FastQCFastQC Report
Fri 10 Feb 2017
ERR1630796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530197
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52100.3404790363593707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50460.3297614620862543No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG41220.2693770802060127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37940.24794193165977976No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT37210.24317130408699011No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG34580.2259839746124192No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC33320.217749740719659No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA32410.21180279401933214No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA26530.17337636918645116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25550.16697196504763767No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC24830.16226668853748896No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT23160.15135306107644964No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC23040.15056884832475817No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC22280.14560216756404568No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC22170.14488330587499518No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC21670.1416157527429475No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC21670.1416157527429475No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA21620.1412889974297427No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA20890.13651836985695306No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA20840.13619161454374829No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT20790.13586485923054353No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT20680.135145997541493No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC19780.12926440190380717No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA19590.12802273171362902No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG19350.12645430621024614No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC18910.12357885945404415No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG18620.12168367863745648No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC18230.11913498719445927No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT17610.11508322131072013No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA17450.11403760430846485No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG17350.1133840936820553No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA16970.11090075330169906No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG16750.10946302992359808No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT16580.10835206185870186No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC15310.10005247690330069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1054.799822E-717.61904728
GGTATCA21700.017.476961
CGGTTCT1502.5102054E-1017.26666612
GTATAGG752.0678306E-417.2666661
GTGTTAG1851.8189894E-1217.01
GCGTTAT1201.0418444E-716.9583321
GTATTAT1107.811759E-716.8181821
TAGTACT1107.811759E-716.8181824
CGCAAGA2000.016.6500022
CGCGGTT1708.54925E-1116.3235310
TTAGTAC803.3834815E-416.18753
TCGTTGG1059.347064E-615.8571422
CTATATA1059.347064E-615.8571422
TATCTCG1751.3096724E-1015.85714236
ACGGACC1751.3096724E-1015.8571428
GTCGTTG1059.347064E-615.8571421
CGGCCTT9300.015.71505424
CTAGCAC5100.015.5980393
ATACCGT957.06212E-515.5789486
ATCTCGC9400.015.54787211
Produced by FastQC (version 0.11.5)