##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630794.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1005583
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.207580080411066	31.0	31.0	34.0	28.0	34.0
2	31.39452039264785	31.0	31.0	34.0	28.0	34.0
3	31.470185951830928	31.0	31.0	34.0	28.0	34.0
4	35.26840051989741	37.0	35.0	37.0	33.0	37.0
5	34.976537988410705	35.0	35.0	37.0	32.0	37.0
6	35.06855028376574	36.0	35.0	37.0	32.0	37.0
7	35.00513632390365	36.0	35.0	37.0	32.0	37.0
8	35.01073307722982	36.0	35.0	37.0	32.0	37.0
9	36.58007543882504	38.0	35.0	39.0	32.0	39.0
10	36.31993679288532	38.0	35.0	39.0	32.0	39.0
11	36.517833933151216	38.0	35.0	39.0	32.0	39.0
12	36.4086355875149	38.0	35.0	39.0	32.0	39.0
13	36.51794431687887	38.0	35.0	39.0	32.0	39.0
14	37.54096479355757	39.0	37.0	40.0	32.0	41.0
15	37.515088262231956	39.0	36.0	40.0	32.0	41.0
16	37.44866211938746	39.0	36.0	40.0	32.0	41.0
17	37.428486758427695	39.0	36.0	40.0	32.0	41.0
18	37.40533103682142	39.0	36.0	40.0	32.0	41.0
19	37.47019788520689	39.0	36.0	40.0	32.0	41.0
20	37.42693740844863	39.0	36.0	40.0	32.0	41.0
21	37.38496971408625	39.0	36.0	40.0	32.0	41.0
22	37.2920236320622	39.0	36.0	40.0	32.0	41.0
23	37.21775328341867	39.0	36.0	40.0	32.0	41.0
24	37.129084322229	39.0	36.0	40.0	31.0	41.0
25	37.05457828941022	39.0	36.0	40.0	31.0	41.0
26	36.83230026760596	39.0	36.0	40.0	31.0	41.0
27	36.669078534541654	38.0	35.0	40.0	31.0	41.0
28	36.52729013915311	38.0	35.0	40.0	30.0	41.0
29	36.40121103877054	38.0	35.0	40.0	30.0	41.0
30	36.26794904050685	38.0	35.0	40.0	30.0	41.0
31	36.17359581456727	38.0	35.0	40.0	30.0	41.0
32	36.02835867352571	38.0	35.0	40.0	30.0	41.0
33	35.91516761918211	38.0	35.0	40.0	29.0	41.0
34	35.8218903859751	38.0	35.0	40.0	29.0	41.0
35	35.72924164390209	38.0	35.0	40.0	29.0	41.0
36	35.654033530797555	38.0	34.0	40.0	28.0	41.0
37	35.46776347651064	38.0	34.0	40.0	27.0	41.0
38	35.25379307327192	38.0	34.0	40.0	27.0	41.0
39	35.15434330134857	38.0	34.0	40.0	26.0	41.0
40	34.907941960037114	38.0	33.0	40.0	25.0	41.0
41	34.7904509125552	38.0	33.0	40.0	25.0	41.0
42	34.582838015360245	38.0	33.0	40.0	24.0	41.0
43	33.714451218845184	37.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	4.0
12	2.0
13	4.0
14	3.0
15	5.0
16	27.0
17	55.0
18	114.0
19	220.0
20	392.0
21	783.0
22	1340.0
23	2207.0
24	3423.0
25	5296.0
26	7799.0
27	10854.0
28	15192.0
29	20102.0
30	25861.0
31	32967.0
32	41682.0
33	52843.0
34	69794.0
35	93771.0
36	135703.0
37	191477.0
38	213440.0
39	80217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63956033465164	19.931621755737716	12.593788876701376	23.835029032909265
2	18.934090970113854	22.084005000084527	34.15670312644506	24.82520090335656
3	21.13022992632135	24.482215789248624	29.254074502055026	25.133479782375
4	15.129134044628836	16.18334836607222	33.366415303361336	35.32110228593761
5	14.202407956379535	38.023614162132816	33.505339688519	14.268638192968655
6	34.70096451511213	34.20055828310542	15.912759066133775	15.185718135648674
7	28.374087469656907	31.26146722846349	21.811029024953683	18.553416276925923
8	27.841660012152154	33.40917656722518	20.056226089740974	18.692937330881687
9	26.9702252325268	13.294476935270385	20.399509538247962	39.33578829395485
10	17.079445456019045	27.6380965072003	32.75701757090166	22.525440465878997
11	34.53409614124344	22.271557892287362	21.54600863379751	21.648337332671694
12	21.168913953398178	26.463255643740997	29.847163287366634	22.520667115494195
13	30.060969606685873	20.081584513660236	26.084768736146096	23.772677143507796
14	22.755655177145997	21.192780705322185	26.654985217530523	29.39657890000129
15	25.57431857937137	27.62099200165476	23.64996226069852	23.15472715827535
16	24.225051537267436	26.514071936379196	25.679431732636687	23.58144479371668
17	22.65253091987434	27.235245623682978	26.866603751256733	23.245619705185945
18	22.450757421316787	25.274094729127285	28.589683795370448	23.68546405418548
19	23.189135058965793	25.926054835851442	28.721050375752178	22.163759729430588
20	23.590991494486282	25.098276323287088	28.703150311809168	22.60758187041746
21	24.253791084375926	25.35375001367366	27.818389929026242	22.574068972924163
22	24.01850468832508	25.29915481864749	27.87318401365178	22.809156479375645
23	23.26222698673307	25.68420508302149	27.956419310986764	23.09714861925868
24	22.99273157959114	26.277293868333096	27.853195608915428	22.876778943160335
25	23.3009110138099	25.716027418920167	27.99311444206992	22.989947125200008
26	23.048719001812877	26.367888080844644	27.86662065687268	22.716772260469796
27	23.347550624861398	26.08486818094578	27.502056021233457	23.06552517295937
28	22.798714775408893	26.18123019183896	28.377369148046455	22.64268588470569
29	22.529716592265384	26.45341060857234	28.44121270944318	22.5756600897191
30	22.544036643419787	26.953021282181584	28.22014691974705	22.28279515465158
31	22.840978815274322	26.66463136309981	27.71695623334921	22.77743358827665
32	22.13014738713761	26.754827796412627	27.991622770074677	23.123402046375087
33	22.23734888119628	26.43739999582332	28.372198018462925	22.95305310451748
34	22.892590666309992	26.196544690990205	28.004451149233827	22.90641349346598
35	22.741335125991586	26.377335336814568	28.266488196399504	22.614841340794346
36	22.30228633538952	26.52391697154785	27.895161314381806	23.278635378680825
37	22.594156822460203	25.969512213313074	28.135618840016193	23.30071212421053
38	22.181659793373594	25.86211182965504	28.546126973109132	23.41010140386224
39	22.262011191517754	25.842322314518047	28.639704529611183	23.255961964353016
40	21.99311245317393	25.864299615248072	29.030124813168083	23.112463118409917
41	21.455812200484694	25.94077266620458	29.32129918664098	23.282115946669744
42	21.669021851005834	25.919491479072338	29.04205818913009	23.36942848079174
43	21.359847968790245	25.596295880101394	29.06154936986803	23.982306781240332
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	251.0
2	253.0
3	620.0
4	987.0
5	987.0
6	1543.5
7	2100.0
8	2200.0
9	2300.0
10	3203.0
11	4106.0
12	4106.0
13	7098.5
14	10091.0
15	14070.5
16	18050.0
17	16308.0
18	14566.0
19	14566.0
20	16294.5
21	18023.0
22	15631.5
23	13240.0
24	14577.0
25	15914.0
26	15914.0
27	17726.0
28	19538.0
29	22175.0
30	24812.0
31	27750.5
32	30689.0
33	30689.0
34	33731.5
35	36774.0
36	39868.0
37	42962.0
38	46993.5
39	51025.0
40	51025.0
41	53295.5
42	55566.0
43	57274.5
44	58983.0
45	61473.5
46	63964.0
47	63964.0
48	64845.5
49	65727.0
50	66079.5
51	66432.0
52	67405.0
53	68378.0
54	68378.0
55	65706.5
56	63035.0
57	60826.5
58	58618.0
59	54622.0
60	50626.0
61	50626.0
62	46855.5
63	43085.0
64	37847.0
65	32609.0
66	28336.5
67	24064.0
68	24064.0
69	20812.0
70	17560.0
71	15098.5
72	12637.0
73	10133.0
74	7629.0
75	7629.0
76	6119.5
77	4610.0
78	3752.5
79	2895.0
80	2223.5
81	1552.0
82	1552.0
83	1178.5
84	805.0
85	668.0
86	531.0
87	450.5
88	370.0
89	370.0
90	284.0
91	198.0
92	110.0
93	22.0
94	14.5
95	7.0
96	7.0
97	3.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1005583.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.48866520046765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34847739337333	60.50869289165831
2	7.1673197714869294	9.817603284281212
3	1.8613194490224	3.8243785372564223
4	0.7726555961103966	2.1167260174909086
5	0.4288266455461074	1.4684882277923483
6	0.25904111086714326	1.06448279492022
7	0.18082050960758142	0.8668908740724112
8	0.13143413177757926	0.7201398597783017
9	0.09065106285783447	0.5587713264723096
>10	0.646688554380556	8.615593639566292
>50	0.07470155041457936	3.5767339504250524
>100	0.03646610872591889	4.179506495531869
>500	0.0010169828007972335	0.5340302591358913
>1k	2.9056651451349525E-4	0.8326374526642703
>5k	2.9056651451349525E-4	1.315324388954229
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6884	0.6845780010203036	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6335	0.6299828059941347	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4752	0.4725616880953636	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3616	0.35959239565505785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.944479968336776E-5	0.0
2	0.0	0.0	0.0	8.950031971503099E-4	0.0
3	0.0	0.0	0.0	0.001193337596200413	0.0
4	0.0	0.0	0.0	0.0017900063943006197	0.0
5	0.0	0.0	0.0	0.0018894511939839874	0.0
6	0.0	0.0	0.0	0.001988895993667355	0.0
7	0.0	0.0	0.0	0.002187785593034091	0.0
8	0.0	0.0	0.0	0.0022872303927174583	0.0
9	0.0	0.0	0.0	0.0033811231892345037	0.0
10	0.0	0.0	0.0	0.004972239984168388	0.0
11	0.0	0.0	0.0	0.005668353581951962	0.0
12	0.0	0.0	0.0	0.0076572495756193175	0.0
13	0.0	0.0	0.0	0.008353363173402892	0.0
14	0.0	0.0	0.0	0.009845035168653407	0.0
15	0.0	0.0	0.0	0.012331155160737602	0.0
16	0.0	0.0	0.0	0.018496732741106404	0.0
17	0.0	0.0	0.0	0.026452316715775824	0.0
18	0.0	0.0	0.0	0.029535105505960224	0.0
19	0.0	0.0	0.0	0.035700683086329024	0.0
20	0.0	0.0	0.0	0.0391812510752469	0.0
21	0.0	0.0	0.0	0.04743516944896642	0.0
22	0.0	0.0	0.0	0.06523578859228925	0.0
23	0.0	0.0	0.0	0.09397533570078254	0.0
24	0.0	0.0	0.0	0.1388249403579814	0.0
25	0.0	0.0	0.0	0.14727774833106766	0.0
26	0.0	0.0	0.0	0.16676892906900773	0.0
27	0.0	0.0	0.0	0.1899395673952324	0.0
28	0.0	0.0	0.0	0.22782803607459554	0.0
29	0.0	0.0	0.0	0.2854065750912655	0.0
30	0.0	0.0	0.0	0.3846524851752665	0.0
31	0.0	0.0	0.0	0.6834841082237866	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGA	45	0.0038254797	20.555557	19
CTAGGTC	50	0.007034285	18.5	4
GTTATAG	50	0.007034285	18.5	1
TATCTAG	135	1.1514203E-9	17.814816	1
TTAACGG	65	0.001579748	17.076923	35
CAGTACT	145	2.9776857E-9	16.586208	4
CGAACTA	80	3.3820397E-4	16.1875	24
GCGAAAG	115	1.2417404E-6	16.086956	18
TCACGTA	70	0.0025921913	15.857142	25
AAAGCCG	165	9.731593E-10	15.69697	15
TATACAA	335	0.0	14.910448	5
GCGAACT	75	0.0041039316	14.8	23
CACGTAG	75	0.0041039316	14.8	26
GCGTATA	75	0.0041039316	14.8	9
TAGACAA	90	8.2727434E-4	14.388889	5
TACGACG	90	8.2727434E-4	14.388889	5
TACAGCG	90	8.2727434E-4	14.388889	7
ATTATAC	130	4.4423396E-6	14.230769	3
CTAGACA	80	0.0062979	13.875	4
TAGCGTA	80	0.0062979	13.875	7
>>END_MODULE