##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630796.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1530197
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.968854990566573	33.0	31.0	34.0	30.0	34.0
2	32.16287379990943	33.0	31.0	34.0	30.0	34.0
3	32.25236750562183	34.0	31.0	34.0	30.0	34.0
4	35.855275497207224	37.0	35.0	37.0	35.0	37.0
5	35.81642363695655	37.0	35.0	37.0	35.0	37.0
6	35.888976386700534	37.0	35.0	37.0	35.0	37.0
7	35.843857359542596	37.0	35.0	37.0	35.0	37.0
8	35.84048524471032	37.0	35.0	37.0	35.0	37.0
9	37.53721841043996	39.0	37.0	39.0	35.0	39.0
10	37.451107929240486	39.0	37.0	39.0	35.0	39.0
11	37.53641589939073	39.0	37.0	39.0	35.0	39.0
12	37.483815482581655	39.0	37.0	39.0	35.0	39.0
13	37.521452466577834	39.0	37.0	39.0	35.0	39.0
14	38.810688427699176	40.0	38.0	41.0	35.0	41.0
15	38.8348081978987	40.0	38.0	41.0	35.0	41.0
16	38.79046880891807	40.0	38.0	41.0	35.0	41.0
17	38.77456954888815	40.0	38.0	41.0	35.0	41.0
18	38.747375011191366	40.0	38.0	41.0	35.0	41.0
19	38.836853686159365	40.0	38.0	41.0	35.0	41.0
20	38.79821813792603	40.0	38.0	41.0	35.0	41.0
21	38.752476315141124	40.0	38.0	41.0	35.0	41.0
22	38.70210633009998	40.0	38.0	41.0	34.0	41.0
23	38.654999977127126	40.0	38.0	41.0	34.0	41.0
24	38.60875103009612	40.0	38.0	41.0	34.0	41.0
25	38.56355619570552	40.0	38.0	41.0	34.0	41.0
26	38.4345858735836	40.0	38.0	41.0	34.0	41.0
27	38.30499798391972	40.0	38.0	41.0	34.0	41.0
28	38.2388287259745	40.0	38.0	41.0	34.0	41.0
29	38.18148643606019	40.0	38.0	41.0	34.0	41.0
30	38.102346299202	40.0	38.0	41.0	33.0	41.0
31	38.05067778854618	40.0	37.0	41.0	33.0	41.0
32	37.95955684137402	40.0	37.0	41.0	33.0	41.0
33	37.891000309110524	40.0	37.0	41.0	33.0	41.0
34	37.84643938002754	40.0	37.0	41.0	33.0	41.0
35	37.811815733529734	40.0	37.0	41.0	33.0	41.0
36	37.78325470511313	40.0	37.0	41.0	33.0	41.0
37	37.66572016544275	40.0	37.0	41.0	33.0	41.0
38	37.51719027027239	40.0	37.0	41.0	32.0	41.0
39	37.48263981696474	40.0	37.0	41.0	32.0	41.0
40	37.33606849314173	40.0	37.0	41.0	32.0	41.0
41	37.292654475208096	40.0	36.0	41.0	32.0	41.0
42	37.18729222446522	40.0	36.0	41.0	31.0	41.0
43	36.40557653687728	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	7.0
11	5.0
12	4.0
13	3.0
14	2.0
15	1.0
16	5.0
17	17.0
18	29.0
19	72.0
20	170.0
21	332.0
22	610.0
23	1132.0
24	1856.0
25	3078.0
26	4813.0
27	7328.0
28	10671.0
29	15469.0
30	21005.0
31	27692.0
32	35794.0
33	46548.0
34	62025.0
35	83155.0
36	117853.0
37	184755.0
38	357738.0
39	548023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82030352954555	19.79281099100312	12.885269020916915	24.501616458534425
2	17.492257532853614	21.854244910949376	36.837740500079406	23.81575705611761
3	19.688837450341364	24.15205362446796	31.491958224986718	24.667150700203962
4	14.539827224860591	15.933896093117422	34.98065935301141	34.54561732901058
5	13.318285161975876	38.61326352097148	34.843356770402764	13.225094546649876
6	33.75356244980222	35.388057877515116	16.318879203135282	14.539500469547384
7	27.27282826982408	32.07881076750249	22.07238675804488	18.575974204628555
8	25.56866860933592	35.74539748803585	20.691322751253598	17.994611151374627
9	25.597553779023226	14.989115780517148	20.480761627424442	38.93256881303518
10	15.992450645243716	28.603114500943345	34.01673117905734	21.387703674755603
11	33.941250701707034	22.664794140885128	22.826864776234693	20.56709038117314
12	20.196680558124218	27.04128945488718	29.960390720933315	22.801639266055286
13	28.6084732880799	20.83875474857159	26.920978148565183	23.631793814783325
14	21.871301538298663	22.132967193113043	26.0207019096234	29.975029358964893
15	24.625783477552236	29.10919313003489	24.02912827564033	22.235895116772546
16	24.18348748559826	27.966333746569887	24.695251657139572	23.154927110692284
17	22.8875105623655	28.063968234155475	26.12049298227614	22.92802822120289
18	21.291702963736043	26.606312781948994	28.479993098927785	23.62199115538718
19	23.223545726465286	27.15702618682431	28.325045729406085	21.294382357304322
20	23.47658504101106	25.83262155134274	28.834914720130804	21.8558786875154
21	22.78667387271051	27.498942946561783	26.899085542580465	22.815297638147243
22	22.992202964716306	26.50606425185777	27.204405707239005	23.29732707618692
23	22.142050990820135	27.337264417588063	27.571221221842677	22.949463369749125
24	24.221587155117934	26.976134445434152	27.089126432740358	21.713151966707557
25	23.36437726645654	26.300992617290454	27.589062061943658	22.74556805430935
26	23.40868528692711	26.542791549061985	27.199308324353005	22.8492148396579
27	23.197209248220982	26.340856765501435	27.163626644151044	23.298307342126538
28	22.11061712968984	26.915684712491267	27.9286915344887	23.0450066233302
29	22.56912018517877	27.674606602940667	27.251850578716336	22.504422633164225
30	21.98063386609698	28.36824278181175	27.251719876591054	22.39940347550021
31	22.841764818516832	27.09154442205807	27.71897997447387	22.347710784951218
32	21.89776871866825	27.32968369432171	27.510183329336023	23.26236425767401
33	22.281706211683854	26.719892928818968	27.831579855404236	23.166821004092938
34	22.28327463718724	26.928166765455693	27.62683497615013	23.161723621206942
35	22.85823328630235	26.824846735420344	27.386408416694056	22.93051156158325
36	22.437503145019893	27.253026897843874	27.622391103890543	22.687078853245694
37	23.36437726645654	25.819289934563983	27.263613769991707	23.55271902898777
38	22.36496346548843	26.493059390392215	28.56847843774364	22.573498706375712
39	22.425086443118108	26.101933280486104	28.59572983086491	22.87725044553087
40	22.137999224936397	25.395749697587956	29.4374515176804	23.028799559795242
41	21.33267808001192	26.63892296220683	29.303547190329088	22.724851767452165
42	22.380582369459617	25.259754136232132	29.005677046811616	23.35398644749663
43	21.204197890859806	25.86372865715983	28.946991792560045	23.985081659420324
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	203.0
2	216.0
3	568.5
4	921.0
5	921.0
6	1236.5
7	1552.0
8	1634.5
9	1717.0
10	2650.5
11	3584.0
12	3584.0
13	5626.5
14	7669.0
15	11694.5
16	15720.0
17	15644.0
18	15568.0
19	15568.0
20	17241.0
21	18914.0
22	20324.0
23	21734.0
24	23668.5
25	25603.0
26	25603.0
27	28524.0
28	31445.0
29	42687.5
30	53930.0
31	55071.0
32	56212.0
33	56212.0
34	60495.5
35	64779.0
36	66756.0
37	68733.0
38	81823.0
39	94913.0
40	94913.0
41	102118.5
42	109324.0
43	99929.0
44	90534.0
45	96429.0
46	102324.0
47	102324.0
48	107416.5
49	112509.0
50	115589.0
51	118669.0
52	124360.0
53	130051.0
54	130051.0
55	107987.0
56	85923.0
57	79639.5
58	73356.0
59	65401.5
60	57447.0
61	57447.0
62	52481.5
63	47516.0
64	42315.0
65	37114.0
66	32436.0
67	27758.0
68	27758.0
69	23657.0
70	19556.0
71	16747.5
72	13939.0
73	11339.0
74	8739.0
75	8739.0
76	6895.0
77	5051.0
78	4142.5
79	3234.0
80	2470.5
81	1707.0
82	1707.0
83	1292.5
84	878.0
85	736.0
86	594.0
87	483.5
88	373.0
89	373.0
90	275.0
91	177.0
92	98.5
93	20.0
94	11.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1530197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.11529245215108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.02542505931487	44.48330052679637
2	8.057740867870958	8.237475619297475
3	1.9809630339283228	3.0377251444844013
4	0.8199910330506519	1.6765632585010224
5	0.4478794292868982	1.1446744005651164
6	0.2897994014292524	0.8887908693908741
7	0.1886394307578204	0.6749651769835306
8	0.1364838912864436	0.5581131214491328
9	0.10492166015776666	0.48267912091765053
>10	0.6993491642291279	7.15213442160708
>50	0.10807183480689092	3.8866110738004305
>100	0.11205034753646126	12.602816753960944
>500	0.02023306126088558	6.985951917530338
>1k	0.008195670384156185	7.516872742850208
>5k	2.5611469950488077E-4	0.6713258518654419
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5210	0.3404790363593707	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5046	0.3297614620862543	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4122	0.2693770802060127	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3794	0.24794193165977976	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3721	0.24317130408699011	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3458	0.2259839746124192	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3332	0.217749740719659	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3241	0.21180279401933214	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2653	0.17337636918645116	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2555	0.16697196504763767	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2483	0.16226668853748896	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2316	0.15135306107644964	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	2304	0.15056884832475817	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2228	0.14560216756404568	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	2217	0.14488330587499518	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	2167	0.1416157527429475	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	2167	0.1416157527429475	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	2162	0.1412889974297427	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	2089	0.13651836985695306	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	2084	0.13619161454374829	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2079	0.13586485923054353	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	2068	0.135145997541493	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1978	0.12926440190380717	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	1959	0.12802273171362902	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1935	0.12645430621024614	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1891	0.12357885945404415	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1862	0.12168367863745648	No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC	1823	0.11913498719445927	No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT	1761	0.11508322131072013	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	1745	0.11403760430846485	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	1735	0.1133840936820553	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	1697	0.11090075330169906	No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG	1675	0.10946302992359808	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	1658	0.10835206185870186	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	1531	0.10005247690330069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.2675531320477036E-4	0.0
3	0.0	0.0	0.0	5.881595637685867E-4	0.0
4	0.0	0.0	0.0	9.802659396143112E-4	0.0
5	0.0	1.960531879228622E-4	0.0	9.802659396143112E-4	0.0
6	0.0	1.960531879228622E-4	0.0	0.0010456170022552652	0.0
7	0.0	1.960531879228622E-4	0.0	0.0011109680648962192	0.0
8	0.0	1.960531879228622E-4	0.0	0.0013723723154600354	0.0
9	0.0	2.614042505638163E-4	0.0	0.00320220206940675	0.0
10	0.0	2.614042505638163E-4	0.0	0.004313170134302969	0.0
11	0.0	2.614042505638163E-4	0.0	0.004639925447507739	0.0
12	0.0	2.614042505638163E-4	0.0	0.005032031823353464	0.0
13	0.0	2.614042505638163E-4	0.0	0.005685542449763005	0.0
14	0.0	2.614042505638163E-4	0.0	0.006208350950890637	0.0
15	0.0	2.614042505638163E-4	0.0	0.006665808389377316	0.0
16	0.0	2.614042505638163E-4	0.0	0.008364936018042122	0.0
17	0.0	2.614042505638163E-4	0.0	0.010652223210475514	0.0
18	0.0	2.614042505638163E-4	0.0	0.012220648713858412	0.0
19	0.0	2.614042505638163E-4	0.0	0.013854425279882263	0.0
20	0.0	2.614042505638163E-4	0.0	0.01555355290854707	0.0
21	0.0	2.614042505638163E-4	0.0	0.018886457103235728	0.0
22	0.0	2.614042505638163E-4	0.0	0.0240491910518711	0.0
23	0.0	3.921063758457244E-4	0.0	0.03117245687973509	0.0
24	0.0	3.921063758457244E-4	0.0	0.043066350280388736	0.0
25	0.0	3.921063758457244E-4	0.0	0.04607249916187262	0.0
26	0.0	3.921063758457244E-4	0.0	0.05201944586219944	0.0
27	0.0	3.921063758457244E-4	0.0	0.061495349945137785	0.0
28	0.0	3.921063758457244E-4	0.0	0.09031516856979853	0.0
29	0.0	4.5745743848667853E-4	0.0	0.12952580615437098	0.0
30	0.0	4.5745743848667853E-4	0.0	0.19200142203912307	0.0
31	0.0	4.5745743848667853E-4	0.0	0.42955253473899113	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	105	4.799822E-7	17.619047	28
GGTATCA	2170	0.0	17.47696	1
CGGTTCT	150	2.5102054E-10	17.266666	12
GTATAGG	75	2.0678306E-4	17.266666	1
GTGTTAG	185	1.8189894E-12	17.0	1
GCGTTAT	120	1.0418444E-7	16.958332	1
GTATTAT	110	7.811759E-7	16.818182	1
TAGTACT	110	7.811759E-7	16.818182	4
CGCAAGA	200	0.0	16.650002	2
CGCGGTT	170	8.54925E-11	16.32353	10
TTAGTAC	80	3.3834815E-4	16.1875	3
TCGTTGG	105	9.347064E-6	15.857142	2
CTATATA	105	9.347064E-6	15.857142	2
TATCTCG	175	1.3096724E-10	15.857142	36
ACGGACC	175	1.3096724E-10	15.857142	8
GTCGTTG	105	9.347064E-6	15.857142	1
CGGCCTT	930	0.0	15.715054	24
CTAGCAC	510	0.0	15.598039	3
ATACCGT	95	7.06212E-5	15.578948	6
ATCTCGC	940	0.0	15.547872	11
>>END_MODULE