FastQCFastQC Report
Fri 10 Feb 2017
ERR1630795.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630795.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339498
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110340.4716396423506239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108090.462022194504975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86110.3680704151061467No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64930.2775381727191047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA2150.019.79069715
AGTCGGT3150.017.61904711
CCGCTTA852.725068E-517.41176425
TACCGTC2250.017.2666667
TTATGCG1107.8161065E-716.8181824
TCGGTGA3450.016.62318813
GACGGAC3250.016.5076927
CAGTCGG3500.016.38571410
AAGACGG3400.016.323535
GAGCGAA3700.016.016
CGCCTTA2550.015.96078425
ATACCGT2100.015.8571446
CGGACCA3400.015.7794129
ACCGTCG2350.015.7446818
CGCTTAT957.064673E-515.57894826
ACGGACC3450.015.5507258
GCGAAAG3500.015.32857118
GGTATCA60000.015.2008341
CAAGACG4150.015.1566274
CGTCGTA2201.8189894E-1215.13636410
Produced by FastQC (version 0.11.5)