##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630793.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1726884
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.735115966098476	31.0	31.0	34.0	30.0	34.0
2	31.936136995883917	33.0	31.0	34.0	30.0	34.0
3	32.02313994454752	33.0	31.0	34.0	30.0	34.0
4	35.69836537949278	37.0	35.0	37.0	33.0	37.0
5	35.5974755687122	37.0	35.0	37.0	33.0	37.0
6	35.670204831361	37.0	35.0	37.0	33.0	37.0
7	35.62956805436845	37.0	35.0	37.0	33.0	37.0
8	35.62160631518967	37.0	35.0	37.0	33.0	37.0
9	37.288344208412376	39.0	37.0	39.0	34.0	39.0
10	37.13901976044714	39.0	37.0	39.0	33.0	39.0
11	37.25269444849799	39.0	37.0	39.0	34.0	39.0
12	37.183646961810986	39.0	37.0	39.0	34.0	39.0
13	37.23109774599799	39.0	37.0	39.0	34.0	39.0
14	38.46200729174629	40.0	38.0	41.0	34.0	41.0
15	38.475130350388326	40.0	38.0	41.0	34.0	41.0
16	38.42921006853963	40.0	38.0	41.0	34.0	41.0
17	38.394277206807175	40.0	38.0	41.0	34.0	41.0
18	38.35863497490277	40.0	38.0	41.0	34.0	41.0
19	38.42953145665835	40.0	38.0	41.0	34.0	41.0
20	38.382177957523496	40.0	38.0	41.0	34.0	41.0
21	38.34244627896257	40.0	38.0	41.0	34.0	41.0
22	38.28380597654504	40.0	38.0	41.0	34.0	41.0
23	38.22277639957287	40.0	38.0	41.0	34.0	41.0
24	38.17037797559072	40.0	38.0	41.0	33.0	41.0
25	38.11884411460179	40.0	38.0	41.0	33.0	41.0
26	37.98105836871498	40.0	37.0	41.0	33.0	41.0
27	37.848959744835206	40.0	37.0	41.0	33.0	41.0
28	37.76563220227879	40.0	37.0	41.0	33.0	41.0
29	37.69516539617021	40.0	37.0	41.0	33.0	41.0
30	37.61212391799334	40.0	37.0	41.0	32.0	41.0
31	37.58450133303685	40.0	37.0	41.0	32.0	41.0
32	37.490573773339726	39.0	37.0	41.0	32.0	41.0
33	37.43146615522525	39.0	37.0	41.0	32.0	41.0
34	37.38314849173425	39.0	36.0	41.0	32.0	41.0
35	37.34937725985069	39.0	36.0	41.0	32.0	41.0
36	37.33303684555535	39.0	36.0	41.0	32.0	41.0
37	37.209615700880896	39.0	36.0	41.0	31.0	41.0
38	37.06894730624639	39.0	36.0	40.0	31.0	41.0
39	37.03512569460369	39.0	36.0	40.0	31.0	41.0
40	36.88384106865313	39.0	36.0	40.0	31.0	41.0
41	36.84519400260817	39.0	36.0	40.0	31.0	41.0
42	36.737716024932766	39.0	35.0	40.0	30.0	41.0
43	35.90562597140283	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	7.0
11	3.0
12	2.0
13	1.0
14	0.0
15	3.0
16	16.0
17	30.0
18	81.0
19	141.0
20	238.0
21	521.0
22	929.0
23	1665.0
24	2670.0
25	4468.0
26	6757.0
27	10065.0
28	14320.0
29	19973.0
30	27224.0
31	36027.0
32	46577.0
33	60764.0
34	81163.0
35	110069.0
36	158377.0
37	254653.0
38	481869.0
39	408267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98690589524253	19.30523416743684	12.442758170207147	25.265101767113485
2	18.549132425802775	21.408096895911942	35.31910655261152	24.723664125673757
3	20.300900349994556	23.58803486510964	30.29381243905207	25.817252345843727
4	15.058683733244388	15.659129391435672	34.22546042467241	35.056726450647524
5	14.135054815494266	37.544270489505955	34.55223396591781	13.768440729081977
6	35.090428772285804	34.65339883860178	15.759715186428272	14.496457202684141
7	28.65704934436824	30.885108669719564	21.579445984791104	18.878396001121097
8	26.36494402635035	34.84889546721146	20.212706817597475	18.57345368884071
9	26.34849821991518	14.350703347763949	19.408599535348063	39.892198896972815
10	16.31261856615731	27.65831405004621	33.49518554807387	22.533881835722607
11	35.25453939002272	21.870200893632692	21.840899562448897	21.034360153895687
12	21.06661478130552	25.970823749597542	29.132530036759853	23.830031432337087
13	29.487446753806278	20.17715144734678	25.968854885446852	24.36654691340009
14	22.65519861206659	21.148843813481392	24.920897987357577	31.275059587094447
15	25.35682767342798	28.24376159603077	22.83633411393006	23.56307661661119
16	25.352079236358666	26.914720386545945	23.64739032847603	24.085810048619365
17	23.940229917006587	26.98890023881164	25.05188536114759	24.01898448303418
18	22.732042221712632	25.738903134200097	27.099040815712	24.43001382837527
19	24.464874305396307	25.95223535570426	26.65540939634625	22.927480942553178
20	24.60692206309167	24.95616381876258	27.109580029695103	23.327334088450645
21	24.167286279796443	26.240326507165506	25.560547205255247	24.031840007782804
22	24.338345829829912	25.47414881370144	25.783202577590618	24.404302778878026
23	23.534180639811357	26.264821493510855	26.098973642699796	24.102024223977985
24	24.913022530754816	26.149121770773252	25.75453823186734	23.18331746660459
25	24.55607904178856	25.465810094945578	26.068977418286348	23.909133444979513
26	24.452829489415617	25.932083452044257	25.751353304564756	23.863733753975367
27	24.63963995265461	25.2410121351521	25.732127925210957	24.387219986982334
28	23.66111446976172	25.66802402477526	26.506007351970368	24.164854153492648
29	23.679818679193275	26.33274730670966	26.049115053472033	23.938318960625033
30	23.176715980922864	27.130021472200795	26.034638111187547	23.65862443568879
31	24.194445023522135	26.067298092981346	26.080095709960833	23.658161173535685
32	23.214008584247697	26.157286766221706	26.219190171430164	24.409514478100437
33	23.495556157796354	25.751411212333892	26.390075998156213	24.362956631713537
34	23.84566653000433	25.68875500612664	26.411849319352083	24.053729144516943
35	24.1482925315192	25.728653459062684	26.36407540981328	23.758978599604838
36	23.638993701951026	26.31473799050776	26.174195834809982	23.872072472731233
37	24.777634166510314	24.996583441620864	26.0034258236222	24.22235656824662
38	23.84705631646364	25.596160483275078	26.873432147150588	23.68335105311069
39	23.963335116892623	25.392730490293502	26.737059350830744	23.906875041983135
40	23.46567574892118	24.77305945274842	27.535028409551536	24.22623638877886
41	22.77622584956488	25.699467943417158	27.68784701230656	23.8364591947114
42	23.8645444627433	24.772422467287903	27.301023114465128	24.062009955503672
43	22.964368191494046	24.80085518193463	27.40971599713704	24.825060629434287
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	91.0
1	102.0
2	113.0
3	330.0
4	547.0
5	547.0
6	748.5
7	950.0
8	988.0
9	1026.0
10	1571.0
11	2116.0
12	2116.0
13	3537.5
14	4959.0
15	7498.5
16	10038.0
17	10254.5
18	10471.0
19	10471.0
20	12377.0
21	14283.0
22	15550.0
23	16817.0
24	19262.0
25	21707.0
26	21707.0
27	24823.5
28	27940.0
29	37789.5
30	47639.0
31	50505.0
32	53371.0
33	53371.0
34	59350.0
35	65329.0
36	69546.5
37	73764.0
38	86779.0
39	99794.0
40	99794.0
41	106789.0
42	113784.0
43	107601.5
44	101419.0
45	108548.0
46	115677.0
47	115677.0
48	122073.0
49	128469.0
50	132243.5
51	136018.0
52	142854.5
53	149691.0
54	149691.0
55	130126.0
56	110561.0
57	103144.0
58	95727.0
59	86802.5
60	77878.0
61	77878.0
62	73179.5
63	68481.0
64	61445.5
65	54410.0
66	47849.0
67	41288.0
68	41288.0
69	35246.5
70	29205.0
71	25525.5
72	21846.0
73	17427.5
74	13009.0
75	13009.0
76	10397.5
77	7786.0
78	6377.5
79	4969.0
80	3770.5
81	2572.0
82	2572.0
83	2005.0
84	1438.0
85	1151.5
86	865.0
87	692.5
88	520.0
89	520.0
90	388.0
91	256.0
92	151.5
93	47.0
94	28.5
95	10.0
96	10.0
97	6.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1726884.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.56384509634259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.15321340711057	45.28544164083505
2	8.278253681694691	8.702736883856549
3	2.199693756587101	3.468730856419088
4	0.9437271576403875	1.9842371250968402
5	0.533155764543316	1.4012358509838483
6	0.33850528423139287	1.0675883594779412
7	0.22735984160495654	0.8365635246676346
8	0.16836336128575302	0.7079860514019108
9	0.12902728392798	0.6103953149032939
>10	0.7783901616430293	7.89889657352147
>50	0.10786828957277075	4.013552480006202
>100	0.12166711689566292	13.690843038857675
>500	0.014807636687035813	5.067150721195053
>1k	0.005967256575372641	5.264641578777509
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4351	0.25195670351917093	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3683	0.21327431373502795	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3364	0.19480173538002551	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3319	0.1921958857688183	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3250	0.18820024969830051	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2589	0.14992321429812308	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2577	0.14922832106846784	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2489	0.14413243738432924	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2426	0.14048424792863912	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2155	0.12479124249225773	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1968	0.11396248966346322	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1918	0.11106710120656628	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	1910	0.11060383905346276	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1827	0.10579749421501386	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	1818	0.10527632429277241	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1802	0.1043497999865654	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1764	0.10214930475932374	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1740	0.1007595183000132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.895388456896931E-4	5.790776913793862E-5
3	0.0	0.0	0.0	6.948932296552635E-4	5.790776913793862E-5
4	0.0	0.0	0.0	0.0011581553827587725	5.790776913793862E-5
5	0.0	5.790776913793862E-5	0.0	0.0012739709210346497	5.790776913793862E-5
6	0.0	5.790776913793862E-5	0.0	0.0012739709210346497	5.790776913793862E-5
7	0.0	5.790776913793862E-5	0.0	0.0017372330741381586	5.790776913793862E-5
8	0.0	5.790776913793862E-5	0.0	0.001968864150689913	5.790776913793862E-5
9	5.790776913793862E-5	5.790776913793862E-5	0.0	0.0034744661482763173	5.790776913793862E-5
10	5.790776913793862E-5	5.790776913793862E-5	0.0	0.00503797591500066	5.790776913793862E-5
11	5.790776913793862E-5	5.790776913793862E-5	0.0	0.005501238068104169	5.790776913793862E-5
12	5.790776913793862E-5	5.790776913793862E-5	0.0	0.005964500221207678	1.7372330741381587E-4
13	5.790776913793862E-5	5.790776913793862E-5	0.0	0.006254039066897372	1.7372330741381587E-4
14	5.790776913793862E-5	5.790776913793862E-5	0.0	0.006601485681725003	1.7372330741381587E-4
15	5.790776913793862E-5	1.1581553827587724E-4	0.0	0.007006840065690573	1.7372330741381587E-4
16	5.790776913793862E-5	1.1581553827587724E-4	0.0	0.00845453429413904	1.7372330741381587E-4
17	5.790776913793862E-5	1.1581553827587724E-4	0.0	0.009960136291725444	1.7372330741381587E-4
18	5.790776913793862E-5	1.1581553827587724E-4	0.0	0.010307582906553075	1.7372330741381587E-4
19	5.790776913793862E-5	1.7372330741381587E-4	0.0	0.01134992275103597	1.7372330741381587E-4
20	5.790776913793862E-5	1.7372330741381587E-4	0.0	0.012681801441208559	1.7372330741381587E-4
21	1.1581553827587724E-4	1.7372330741381587E-4	0.0	0.014245311207932902	1.7372330741381587E-4
22	1.1581553827587724E-4	1.7372330741381587E-4	0.0	0.016851160819140138	1.7372330741381587E-4
23	1.1581553827587724E-4	1.7372330741381587E-4	0.0	0.02131005904276141	1.7372330741381587E-4
24	1.1581553827587724E-4	1.7372330741381587E-4	0.0	0.027100835956555276	1.7372330741381587E-4
25	1.1581553827587724E-4	1.7372330741381587E-4	0.0	0.028838069030693435	1.7372330741381587E-4
26	1.1581553827587724E-4	1.7372330741381587E-4	0.0	0.03271788956293532	1.7372330741381587E-4
27	1.1581553827587724E-4	1.7372330741381587E-4	0.0	0.038682389784143	1.7372330741381587E-4
28	1.1581553827587724E-4	1.7372330741381587E-4	0.0	0.05281188545380002	1.7372330741381587E-4
29	1.1581553827587724E-4	1.7372330741381587E-4	0.0	0.07545382318673402	1.7372330741381587E-4
30	1.7372330741381587E-4	1.7372330741381587E-4	0.0	0.10932986813242812	1.7372330741381587E-4
31	1.7372330741381587E-4	1.7372330741381587E-4	0.0	0.24176493615089376	1.7372330741381587E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATCG	25	0.005497098	29.6	3
GGTATCA	1855	0.0	18.549866	1
TGTACCG	140	1.87174E-9	17.178572	5
TTACACT	225	0.0	16.444445	4
CATACCG	70	0.002593201	15.857143	5
CTAGCAC	345	0.0	15.550725	3
GCATTAG	120	1.9367726E-6	15.416667	1
ACGCGTA	110	1.4526289E-5	15.136364	27
CATATAG	135	3.9752013E-7	15.074075	3
CTTATAC	675	0.0	15.074074	37
TAGACAG	185	3.0559022E-10	15.0	5
TCGCCAT	695	0.0	14.640287	13
CTATGCT	140	5.9993727E-7	14.535715	4
GTCCTAC	140	5.9993727E-7	14.535715	1
GTATTAC	90	8.276983E-4	14.388888	1
AAGACGG	220	1.8189894E-11	14.295454	5
GTCTATA	130	4.446787E-6	14.230769	1
ATGGTCG	145	8.9166497E-7	14.034483	36
GCTTAGG	1160	0.0	13.875	1
TAGCACT	430	0.0	13.767442	4
>>END_MODULE