728690787953	24.28160906416216	26.79893457365134	24.782169454306967
39	23.66084110587309	24.51439704852056	26.83953659799794	24.98522524760841
40	23.73606217910014	24.219358716373165	27.41801268390799	24.626566420618705
41	22.88415041291665	24.331117044355313	27.8723534467876	24.912379095940437
42	23.210565112584984	24.705669577163487	27.467474992532697	24.616290317718832
43	23.14456969618359	23.443672797922492	27.875413441873338	25.53634406402058
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	702.0
1	545.0
2	388.0
3	912.5
4	1437.0
5	1437.0
6	1890.5
7	2344.0
8	2287.0
9	2230.0
10	3199.0
11	4168.0
12	4168.0
13	7263.5
14	10359.0
15	13152.0
16	15945.0
17	15884.5
18	15824.0
19	15824.0
20	18113.0
21	20402.0
22	19508.5
23	18615.0
24	20933.5
25	23252.0
26	23252.0
27	27750.0
28	32248.0
29	37652.0
30	43056.0
31	49683.5
32	56311.0
33	56311.0
34	65911.0
35	75511.0
36	84924.0
37	94337.0
38	103880.0
39	113423.0
40	113423.0
41	119166.0
42	124909.0
43	129943.5
44	134978.0
45	136039.0
46	137100.0
47	137100.0
48	140283.0
49	143466.0
50	145110.0
51	146754.0
52	149696.5
53	152639.0
54	152639.0
55	150552.5
56	148466.0
57	141792.0
58	135118.0
59	127663.0
60	120208.0
61	120208.0
62	113980.0
63	107752.0
64	95878.5
65	84005.0
66	73248.5
67	62492.0
68	62492.0
69	54157.0
70	45822.0
71	40801.5
72	35781.0
73	33276.5
74	30772.0
75	30772.0
76	27282.0
77	23792.0
78	18895.0
79	13998.0
80	9635.5
81	5273.0
82	5273.0
83	3800.5
84	2328.0
85	1922.0
86	1516.0
87	1314.0
88	1112.0
89	1112.0
90	849.0
91	586.0
92	342.0
93	98.0
94	60.5
95	23.0
96	23.0
97	13.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2189546.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.24737207383314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.04254088862209	44.75044444726796
2	9.173045654775558	9.768811500601851
3	2.648362991227274	4.230551087413452
4	1.167014375607831	2.48561794694009
5	0.6461177253939253	1.7202035463774548
6	0.40419688384951613	1.2913453119251463
7	0.28236800588451605	1.0524747989755394
8	0.19979031866908215	0.8510647547937847
9	0.16085684727752686	0.7708683957849205
>10	1.034516198887069	10.76629536214819
>50	0.1255136587681571	4.645582905312963
>100	0.10302674483182728	11.351187568174419
>500	0.009982081087548488	3.583862448158942
>1k	0.0024094678487185995	1.9405046944899462
>5k	2.5815726950556426E-4	0.7911852316353696
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6541	0.29873772919134833	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5539	0.25297481761059143	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5187	0.23689842551834947	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4181	0.19095282766381708	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3954	0.18058538162705876	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2668	0.12185174460824298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.134313688773838E-5	0.0
2	1.8268627377547675E-4	0.0	0.0	0.0011874607795405987	0.0
3	2.740294106632151E-4	0.0	0.0	0.0021465637168618518	0.0
4	2.740294106632151E-4	0.0	0.0	0.0038364117492850117	0.0
5	6.850735266580378E-4	0.0	0.0	0.004110441159948227	0.0
6	6.850735266580378E-4	0.0	0.0	0.004247455865279835	0.0
7	6.850735266580378E-4	0.0	0.0	0.004795514686606265	0.0
8	6.850735266580378E-4	0.0	0.0	0.005160887234157218	0.0
9	6.850735266580378E-4	0.0	0.0	0.008083867614564847	0.0
10	7.30745095101907E-4	0.0	0.0	0.011737593090074381	0.0
11	8.677598004335146E-4	0.0	0.0	0.013564455827829149	0.0
12	9.591029373212529E-4	0.0	0.0	0.014934602881145224	0.0
13	9.591029373212529E-4	0.0	0.0	0.015711019544691	0.0
14	9.591029373212529E-4	0.0	0.0	0.016852808755787728	0.0
15	9.591029373212529E-4	0.0	0.0	0.01854265678821089	0.0
16	9.591029373212529E-4	0.0	0.0	0.02073489207351661	4.567156844386919E-5
17	0.0010504460742089912	0.0	0.0	0.022881455790378463	4.567156844386919E-5
18	0.0011874607795405987	0.0	0.0	0.024753990096577097	4.567156844386919E-5
19	0.0013701470533160757	0.0	0.0	0.02699189695032669	4.567156844386919E-5
20	0.0013701470533160757	0.0	0.0	0.030006220467622056	9.134313688773838E-5
21	0.0014158186217599449	0.0	0.0	0.0337969606484632	9.134313688773838E-5
22	0.0015071617586476832	0.0	0.0	0.039734264546166194	9.134313688773838E-5
23	0.0015528333270915524	0.0	0.0	0.04854887725583294	9.134313688773838E-5
24	0.0015528333270915524	0.0	0.0	0.06124557328322858	9.134313688773838E-5
25	0.00168984803242316	0.0	0.0	0.06626944581205418	9.134313688773838E-5
26	0.00168984803242316	0.0	0.0	0.07439898499506291	9.134313688773838E-5
27	0.00168984803242316	0.0	0.0	0.09362671530993183	9.134313688773838E-5
28	0.0017811911693108983	0.0	0.0	0.1330869504454348	9.134313688773838E-5
29	0.0017811911693108983	0.0	0.0	0.18953700904205711	9.134313688773838E-5
30	0.0018725343061986367	0.0	0.0	0.2704670283245933	9.134313688773838E-5
31	0.0018725343061986367	0.0	0.0	0.50343769895677	9.134313688773838E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3165	0.0	17.944708	1
TTAACGG	180	1.0913936E-11	16.444445	35
CCAGGAC	1725	0.0	16.086956	3
GTATTAG	625	0.0	15.984	1
TGCATCA	1420	0.0	15.3732395	14
AACGCTA	85	5.3669047E-4	15.235294	33
ACGGACC	415	0.0	15.156627	8
GCATCAG	1430	0.0	15.136364	15
ACGCTGA	640	0.0	15.03125	11
ACACGCT	605	0.0	14.983471	9
CGCAAGA	435	0.0	14.885058	2
GGACCGT	75	0.004105984	14.8	6
TAACGGC	200	6.184564E-11	14.799999	36
CGGACCA	420	0.0	14.535714	9
CGCTAAG	90	8.2782336E-4	14.388888	35
ACTACGC	90	8.2782336E-4	14.388888	8
GACAGGC	1495	0.0	14.354514	7
CACACGC	655	0.0	14.122138	8
GCTGCAT	1525	0.0	13.95082	12
CTGCATC	1580	0.0	13.933544	13
>>END_MODULE