FastQCFastQC Report
Fri 10 Feb 2017
ERR1630791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660376
Sequences flagged as poor quality0
Sequence length43
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT238083.605218845021624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT228323.4574242552727537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT189282.8662458962772726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145172.198293093631507No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43340.6562927786594304No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36810.5574097180999915No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34000.5148582019940156No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.42475801664506285No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA27030.4093122705852424No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA24850.37630077410444956No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20020.3031606236447115No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT18960.2871091620531334No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.28135486450143554No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.25000908573297637No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16050.2430433571177632No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA15040.22774903994088216No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA13320.20170327207530256No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.19352611239657405No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11380.1723260687850558No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA11070.16763177341393387No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA10810.16369462245750904No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT9870.14946030746120392No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA9680.14658315868535501No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA9520.14416029655832435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9400.14234314996305136No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA9190.1391631434213236No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8930.13522599246489878No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC8600.13022883932789805No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA8070.12220310853210897No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA7720.11690309762922942No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA7530.11402594885338051No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA7410.1122088022581075No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA7320.11084594231165275No Hit
GGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTC6990.10584878917465203No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC6960.10539450252583377No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT6850.10372878481350019No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA6780.10266878263292427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAGT303.5978353E-430.8333321
TAGTTCC250.0054946729.64
ATTGAGT454.0048617E-628.7777771
TGGCCAC358.865384E-426.4285722
GTTATAC358.865384E-426.428573
TGAGTAT451.3223794E-424.6666663
TTGAGTA451.3223794E-424.6666662
GTATAGA451.3223794E-424.6666661
TTGGTAA751.371518E-824.66666433
GTAAACA751.371518E-824.66666436
GCAGCGT851.9736035E-923.9411771
ATACACT851.9736035E-923.9411774
TACACTG952.8012437E-1023.3684225
TCCTGTG400.001930321323.1250027
ACAGGTA400.001930321323.12500224
ATTGGCC400.001930321323.12500220
CTAGAGG903.8216967E-922.6111118
TGCTAGA903.8216967E-922.6111116
CTCTAAT903.8216967E-922.611111
AATACTG903.8216967E-922.611115
Produced by FastQC (version 0.11.5)