##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630799.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1815654
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.996660156615743	33.0	31.0	34.0	30.0	34.0
2	32.167981895228934	33.0	31.0	34.0	30.0	34.0
3	32.2834482781411	34.0	31.0	34.0	30.0	34.0
4	35.862741469465	37.0	35.0	37.0	35.0	37.0
5	35.83761608764666	37.0	35.0	37.0	35.0	37.0
6	35.86677692996573	37.0	35.0	37.0	35.0	37.0
7	35.84405729285425	37.0	35.0	37.0	35.0	37.0
8	35.83441118186615	37.0	35.0	37.0	35.0	37.0
9	37.56094332951102	39.0	37.0	39.0	35.0	39.0
10	37.47176995176394	39.0	37.0	39.0	35.0	39.0
11	37.572166833548685	39.0	37.0	39.0	35.0	39.0
12	37.5009082126881	39.0	37.0	39.0	35.0	39.0
13	37.54939707675582	39.0	37.0	39.0	35.0	39.0
14	38.828409487710765	40.0	38.0	41.0	35.0	41.0
15	38.80805979553373	40.0	38.0	41.0	35.0	41.0
16	38.78870919239018	40.0	38.0	41.0	35.0	41.0
17	38.77807831227756	40.0	38.0	41.0	35.0	41.0
18	38.763898297803436	40.0	38.0	41.0	35.0	41.0
19	38.77416126640869	40.0	38.0	41.0	35.0	41.0
20	38.76538977139918	40.0	38.0	41.0	35.0	41.0
21	38.69345866558276	40.0	38.0	41.0	34.0	41.0
22	38.637796628652815	40.0	38.0	41.0	34.0	41.0
23	38.628263975405005	40.0	38.0	41.0	34.0	41.0
24	38.57422063895434	40.0	38.0	41.0	34.0	41.0
25	38.52560785259747	40.0	38.0	41.0	34.0	41.0
26	38.35269935791731	40.0	38.0	41.0	34.0	41.0
27	38.24322090001729	40.0	38.0	41.0	34.0	41.0
28	38.14639683552043	40.0	38.0	41.0	33.0	41.0
29	38.0588008508229	40.0	38.0	41.0	33.0	41.0
30	37.993498210562144	40.0	37.0	41.0	33.0	41.0
31	37.95731180059637	40.0	37.0	41.0	33.0	41.0
32	37.88103350087627	40.0	37.0	41.0	33.0	41.0
33	37.837762040564996	40.0	37.0	41.0	33.0	41.0
34	37.776399027568026	40.0	37.0	41.0	33.0	41.0
35	37.72174764575189	40.0	37.0	41.0	33.0	41.0
36	37.691578902147654	40.0	37.0	41.0	33.0	41.0
37	37.51714368486507	40.0	37.0	41.0	32.0	41.0
38	37.41645710030656	40.0	36.0	41.0	32.0	41.0
39	37.361464794503796	40.0	36.0	41.0	32.0	41.0
40	37.261813649516924	40.0	36.0	41.0	31.0	41.0
41	37.158285664559436	39.0	36.0	41.0	31.0	41.0
42	37.08131670461442	39.0	36.0	41.0	31.0	41.0
43	36.236279048761496	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	5.0
11	4.0
12	4.0
13	1.0
14	7.0
15	1.0
16	12.0
17	26.0
18	52.0
19	87.0
20	213.0
21	451.0
22	837.0
23	1434.0
24	2484.0
25	3871.0
26	6104.0
27	8934.0
28	12985.0
29	18547.0
30	25001.0
31	33617.0
32	43856.0
33	57088.0
34	75903.0
35	103030.0
36	144878.0
37	221521.0
38	408898.0
39	645796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86760913698315	18.91505760458766	11.885359214916498	25.331974043512695
2	19.47309344181215	20.42134679845389	33.25121416305089	26.854345596683064
3	21.063649792306244	22.464081812944535	28.689111471679073	27.783156923070145
4	14.83542569234006	14.78789460987611	34.02724307604863	36.3494366217352
5	15.022080198099419	36.145708378358435	33.985329804026534	14.846881619515612
6	36.14416623431557	33.85198942089186	14.998837884310557	15.00500646048201
7	29.86312370088133	29.775662103021833	20.90161451465973	19.4595996814371
8	28.46819933753898	31.50737971001083	19.800688897774577	20.223732054675615
9	26.773217804713894	13.691154812535869	19.813081126690438	39.7225462560598
10	17.89878468034108	25.933134837364385	32.390422404268655	23.77765807802588
11	36.38584223646135	21.34900151680882	20.90161451465973	21.363541732070097
12	22.980094225001018	24.97149787349352	27.817029015440163	24.231378886065297
13	30.852188798085976	19.25284222654757	24.60749680280494	25.287472172561515
14	23.33484243143242	20.672495971148688	24.458129136939085	31.534532460479802
15	26.303965403099934	27.428794252649457	21.75921183221032	24.50802851204029
16	25.94569229599913	25.817308804430798	23.556801020458742	24.68019787911133
17	24.653595894371946	25.57337466279368	24.678600658495505	25.094428784338866
18	24.1988286314463	24.283316094365997	26.56029177365291	24.957563500534793
19	25.18271653079276	24.589156304009467	26.257535852095167	23.970591313102606
20	25.82496444807216	24.093246841083158	25.32343717470399	24.758351536140697
21	25.840110505635987	24.31961155594623	25.508990149004163	24.331287789413622
22	25.740532061725418	24.39446061859804	25.66579315221953	24.199214167457015
23	24.982678417804273	24.245423412169938	25.536638588629774	25.235259581396015
24	24.66835641592506	24.903313076169802	25.893865240844345	24.534465267060796
25	25.15369117684316	24.80891183011741	25.241923846724102	24.795473146315324
26	24.86718284430844	25.41299168233595	25.44559701352791	24.2742284598277
27	25.384682323834827	24.095560057147452	25.45193081941824	25.067826799599484
28	24.495195670540753	24.810288744441397	25.74537880014584	24.94913678487201
29	24.422384441088447	25.203921011382125	26.039267393457123	24.33442715407231
30	24.57973821003341	25.26356893989714	25.54187086306091	24.614821987008536
31	24.248287393963828	25.721145108043714	25.57750540576564	24.453062092226823
32	24.17834014630541	24.731584321682433	25.47208884512137	25.617986686890788
33	23.605929323538515	25.21780030776789	26.07820653053941	25.098063838154182
34	25.40869570964512	24.186436402530436	25.607081525444826	24.797786362379618
35	24.44402953426148	24.333766235196794	26.3082062992178	24.91399793132392
36	23.698513042683242	25.377852828787862	26.11758628020537	24.806047848323523
37	24.650842065723978	24.12375926250266	26.49579710671747	24.729601565055898
38	24.311405146575282	24.4139026488527	26.353148782752662	24.921543421819354
39	24.26960202769911	24.135380419397087	26.515734826128767	25.079282726775038
40	23.53201656262702	24.061247352193753	27.183373043542435	25.223363041636787
41	22.66703898429987	24.382013313109216	27.703406045424956	25.247541657165957
42	23.576022744421568	24.128826307214922	27.821875753860592	24.473275194502918
43	23.54198542233267	23.70126687133121	27.50039379749666	25.256353908839458
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	199.0
1	203.0
2	207.0
3	530.5
4	854.0
5	854.0
6	1122.5
7	1391.0
8	1274.5
9	1158.0
10	1733.0
11	2308.0
12	2308.0
13	4156.5
14	6005.0
15	8293.0
16	10581.0
17	10788.5
18	10996.0
19	10996.0
20	13825.5
21	16655.0
22	16985.5
23	17316.0
24	20449.5
25	23583.0
26	23583.0
27	26840.0
28	30097.0
29	34525.0
30	38953.0
31	44908.0
32	50863.0
33	50863.0
34	57171.0
35	63479.0
36	69515.0
37	75551.0
38	81620.5
39	87690.0
40	87690.0
41	91257.0
42	94824.0
43	96471.0
44	98118.0
45	101460.0
46	104802.0
47	104802.0
48	110859.0
49	116916.0
50	117918.0
51	118920.0
52	122281.0
53	125642.0
54	125642.0
55	123087.0
56	120532.0
57	118148.0
58	115764.0
59	115640.0
60	115516.0
61	115516.0
62	107959.5
63	100403.0
64	92848.5
65	85294.0
66	74421.0
67	63548.0
68	63548.0
69	55719.5
70	47891.0
71	40558.5
72	33226.0
73	24948.0
74	16670.0
75	16670.0
76	12178.5
77	7687.0
78	6163.0
79	4639.0
80	3899.5
81	3160.0
82	3160.0
83	2393.0
84	1626.0
85	1385.0
86	1144.0
87	1005.5
88	867.0
89	867.0
90	681.5
91	496.0
92	276.5
93	57.0
94	36.5
95	16.0
96	16.0
97	12.5
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1815654.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.02324188454623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.07055971358218	50.52116147284005
2	8.269127586665036	9.596031802704786
3	1.9345881906213829	3.3675323559423336
4	0.7491763293424233	1.7387855748644758
5	0.4276340399986105	1.2406356670452545
6	0.24801855301589765	0.8634504296097948
7	0.17333622707783175	0.7040270881764163
8	0.1377632656890121	0.6394777030302845
9	0.10277027684532104	0.5366758168744059
>10	0.6765540269981324	7.831484136942728
>50	0.09754173087938309	3.969389288606014
>100	0.09957732079423327	11.972991407937526
>500	0.010396060681984795	4.110759221976644
>1k	0.002956677808637877	2.9075980334493727
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4650	0.2561060642611423	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4015	0.22113244043193253	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3509	0.1932636945144835	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2499	0.1376363558255042	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2281	0.12562966292035815	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2218	0.12215983882391689	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2216	0.12204968567799811	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2166	0.11929585703002886	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.5076572959385434E-5	0.0
2	0.0	0.0	0.0	6.058423025532397E-4	0.0
3	0.0	0.0	0.0	0.0011566080321470942	0.0
4	0.0	0.0	0.0	0.0018726034806191047	0.0
5	0.0	0.0	0.0	0.002037833199497261	0.0
6	0.0	0.0	0.0	0.002037833199497261	0.0
7	0.0	0.0	0.0	0.0022030629183754174	0.0
8	0.0	0.0	0.0	0.002368292637253574	0.0
9	0.0	0.0	0.0	0.0038553601071569803	0.0
10	0.0	0.0	0.0	0.005177197858182231	0.0
11	0.0	0.0	0.0	0.005893193306654242	0.0
12	0.0	0.0	0.0	0.006443959036248096	0.0
13	0.0	0.0	0.0	0.006774418474004409	0.0
14	0.0	0.0	0.0	0.007049801338801336	0.0
15	0.0	0.0	0.0	0.008041179652070274	0.0
16	0.0	0.0	0.0	0.009473170549014295	0.0
17	0.0	0.0	0.0	0.010684855154120773	0.0
18	0.0	0.0	0.0	0.011125467737795857	0.0
19	0.0	0.0	0.0	0.012722688353618035	0.0
20	0.0	0.0	0.0	0.0139894495316839	0.0
21	0.0	0.0	0.0	0.01591712958526239	0.0
22	0.0	0.0	0.0	0.01955218340058183	0.0
23	0.0	0.0	0.0	0.02318723721590127	0.0
24	0.0	0.0	0.0	0.028860124230717966	0.0
25	0.0	0.0	0.0	0.031008110576134	0.0
26	0.0	0.0	0.0	0.03585484899655992	0.0
27	0.0	0.0	0.0	0.04642955100476192	0.0
28	0.0	0.0	0.0	0.07468383293292664	0.0
29	0.0	0.0	0.0	0.12067277135401348	0.0
30	0.0	0.0	0.0	0.17800748380473372	0.0
31	0.0	0.0	0.0	0.36345030495898445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1865	0.0	21.327078	1
AAGACGG	415	0.0	19.614458	5
CGCAAGA	415	0.0	18.72289	2
ACGGACC	410	0.0	18.5	8
GACGGAC	415	0.0	18.27711	7
CAAGACG	465	0.0	17.903225	4
CGAATTA	145	1.546141E-10	17.862068	15
CGAACGA	220	0.0	17.659092	16
GCTTTAT	810	0.0	16.901234	1
CGGACCA	465	0.0	16.709677	9
ATAACGA	245	0.0	16.612244	12
GCGCAAG	495	0.0	16.070705	1
TAACGAA	245	0.0	15.857142	13
ACGAACG	245	0.0	15.857142	15
GCGAAAG	490	0.0	15.479591	18
GAGCGAA	515	0.0	15.446601	16
CGCAATA	290	0.0	15.310345	36
AGCGAAA	505	0.0	15.019801	17
CGAAAGC	495	0.0	14.949493	19
GCGTTAT	260	0.0	14.942307	1
>>END_MODULE