FastQCFastQC Report
Fri 10 Feb 2017
ERR1630798.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630798.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708109
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59550.3486311470755087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50320.2945947828856355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43770.25624828392099097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.17305687166334233No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG17160.10046197286004582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC551.9033609E-523.5454543
TAGTACT1355.638867E-1119.1851864
CCTAGTA1201.04204446E-716.9583342
AAGACGG2650.016.7547175
GCGCAAG3150.016.4444451
ACGGACC2850.016.228078
CTAGTAG803.3837702E-416.18753
CAAGACG2750.016.1454544
GGTATCA29300.016.1006831
TACGGGT700.002593185815.8571424
CGCAAGA3350.015.4626872
TCTAGCG1954.1836756E-1115.17948628
GACGGAC2950.015.0508477
CGGACCA3050.014.5573769
GTCGGTT1304.4467197E-614.23076912
AGAGCGA3150.014.09523915
AGCGTCA1051.6563751E-414.0952383
GTCCTAT2252.7284841E-1113.97777751
GCGAAAG3050.013.95081918
GAGCGAA3200.013.87516
Produced by FastQC (version 0.11.5)