FastQCFastQC Report
Fri 10 Feb 2017
ERR1630793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726884
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG43510.25195670351917093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36830.21327431373502795No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG33640.19480173538002551No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT33190.1921958857688183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32500.18820024969830051No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA25890.14992321429812308No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC25770.14922832106846784No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24890.14413243738432924No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT24260.14048424792863912No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC21550.12479124249225773No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC19680.11396248966346322No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA19180.11106710120656628No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC19100.11060383905346276No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC18270.10579749421501386No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC18180.10527632429277241No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT18020.1043497999865654No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC17640.10214930475932374No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG17400.1007595183000132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG250.00549709829.63
GGTATCA18550.018.5498661
TGTACCG1401.87174E-917.1785725
TTACACT2250.016.4444454
CATACCG700.00259320115.8571435
CTAGCAC3450.015.5507253
GCATTAG1201.9367726E-615.4166671
ACGCGTA1101.4526289E-515.13636427
CATATAG1353.9752013E-715.0740753
CTTATAC6750.015.07407437
TAGACAG1853.0559022E-1015.05
TCGCCAT6950.014.64028713
CTATGCT1405.9993727E-714.5357154
GTCCTAC1405.9993727E-714.5357151
GTATTAC908.276983E-414.3888881
AAGACGG2201.8189894E-1114.2954545
GTCTATA1304.446787E-614.2307691
ATGGTCG1458.9166497E-714.03448336
GCTTAGG11600.013.8751
TAGCACT4300.013.7674424
Produced by FastQC (version 0.11.5)