FastQCFastQC Report
Fri 10 Feb 2017
ERR1630799.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630799.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815654
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46500.2561060642611423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.22113244043193253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35090.1932636945144835No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC24990.1376363558255042No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT22810.12562966292035815No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA22180.12215983882391689No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22160.12204968567799811No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA21660.11929585703002886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.021.3270781
AAGACGG4150.019.6144585
CGCAAGA4150.018.722892
ACGGACC4100.018.58
GACGGAC4150.018.277117
CAAGACG4650.017.9032254
CGAATTA1451.546141E-1017.86206815
CGAACGA2200.017.65909216
GCTTTAT8100.016.9012341
CGGACCA4650.016.7096779
ATAACGA2450.016.61224412
GCGCAAG4950.016.0707051
TAACGAA2450.015.85714213
ACGAACG2450.015.85714215
GCGAAAG4900.015.47959118
GAGCGAA5150.015.44660116
CGCAATA2900.015.31034536
AGCGAAA5050.015.01980117
CGAAAGC4950.014.94949319
GCGTTAT2600.014.9423071
Produced by FastQC (version 0.11.5)