##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630797.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	838078
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.921253153047807	33.0	31.0	34.0	30.0	34.0
2	32.06572657914896	33.0	31.0	34.0	30.0	34.0
3	32.14439944730682	34.0	31.0	34.0	30.0	34.0
4	35.75517911220674	37.0	35.0	37.0	33.0	37.0
5	35.75526502306468	37.0	35.0	37.0	35.0	37.0
6	35.813029336171574	37.0	35.0	37.0	35.0	37.0
7	35.78373373361429	37.0	35.0	37.0	35.0	37.0
8	35.79709764484929	37.0	35.0	37.0	35.0	37.0
9	37.47785886277888	39.0	37.0	39.0	35.0	39.0
10	37.40528685874107	39.0	37.0	39.0	34.0	39.0
11	37.53639995322631	39.0	37.0	39.0	35.0	39.0
12	37.46791110135334	39.0	37.0	39.0	35.0	39.0
13	37.51381971606462	39.0	37.0	39.0	35.0	39.0
14	38.717831753130376	40.0	38.0	41.0	35.0	41.0
15	38.69264197365877	40.0	38.0	41.0	35.0	41.0
16	38.63072172279907	40.0	38.0	41.0	34.0	41.0
17	38.67691551383046	40.0	38.0	41.0	35.0	41.0
18	38.68756726700856	40.0	38.0	41.0	35.0	41.0
19	38.748456587572996	40.0	38.0	41.0	35.0	41.0
20	38.738277344113556	40.0	38.0	41.0	35.0	41.0
21	38.66488441409988	40.0	38.0	41.0	34.0	41.0
22	38.57801421824699	40.0	38.0	41.0	34.0	41.0
23	38.537392700918055	40.0	38.0	41.0	34.0	41.0
24	38.48073568331348	40.0	38.0	41.0	34.0	41.0
25	38.42023892764158	40.0	38.0	41.0	34.0	41.0
26	38.203674359665804	40.0	37.0	41.0	34.0	41.0
27	38.0460911752844	40.0	37.0	41.0	33.0	41.0
28	37.93597493312078	40.0	37.0	41.0	33.0	41.0
29	37.81390992246545	40.0	37.0	41.0	33.0	41.0
30	37.68365116373416	40.0	37.0	41.0	33.0	41.0
31	37.574080216877185	40.0	36.0	41.0	33.0	41.0
32	37.41696715580173	40.0	36.0	41.0	32.0	41.0
33	37.326438589248255	40.0	36.0	41.0	32.0	41.0
34	37.20236660549495	39.0	35.0	41.0	32.0	41.0
35	37.09145449468904	39.0	35.0	41.0	31.0	41.0
36	36.99916833516689	39.0	35.0	41.0	31.0	41.0
37	36.74366228441744	39.0	35.0	41.0	31.0	41.0
38	36.56139404685483	39.0	35.0	41.0	30.0	41.0
39	36.419719882874865	39.0	35.0	41.0	30.0	41.0
40	36.23855655440186	39.0	35.0	41.0	30.0	41.0
41	36.029801522054036	39.0	35.0	41.0	29.0	41.0
42	35.83746381601713	39.0	35.0	41.0	27.0	41.0
43	35.00213464617852	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	3.0
11	7.0
12	2.0
13	1.0
14	3.0
15	2.0
16	7.0
17	18.0
18	24.0
19	65.0
20	130.0
21	333.0
22	520.0
23	921.0
24	1494.0
25	2420.0
26	3736.0
27	5438.0
28	7784.0
29	11035.0
30	14679.0
31	18444.0
32	23538.0
33	30298.0
34	39875.0
35	51420.0
36	71185.0
37	113481.0
38	182132.0
39	259079.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.34611098250998	20.71418173487432	12.685812060452605	22.25389522216309
2	19.545316784356586	21.78138550349729	34.001369800901585	24.671927911244538
3	22.271196714386967	24.517407687589937	29.042523488267204	24.168872109755892
4	14.20011025226769	16.21066296931789	34.26363655888831	35.325590219526106
5	13.31248404086493	39.08932104171688	33.30358272141734	14.29461219600085
6	33.03558857290133	34.12701443063772	16.168304143528406	16.669092852932543
7	27.384324609403897	32.370137385780325	21.391803626870054	18.853734377945727
8	28.603423547688877	31.35758246845759	20.292263965883844	19.74673001796969
9	27.00774868210358	13.149730693324488	22.33539121656934	37.5071294080026
10	19.112779478759734	27.483002775397996	31.868155470015918	21.536062275826353
11	33.04262849042691	22.6710401657125	21.006278651867728	23.28005269199287
12	21.653831743584725	28.52479124854727	27.86673794085992	21.954639067008085
13	29.91475733762251	20.529354069668933	26.97398094210801	22.58190765060054
14	22.634528051088324	22.776638928596146	27.193769553669227	27.395063466646302
15	26.742021625672074	26.632485281799546	25.16054591577395	21.464947176754432
16	24.166605017671387	26.22118704941545	26.901553316039795	22.71065461687337
17	22.082550788828726	27.218946207870864	27.178735153529864	23.519767849770545
18	21.67697994697391	24.17006531611616	30.02632213230749	24.126632604602435
19	22.035180496326117	25.845804328475392	30.30064027453292	21.81837490066557
20	23.304632743014373	24.345466651075437	30.733535542037853	21.616365063872337
21	23.886678805552705	25.321151491865912	29.308847147878836	21.483322554702546
22	24.186531563887847	24.952808688451434	29.5993928966039	21.261266851056824
23	23.02220079753913	24.938251570856174	29.721935189803332	22.31761244180136
24	21.91872355556404	26.151861759883925	29.472913022415575	22.45650166213646
25	22.368681674020795	26.014762349089228	29.848295743355628	21.76826023353435
26	22.12813127179093	26.68665685055568	29.220191915310984	21.965019962342407
27	22.37393178200597	25.944721135741545	29.734821818494222	21.946525263758264
28	21.51005037717253	26.108309727734174	29.896262639038373	22.485377256054925
29	21.191106317073114	26.216175582702327	30.608726156753907	21.983991943470656
30	20.74102887798033	27.423461778020663	30.3094700015989	21.52603934240011
31	22.221201367891773	27.387546266576617	28.822257594161883	21.568994771369727
32	21.18000949792263	26.794403384887804	29.51049902276399	22.51508809442558
33	20.723011461940295	26.372127653989246	30.065697942196312	22.839162941874143
34	21.612785444791534	26.032541123857207	30.25326998203031	22.101403449320948
35	20.86810535534879	26.59203558618649	30.30064027453292	22.239218783931804
36	21.117962767188732	27.62201131636912	28.972959557463625	22.287066358978517
37	21.790453871835318	26.154128851968434	29.69389484033706	22.36152243585919
38	21.124525402170207	26.038865117566623	29.66394536069435	23.17266411956882
39	21.100541954328833	26.383701755683838	30.087056336045094	22.428699953942235
40	20.329969286868288	25.95534067234792	30.621135502900685	23.093554537883108
41	20.288922988075093	26.120241791336845	30.523650543266857	23.067184677321205
42	20.350492436264883	26.33561553936507	30.74487100246039	22.569021021909656
43	19.885619238304788	25.172000696832512	30.41948362801553	24.522896436847166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	246.0
1	269.0
2	292.0
3	731.0
4	1170.0
5	1170.0
6	1708.5
7	2247.0
8	2266.0
9	2285.0
10	3311.0
11	4337.0
12	4337.0
13	7749.5
14	11162.0
15	14336.0
16	17510.0
17	16551.0
18	15592.0
19	15592.0
20	17898.0
21	20204.0
22	16451.5
23	12699.0
24	13216.5
25	13734.0
26	13734.0
27	14757.5
28	15781.0
29	17610.5
30	19440.0
31	22175.0
32	24910.0
33	24910.0
34	28581.5
35	32253.0
36	34642.0
37	37031.0
38	40565.0
39	44099.0
40	44099.0
41	46031.0
42	47963.0
43	49607.0
44	51251.0
45	51125.0
46	50999.0
47	50999.0
48	53264.0
49	55529.0
50	55518.0
51	55507.0
52	55569.5
53	55632.0
54	55632.0
55	53742.5
56	51853.0
57	48753.5
58	45654.0
59	42247.0
60	38840.0
61	38840.0
62	36092.5
63	33345.0
64	29252.5
65	25160.0
66	22591.0
67	20022.0
68	20022.0
69	16295.0
70	12568.0
71	10462.0
72	8356.0
73	6273.5
74	4191.0
75	4191.0
76	3207.0
77	2223.0
78	1781.0
79	1339.0
80	1076.5
81	814.0
82	814.0
83	710.0
84	606.0
85	575.0
86	544.0
87	456.0
88	368.0
89	368.0
90	314.0
91	260.0
92	154.0
93	48.0
94	29.5
95	11.0
96	11.0
97	6.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	838078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.296539010599844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.67693519007173	46.51961053856364
2	8.578855836952386	9.316043612347947
3	1.9984106201705387	3.2552034059186
4	0.8268791021256315	1.795866937024565
5	0.4942113624732757	1.3416983261005953
6	0.33323198119624753	1.0856005959960917
7	0.2634745652250598	1.0014029906330304
8	0.19214191322703678	0.8346112709682474
9	0.16089508911381759	0.7862441834414101
>10	1.2312933439184046	13.436658040827677
>50	0.15268037631067624	5.741514459715397
>100	0.08327954979135894	8.705644539198515
>500	0.005287590462069396	1.9606391478473324
>1k	0.001762530154023132	1.4913537679202373
>5k	6.609488077586745E-4	2.7279081834966785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9524	1.136409737518465	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6960	0.8304716267459593	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6320	0.7541064196888595	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4362	0.5204766143485451	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1352	0.1613214999081231	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1220	0.14557117595259628	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1168	0.13936650287920696	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1133	0.13519028061827182	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1117	0.13328115044184433	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1061	0.1265991948243481	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1054	0.12576395037216107	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	927	0.11061022959676783	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	903	0.10774653433212661	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	893	0.10655332797185943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.159238161603096E-4	0.0
3	0.0	0.0	0.0	0.0010738857242404646	0.0
4	0.0	0.0	0.0	0.0016704889043740558	0.0
5	0.0	0.0	0.0	0.002028450812454211	0.0
6	0.0	0.0	0.0	0.002028450812454211	0.0
7	0.0	0.0	0.0	0.0023864127205343653	0.0
8	0.0	0.0	0.0	0.00274437462861452	0.0
9	0.0	0.0	0.0	0.0051307873491488855	0.0
10	0.0	0.0	0.0	0.00763652070570997	0.0
11	0.0	0.0	0.0	0.008710406429950435	0.0
12	0.0	0.0	0.0	0.009545650882137461	0.0
13	0.0	0.0	0.0	0.010142254062271053	0.0
14	0.0	0.0	0.0	0.011454781058564954	0.0
15	0.0	0.0	0.0	0.01312526996293901	0.0
16	0.0	0.0	0.0	0.018256057312087896	0.0
17	0.0	0.0	0.0	0.024460730385477247	0.0
18	0.0	0.0	0.0	0.027921028830252076	0.0
19	0.0	0.0	0.0	0.03567687017198876	0.0
20	0.0	0.0	0.0	0.03854056543663	0.0
21	0.0	0.0	0.0	0.0456998035982331	0.0
22	0.0	0.0	0.0	0.06085352437362632	0.0
23	0.0	0.0	0.0	0.08018346740995468	0.0
24	0.0	0.0	0.0	0.11395120740551595	0.0
25	0.0	0.0	0.0	0.12111044556711906	0.0
26	0.0	0.0	0.0	0.1378153346108596	0.0
27	0.0	0.0	0.0	0.15941236973169562	0.0
28	0.0	0.0	0.0	0.19759497326024547	0.0
29	0.0	0.0	0.0	0.26107355162645957	0.0
30	0.0	0.0	0.0	0.35999035889260905	0.0
31	0.0	0.0	0.0	0.6550702917866833	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	25	0.005495503	29.6	16
GCCGTCT	175	0.0	25.371428	36
GGTATCA	3300	0.0	21.695456	1
TGCCGTC	210	0.0	21.142857	35
ATGCCGT	215	0.0	20.651163	34
AATGCGT	45	0.0038249562	20.555555	35
TATGCCG	220	0.0	20.181818	33
CCGTATT	55	5.1414967E-4	20.181818	14
GACGGAC	55	5.1414967E-4	20.181818	7
ATACGGA	55	5.1414967E-4	20.181818	16
TAGTACT	65	6.89928E-5	19.923077	4
TTACTCG	95	1.6747617E-7	19.473684	19
TAATACG	60	9.233968E-4	18.5	14
CGTATTA	60	9.233968E-4	18.5	15
TAACGGC	100	2.8726208E-7	18.5	36
TGTGCGC	50	0.007033329	18.5	10
AGAACCG	60	9.233968E-4	18.5	5
TATTGGG	90	2.1508004E-6	18.5	2
GTGTAAC	50	0.007033329	18.5	1
TTAACGG	100	2.8726208E-7	18.5	35
>>END_MODULE