FastQCFastQC Report
Fri 10 Feb 2017
ERR1630792.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630792.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462821
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72060.49260982717639407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70990.485295193328507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51040.3489148706506128No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37770.2581997387240134No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT16390.11204378389427004No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG15330.10479751111038194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGC400.001931437323.1250023
CCGCTTA450.003826300420.55555525
CTTACAC1850.020.03
CTAGTAC759.268604E-619.7333343
ATACCGT1156.4084816E-817.6956526
TTTTCGG1705.456968E-1217.41176429
CGCGTAA1502.5102054E-1017.26666610
GCGTAAC1502.5102054E-1017.26666611
TTTCGGA1757.2759576E-1216.91428630
AAGACGG1655.4569682E-1116.8181825
ATGGTCG1005.879994E-616.65000236
GCAGTCG1452.9813236E-916.5862069
GTTTTCG1901.8189894E-1216.55263128
CCGGTCG1352.2206223E-816.44444520
GTCGCGT1708.54925E-1116.323538
CGCGGTT1708.54925E-1116.3235310
TACCGTC1251.6585E-716.2799997
TCGCCAT4250.016.10588313
TCGTTTA1059.346504E-615.85714230
TCGCGTA1659.749783E-1015.696979
Produced by FastQC (version 0.11.5)