FastQCFastQC Report
Fri 10 Feb 2017
ERR1630794.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630794.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005583
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68840.6845780010203036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63350.6299828059941347No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47520.4725616880953636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.35959239565505785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGA450.003825479720.55555719
CTAGGTC500.00703428518.54
GTTATAG500.00703428518.51
TATCTAG1351.1514203E-917.8148161
TTAACGG650.00157974817.07692335
CAGTACT1452.9776857E-916.5862084
CGAACTA803.3820397E-416.187524
GCGAAAG1151.2417404E-616.08695618
TCACGTA700.002592191315.85714225
AAAGCCG1659.731593E-1015.6969715
TATACAA3350.014.9104485
GCGAACT750.004103931614.823
CACGTAG750.004103931614.826
GCGTATA750.004103931614.89
TAGACAA908.2727434E-414.3888895
TACGACG908.2727434E-414.3888895
TACAGCG908.2727434E-414.3888897
ATTATAC1304.4423396E-614.2307693
CTAGACA800.006297913.8754
TAGCGTA800.006297913.8757
Produced by FastQC (version 0.11.5)