##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630798.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1708109
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98336991374672	33.0	31.0	34.0	30.0	34.0
2	32.15229882870472	33.0	31.0	34.0	30.0	34.0
3	32.25470856953508	34.0	31.0	34.0	30.0	34.0
4	35.84036850107341	37.0	35.0	37.0	35.0	37.0
5	35.820020853470126	37.0	35.0	37.0	35.0	37.0
6	35.85074430261769	37.0	35.0	37.0	35.0	37.0
7	35.82356219655772	37.0	35.0	37.0	35.0	37.0
8	35.81585191577353	37.0	35.0	37.0	35.0	37.0
9	37.545104557144775	39.0	37.0	39.0	35.0	39.0
10	37.44753642771041	39.0	37.0	39.0	34.0	39.0
11	37.55392835000577	39.0	37.0	39.0	35.0	39.0
12	37.48285384597821	39.0	37.0	39.0	35.0	39.0
13	37.53383946809015	39.0	37.0	39.0	35.0	39.0
14	38.806880591344	40.0	38.0	41.0	35.0	41.0
15	38.78160995580493	40.0	38.0	41.0	35.0	41.0
16	38.761385251175426	40.0	38.0	41.0	35.0	41.0
17	38.74783986267855	40.0	38.0	41.0	35.0	41.0
18	38.73642080218534	40.0	38.0	41.0	35.0	41.0
19	38.766442305496895	40.0	38.0	41.0	35.0	41.0
20	38.74901484624225	40.0	38.0	41.0	34.0	41.0
21	38.6764462923619	40.0	38.0	41.0	34.0	41.0
22	38.627089371931184	40.0	38.0	41.0	34.0	41.0
23	38.602951568079085	40.0	38.0	41.0	34.0	41.0
24	38.55900882203653	40.0	38.0	41.0	34.0	41.0
25	38.517693542976474	40.0	38.0	41.0	34.0	41.0
26	38.332353497347064	40.0	38.0	41.0	34.0	41.0
27	38.22157426721597	40.0	38.0	41.0	34.0	41.0
28	38.13523785660048	40.0	38.0	41.0	33.0	41.0
29	38.03860292288139	40.0	37.0	41.0	33.0	41.0
30	37.96637743844216	40.0	37.0	41.0	33.0	41.0
31	37.91362202295053	40.0	37.0	41.0	33.0	41.0
32	37.81795833872429	40.0	37.0	41.0	33.0	41.0
33	37.78887530011258	40.0	37.0	41.0	33.0	41.0
34	37.7097960376065	40.0	37.0	41.0	33.0	41.0
35	37.66227974912608	40.0	37.0	41.0	33.0	41.0
36	37.61832939232801	40.0	37.0	41.0	33.0	41.0
37	37.44114632028752	40.0	37.0	41.0	32.0	41.0
38	37.329644653824786	40.0	36.0	41.0	31.0	41.0
39	37.27071047573662	40.0	36.0	41.0	31.0	41.0
40	37.179048878028276	40.0	36.0	41.0	31.0	41.0
41	37.0561784991473	40.0	36.0	41.0	31.0	41.0
42	36.97237178657802	39.0	36.0	41.0	31.0	41.0
43	36.13007132448807	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	2.0
11	2.0
12	5.0
13	4.0
14	2.0
15	1.0
16	6.0
17	21.0
18	53.0
19	111.0
20	237.0
21	467.0
22	780.0
23	1464.0
24	2416.0
25	3937.0
26	6004.0
27	8913.0
28	13116.0
29	18023.0
30	24568.0
31	32643.0
32	42468.0
33	55087.0
34	72611.0
35	96840.0
36	135898.0
37	207794.0
38	376602.0
39	608028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.135361970459726	19.418784164242446	12.283349598883913	25.16250426641391
2	18.442441319611337	21.381773645592876	35.030609873257504	25.145175161538287
3	20.302627057172582	23.67144017155814	29.748452821219256	26.277479950050026
4	14.644381593914673	15.61516273258908	34.485328512407584	35.255127161088666
5	14.26770773996273	37.24850112024467	34.21666884256215	14.267122297230447
6	34.56816865902586	34.627708184899205	15.917134093901502	14.886989062173434
7	28.494785754304907	30.827775042459233	21.744221241150303	18.933217962085557
8	26.82551289174169	33.82828613396452	20.346242540727786	18.999958433566007
9	26.543680760419857	14.23650364233196	20.206790081897584	39.013025515350606
10	16.94956235228548	27.088025412898126	33.11123587546228	22.851176359354117
11	34.97071908174478	22.158187797148777	21.809556650073265	21.061536471033172
12	21.20461867480354	25.985519659459673	29.321313803744374	23.488547861992416
13	29.455204556617876	20.234891333047248	25.92949278998003	24.38041132035485
14	22.670157466531702	21.41848090490712	25.492986688788594	30.41837493977258
15	25.28890135231417	27.988026525239313	23.06228700861596	23.660785113830556
16	24.889629408896035	26.789566707979407	24.48871822582751	23.832085657297046
17	23.333698259303123	26.910226455103274	25.931132029630426	23.824943255963174
18	22.968967437089788	25.426831660040435	27.559775166573093	24.044425736296688
19	23.819674271372612	25.963682645545454	27.467216670598894	22.749426412483047
20	24.229952538157693	25.152903005604443	27.450297375635863	23.166847080602
21	24.16760288716938	25.849052958564116	26.54584689852931	23.437497255737192
22	24.229952538157693	25.471793661879893	26.67195126306342	23.62630253689899
23	23.463022558864804	25.926858297684753	26.908353038359966	23.70176610509048
24	24.145648784708705	26.10805282332685	26.640512988339736	23.105785403624708
25	24.07486875837549	25.54473982632256	26.78833727824161	23.59205413706034
26	23.83454451677264	26.115312313207177	26.629799386338927	23.420343783681254
27	24.1160253824551	25.649182809762138	26.354524213618685	23.880267594164074
28	23.278081199736082	25.895127301594922	27.166357650477806	23.660433848191186
29	23.165266385224832	26.361725159225784	27.057699479365777	23.415308976183606
30	22.97493895295909	26.898341967637897	26.97603021821207	23.150688861190943
31	23.641524047938393	26.309386578959536	26.675229742364216	23.37385963073785
32	22.97769053380083	26.226136622428665	26.86895274247721	23.9272201012933
33	23.015217412940274	25.966726947753333	27.14610133194076	23.87195430736563
34	23.50365228448536	25.9286146258816	26.88253501386621	23.685198075766827
35	23.69345281829204	25.98669054492424	27.05459663288467	23.265260003899048
36	23.08248478287978	26.364769461433667	26.908353038359966	23.644392717326586
37	23.803633140508012	25.34674309426389	26.993769133000296	23.855854632227803
38	23.097589205372728	25.647777747204657	27.636468164502382	23.618164882920233
39	23.158006895344503	25.65942805757712	27.490283114250907	23.692281932827473
40	22.884370962274655	25.309626025037048	28.14000745854041	23.66599555414789
41	22.22481118008277	25.719377393363068	28.37295512171647	23.68285630483769
42	23.02417468674423	25.318232033201625	28.010566070432276	23.647027209621868
43	22.36028262833344	25.153312815517044	28.042062889429186	24.44434166672033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	229.0
1	232.0
2	235.0
3	603.0
4	971.0
5	971.0
6	1357.0
7	1743.0
8	1750.5
9	1758.0
10	2568.0
11	3378.0
12	3378.0
13	5816.0
14	8254.0
15	11436.0
16	14618.0
17	14722.0
18	14826.0
19	14826.0
20	17766.5
21	20707.0
22	20864.0
23	21021.0
24	23452.5
25	25884.0
26	25884.0
27	29165.5
28	32447.0
29	39819.5
30	47192.0
31	51001.0
32	54810.0
33	54810.0
34	60631.5
35	66453.0
36	71819.5
37	77186.0
38	86179.0
39	95172.0
40	95172.0
41	100253.0
42	105334.0
43	102837.5
44	100341.0
45	104799.5
46	109258.0
47	109258.0
48	113164.5
49	117071.0
50	118849.5
51	120628.0
52	124828.5
53	129029.0
54	129029.0
55	117910.0
56	106791.0
57	101710.0
58	96629.0
59	89302.5
60	81976.0
61	81976.0
62	75778.5
63	69581.0
64	62325.5
65	55070.0
66	48574.5
67	42079.0
68	42079.0
69	36454.0
70	30829.0
71	26766.5
72	22704.0
73	18359.0
74	14014.0
75	14014.0
76	11040.5
77	8067.0
78	6551.5
79	5036.0
80	3946.5
81	2857.0
82	2857.0
83	2194.5
84	1532.0
85	1318.5
86	1105.0
87	944.0
88	783.0
89	783.0
90	606.0
91	429.0
92	243.0
93	57.0
94	38.0
95	19.0
96	19.0
97	11.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1708109.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.898376432266105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.31445953578019	49.39568693317936
2	9.067877668773376	10.50030789416761
3	2.374100235006012	4.123696472829285
4	0.9840829683219641	2.279072245619475
5	0.5200514770317818	1.5055068090671044
6	0.3025594928566595	1.0510622046342242
7	0.21938028218699757	0.8891233511915684
8	0.14380017024930425	0.6660637110494537
9	0.11306247173902138	0.5891520194197478
>10	0.7399300655214626	8.3959286965939
>50	0.1072364294306822	4.397076172260022
>100	0.1060709023102292	12.120824892763
>500	0.006274995193504375	2.4693498557017435
>1k	9.108863990570866E-4	0.9733246583579961
>5k	2.0241919979046367E-4	0.6438240831655211
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5955	0.3486311470755087	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5032	0.2945947828856355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4377	0.25624828392099097	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2956	0.17305687166334233	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1716	0.10046197286004582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.8544273228464926E-5	0.0
2	0.0	0.0	0.0	7.61075551970044E-4	0.0
3	0.0	0.0	0.0	9.367083716554388E-4	0.0
4	0.0	0.0	0.0	0.001522151103940088	0.0
5	5.8544273228464926E-5	0.0	0.0	0.0018148724700824128	0.0
6	5.8544273228464926E-5	0.0	0.0	0.0018734167433108776	0.0
7	5.8544273228464926E-5	0.0	0.0	0.0021661381094532023	0.0
8	5.8544273228464926E-5	0.0	0.0	0.0022832266559101324	0.0
9	5.8544273228464926E-5	5.8544273228464926E-5	0.0	0.0036297449401648256	0.0
10	5.8544273228464926E-5	5.8544273228464926E-5	0.0	0.005327528863790308	0.0
11	5.8544273228464926E-5	5.8544273228464926E-5	0.0	0.005912971596074958	0.0
12	1.1708854645692985E-4	5.8544273228464926E-5	0.0	0.006615502874816537	0.0
13	1.1708854645692985E-4	5.8544273228464926E-5	0.0	0.0069082242409588615	0.0
14	1.1708854645692985E-4	5.8544273228464926E-5	0.0	0.007435122700015046	0.0
15	1.1708854645692985E-4	5.8544273228464926E-5	0.0	0.008254742525213556	0.0
16	1.1708854645692985E-4	5.8544273228464926E-5	0.0	0.009542716536239783	0.0
17	1.1708854645692985E-4	5.8544273228464926E-5	0.0	0.011591766099236055	0.0
18	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.012528474470891495	0.0
19	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.013816448481917723	0.0
20	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.015221511039400882	0.0
21	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.017153472055940224	0.0
22	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.020958849815790444	0.0
23	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.02640346722603768	0.0
24	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.03395567847250966	0.0
25	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.03694143640716137	0.0
26	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.04133225689929624	0.0
27	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.0515775047142776	0.0
28	1.1708854645692985E-4	1.1708854645692985E-4	0.0	0.07382432854109428	0.0
29	1.1708854645692985E-4	3.5126563937078957E-4	0.0	0.10426735061989603	0.0
30	1.1708854645692985E-4	3.5126563937078957E-4	5.8544273228464926E-5	0.15514232405543205	0.0
31	1.1708854645692985E-4	3.5126563937078957E-4	5.8544273228464926E-5	0.31555363270142595	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTAC	55	1.9033609E-5	23.545454	3
TAGTACT	135	5.638867E-11	19.185186	4
CCTAGTA	120	1.04204446E-7	16.958334	2
AAGACGG	265	0.0	16.754717	5
GCGCAAG	315	0.0	16.444445	1
ACGGACC	285	0.0	16.22807	8
CTAGTAG	80	3.3837702E-4	16.1875	3
CAAGACG	275	0.0	16.145454	4
GGTATCA	2930	0.0	16.100683	1
TACGGGT	70	0.0025931858	15.857142	4
CGCAAGA	335	0.0	15.462687	2
TCTAGCG	195	4.1836756E-11	15.179486	28
GACGGAC	295	0.0	15.050847	7
CGGACCA	305	0.0	14.557376	9
GTCGGTT	130	4.4467197E-6	14.230769	12
AGAGCGA	315	0.0	14.095239	15
AGCGTCA	105	1.6563751E-4	14.095238	3
GTCCTAT	225	2.7284841E-11	13.9777775	1
GCGAAAG	305	0.0	13.950819	18
GAGCGAA	320	0.0	13.875	16
>>END_MODULE