##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630791.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660376
Sequences flagged as poor quality	0
Sequence length	43
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955678280252464	33.0	31.0	34.0	30.0	34.0
2	31.97960404375689	33.0	31.0	34.0	30.0	34.0
3	31.98670908694441	33.0	31.0	34.0	30.0	34.0
4	35.66234084824403	37.0	35.0	37.0	33.0	37.0
5	35.692102680896944	37.0	35.0	37.0	35.0	37.0
6	35.86589912413534	37.0	35.0	37.0	35.0	37.0
7	35.75712775751996	37.0	35.0	37.0	35.0	37.0
8	35.870407161980445	37.0	35.0	37.0	35.0	37.0
9	37.425848304602226	39.0	37.0	39.0	34.0	39.0
10	37.39091214701927	39.0	37.0	39.0	34.0	39.0
11	37.54700503955323	39.0	37.0	39.0	35.0	39.0
12	37.55830769137582	39.0	37.0	39.0	35.0	39.0
13	37.5992056040801	39.0	37.0	39.0	35.0	39.0
14	38.68399214992671	40.0	38.0	41.0	34.0	41.0
15	38.60034889214629	40.0	38.0	41.0	34.0	41.0
16	38.479811501326516	40.0	38.0	41.0	34.0	41.0
17	38.704737906889406	40.0	38.0	41.0	35.0	41.0
18	38.810797182211346	40.0	38.0	41.0	35.0	41.0
19	38.96925690818564	40.0	38.0	41.0	35.0	41.0
20	38.97025785310187	40.0	39.0	41.0	35.0	41.0
21	38.92204289677396	40.0	38.0	41.0	35.0	41.0
22	38.76765055059542	40.0	38.0	41.0	35.0	41.0
23	38.62237573745866	40.0	38.0	41.0	35.0	41.0
24	38.49187432614147	40.0	38.0	41.0	35.0	41.0
25	38.36781167092687	40.0	37.0	41.0	34.0	41.0
26	37.98380165239197	40.0	36.0	41.0	34.0	41.0
27	37.66842071789405	40.0	35.0	41.0	33.0	41.0
28	37.436327183301636	39.0	35.0	41.0	33.0	41.0
29	37.15932589918471	39.0	35.0	41.0	33.0	41.0
30	36.793537318133914	38.0	35.0	41.0	33.0	41.0
31	36.37343119677275	38.0	35.0	40.0	32.0	41.0
32	35.95800725647207	37.0	35.0	40.0	31.0	41.0
33	35.561160611530404	37.0	35.0	40.0	30.0	41.0
34	35.22468260506136	36.0	35.0	40.0	29.0	41.0
35	34.82563569845058	36.0	35.0	40.0	25.0	41.0
36	34.39365906695579	35.0	35.0	40.0	22.0	41.0
37	33.83065859449768	35.0	34.0	40.0	19.0	41.0
38	33.254538323621695	35.0	33.0	40.0	15.0	41.0
39	32.70863265775861	35.0	33.0	40.0	10.0	41.0
40	32.03494675760476	35.0	33.0	40.0	9.0	41.0
41	31.4129677638194	35.0	31.0	40.0	7.0	41.0
42	30.724361575829526	35.0	28.0	40.0	7.0	41.0
43	29.86125328600676	35.0	23.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	16.0
11	14.0
12	11.0
13	7.0
14	6.0
15	4.0
16	9.0
17	16.0
18	38.0
19	99.0
20	207.0
21	461.0
22	892.0
23	1371.0
24	2341.0
25	3657.0
26	5861.0
27	8453.0
28	11768.0
29	15818.0
30	19325.0
31	22423.0
32	26603.0
33	32510.0
34	39839.0
35	47033.0
36	63482.0
37	117638.0
38	118675.0
39	121795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.95801179933856	19.926678134880735	13.573327922274583	13.541982143506123
2	16.019055810629094	27.00840127442548	34.30167056343659	22.670872351508837
3	24.552527650914023	33.097962372951166	28.720153367172642	13.629356608962167
4	13.068766884320448	17.506541727742984	30.248373653797227	39.17631773413934
5	9.349976377094261	47.662089476298355	29.230014416029654	13.757919730577731
6	28.708039056537487	30.353919585206	20.33235611227543	20.60568524598108
7	22.30199159266842	38.01849249518456	24.188492616327668	15.49102329581935
8	33.64310635153306	28.956685282324006	21.094497680109512	16.30571068603341
9	29.49880068324712	10.704053448338522	25.57618689958448	34.220958968829876
10	19.885186620955334	33.66309496408107	27.50569372599852	18.946024688965075
11	24.423964529298463	27.784474299489986	20.673373956655	27.118187214556556
12	19.453008589046238	35.50416732285849	30.57803433195634	14.464789756138927
13	28.63217318618484	22.264588658582383	33.134305304856625	15.968932850376149
14	22.897712818152083	24.967745647933903	35.40649569336257	16.72804584055144
15	25.734278653373227	25.30634063018644	34.30984772311532	14.649532993325016
16	16.800428846596485	27.272644675154762	37.18139363029547	18.745532847953285
17	15.03461664263995	31.550359189310335	35.64681333058742	17.768210837462295
18	13.378438949931553	25.46503809950695	41.23605339988128	19.92046955068022
19	14.886367766242262	29.048299756502356	43.71282420923837	12.352508268017008
20	16.286176360134228	25.375240771923874	45.06553842053618	13.273044447405722
21	19.01189625304372	26.582886113365717	41.203950476698125	13.201267156892438
22	17.948259779277258	27.034295613408123	40.64018074551468	14.377263861799944
23	15.863386918967375	28.756799156843982	40.91441845251796	14.465395471670684
24	13.453699104752445	31.14604407186209	41.349473633202905	14.050783190182562
25	13.756556870631275	30.128593407392152	41.61189988733691	14.502949834639658
26	14.48750408857984	30.47960555804572	41.25255914812168	13.780331205252766
27	13.429773341248016	32.230426302591255	40.696815147734014	13.642985208426714
28	13.122524137763941	31.793553975311035	42.57105043187517	12.51287145504985
29	13.158109925254704	32.65942432795862	42.32724993034272	11.855215816443964
30	12.39581692853768	33.675209274716224	41.52861400172023	12.400359795025864
31	11.914878796322096	34.34452493730844	41.022235817170824	12.718360449198638
32	10.956485396198529	35.07456358195937	41.12747889081372	12.841472131028384
33	11.057791318884998	35.19055810629096	41.0613044689692	12.690346105854847
34	11.878233006650756	35.09576362557089	39.82973336402292	13.196270003755437
35	11.17908585411947	35.71268489466607	39.166020570099455	13.942208681115002
36	11.434697808521205	35.52627593976765	37.96897525046337	15.070051001247775
37	11.410620616133839	35.33850412492277	38.473233430651625	14.77764182829176
38	11.577949531781893	34.65586272063188	38.45051909821072	15.315668649375509
39	11.541455170993494	35.183895235441625	38.88451427671508	14.390135316849795
40	11.351866209553345	35.13649799508159	38.61254800295589	14.899087792409174
41	10.97950258640532	35.51734163567422	37.79256060183896	15.710595176081505
42	10.928471052854738	35.516130204610704	37.4827371073449	16.07266163518965
43	10.884859534568186	35.396047100439745	36.527978000411885	17.191115364580178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	546.0
1	512.5
2	479.0
3	2080.5
4	3682.0
5	3682.0
6	5702.0
7	7722.0
8	8039.0
9	8356.0
10	12024.5
11	15693.0
12	15693.0
13	27102.5
14	38512.0
15	50569.5
16	62627.0
17	55370.0
18	48113.0
19	48113.0
20	48483.0
21	48853.0
22	35166.0
23	21479.0
24	20074.0
25	18669.0
26	18669.0
27	18819.0
28	18969.0
29	18838.5
30	18708.0
31	18843.5
32	18979.0
33	18979.0
34	19317.0
35	19655.0
36	20087.5
37	20520.0
38	23801.0
39	27082.0
40	27082.0
41	29606.5
42	32131.0
43	34467.5
44	36804.0
45	40279.5
46	43755.0
47	43755.0
48	42926.0
49	42097.0
50	36137.0
51	30177.0
52	25171.0
53	20165.0
54	20165.0
55	17429.0
56	14693.0
57	12934.5
58	11176.0
59	10053.5
60	8931.0
61	8931.0
62	7856.5
63	6782.0
64	5752.0
65	4722.0
66	4132.0
67	3542.0
68	3542.0
69	2920.0
70	2298.0
71	1992.5
72	1687.0
73	1429.5
74	1172.0
75	1172.0
76	968.5
77	765.0
78	591.0
79	417.0
80	322.0
81	227.0
82	227.0
83	174.5
84	122.0
85	85.5
86	49.0
87	32.5
88	16.0
89	16.0
90	8.5
91	1.0
92	2.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	660376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.13154811486106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85080430004112	25.793176684474446
2	10.124630313454434	6.708893527507927
3	3.655912157994675	3.6337808869891832
4	1.9066897783715013	2.5268633652891666
5	1.179394529127978	1.9537583294103753
6	0.8272480684652481	1.6444805509969345
7	0.6020256135907771	1.396222840814311
8	0.4529349590417472	1.2005149110715416
9	0.380936974678205	1.13589285347525
>10	2.5777424731635765	16.632066415490648
>50	0.25062179994106065	5.801967520758371
>100	0.16631757473165507	10.532071598242837
>500	0.01511977952105955	3.4397996917914506
>1k	0.007788977329030678	5.443566502543753
>5k	0.0	0.0
>10k+	0.0018327005480072182	12.156944321143794
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23808	3.605218845021624	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22832	3.4574242552727537	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18928	2.8662458962772726	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14517	2.198293093631507	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4334	0.6562927786594304	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3681	0.5574097180999915	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3400	0.5148582019940156	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2805	0.42475801664506285	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2703	0.4093122705852424	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	2485	0.37630077410444956	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	2002	0.3031606236447115	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1896	0.2871091620531334	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1858	0.28135486450143554	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1651	0.25000908573297637	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1605	0.2430433571177632	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1504	0.22774903994088216	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1332	0.20170327207530256	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1278	0.19352611239657405	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	1138	0.1723260687850558	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1107	0.16763177341393387	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	1081	0.16369462245750904	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	987	0.14946030746120392	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	968	0.14658315868535501	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	952	0.14416029655832435	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	940	0.14234314996305136	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	919	0.1391631434213236	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	893	0.13522599246489878	No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC	860	0.13022883932789805	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	807	0.12220310853210897	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	772	0.11690309762922942	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	753	0.11402594885338051	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	741	0.1122088022581075	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	732	0.11084594231165275	No Hit
GGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTC	699	0.10584878917465203	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	696	0.10539450252583377	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	685	0.10372878481350019	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	678	0.10266878263292427	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.571444146970816E-4	0.0
3	0.0	0.0	0.0	9.08573297636498E-4	0.0
4	0.0	0.0	0.0	0.002271433244091245	0.0
5	0.0	0.0	0.0	0.002422862127030661	0.0
6	0.0	0.0	0.0	0.002422862127030661	0.0
7	0.0	0.0	0.0	0.0028771487758489102	0.0
8	0.0	0.0	0.0	0.0030285776587883266	0.0
9	0.0	0.0	0.0	0.005451439785818988	0.0
10	0.0	0.0	0.0	0.009691448508122645	0.0
11	0.0	0.0	0.0	0.016657177123335795	0.0
12	0.0	0.0	0.0	0.027862914460852605	0.0
13	0.0	0.0	0.0	0.03316292536373218	0.0
14	0.0	0.0	0.0	0.04800295589179498	0.0
15	0.0	0.0	0.0	0.0704144305668286	0.0
16	0.0	0.0	0.0	0.12129453523447248	0.0
17	0.0	0.0	0.0	0.18307751947375434	0.0
18	0.0	0.0	0.0	0.20806328515875805	0.0
19	0.0	0.0	0.0	0.2547033811040983	0.0
20	0.0	0.0	0.0	0.2890777375313458	0.0
21	0.0	0.0	0.0	0.3643378923522357	0.0
22	0.0	0.0	0.0	0.5047124668370746	0.0
23	0.0	0.0	0.0	0.7321586490120779	0.0
24	0.0	0.0	0.0	1.0698450579669765	0.0
25	0.0	0.0	0.0	1.1479823615637152	0.0
26	0.0	0.0	0.0	1.2682168946176118	0.0
27	0.0	0.0	0.0	1.3533199268295637	0.0
28	0.0	0.0	0.0	1.464771584672974	0.0
29	0.0	0.0	0.0	1.6140804632512387	0.0
30	0.0	0.0	0.0	1.8813524416393086	0.0
31	0.0	0.0	0.0	2.6166910971931143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATAGT	30	3.5978353E-4	30.833332	1
TAGTTCC	25	0.00549467	29.6	4
ATTGAGT	45	4.0048617E-6	28.777777	1
TGGCCAC	35	8.865384E-4	26.42857	22
GTTATAC	35	8.865384E-4	26.42857	3
TGAGTAT	45	1.3223794E-4	24.666666	3
TTGAGTA	45	1.3223794E-4	24.666666	2
GTATAGA	45	1.3223794E-4	24.666666	1
TTGGTAA	75	1.371518E-8	24.666664	33
GTAAACA	75	1.371518E-8	24.666664	36
GCAGCGT	85	1.9736035E-9	23.941177	1
ATACACT	85	1.9736035E-9	23.941177	4
TACACTG	95	2.8012437E-10	23.368422	5
TCCTGTG	40	0.0019303213	23.125002	7
ACAGGTA	40	0.0019303213	23.125002	24
ATTGGCC	40	0.0019303213	23.125002	20
CTAGAGG	90	3.8216967E-9	22.61111	18
TGCTAGA	90	3.8216967E-9	22.61111	16
CTCTAAT	90	3.8216967E-9	22.61111	1
AATACTG	90	3.8216967E-9	22.61111	5
>>END_MODULE