FastQCFastQC Report
Fri 10 Feb 2017
ERR1630797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838078
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95241.136409737518465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69600.8304716267459593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63200.7541064196888595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.5204766143485451No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13520.1613214999081231No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12200.14557117595259628No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.13936650287920696No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.13519028061827182No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.13328115044184433No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10610.1265991948243481No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10540.12576395037216107No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC9270.11061022959676783No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9030.10774653433212661No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT8930.10655332797185943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC250.00549550329.616
GCCGTCT1750.025.37142836
GGTATCA33000.021.6954561
TGCCGTC2100.021.14285735
ATGCCGT2150.020.65116334
AATGCGT450.003824956220.55555535
TATGCCG2200.020.18181833
CCGTATT555.1414967E-420.18181814
GACGGAC555.1414967E-420.1818187
ATACGGA555.1414967E-420.18181816
TAGTACT656.89928E-519.9230774
TTACTCG951.6747617E-719.47368419
TAATACG609.233968E-418.514
CGTATTA609.233968E-418.515
TAACGGC1002.8726208E-718.536
TGTGCGC500.00703332918.510
AGAACCG609.233968E-418.55
TATTGGG902.1508004E-618.52
GTGTAAC500.00703332918.51
TTAACGG1002.8726208E-718.535
Produced by FastQC (version 0.11.5)