FastQCFastQC Report
Fri 10 Feb 2017
ERR1630790.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630790.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220403
Sequences flagged as poor quality0
Sequence length43
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85793.8924152575055695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82943.7631066727766864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58152.6383488428015953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43751.9850002041714494No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.5848377744404567No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.5744023447956698No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT10620.4818446209897324No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.4351120447543817No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT9560.4337509017572356TruSeq Adapter, Index 11 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8900.4038057558200206No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA8220.3729531812180415No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7040.3194148899969601No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6790.308072031687409No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.2790343144149581No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6120.2776731714178119No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC5760.2613394554520583No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG5720.2595245981225301No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5590.2536263118015635No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA5450.24727431114821485No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.2418297391596303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5090.2309405951824612No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC4720.21415316488432556No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.2028103065747744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4170.18919887660331303No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.17830973262614394No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA3680.1669668743165928No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3490.15834630200133393No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.15380915867751346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3350.15199430134798528No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA3200.1451885863622546No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3140.14246630036796232No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA2740.12431772707268049No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC2640.11978058374886005No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2560.11615086908980368No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2350.10662286810978072No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA2350.10662286810978072No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.1052617251126346No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2310.10480801078025254No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2210.10027086745643207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCTT352.3796221E-531.71428710
TTTAGGT250.0054868329.59999834
CTCCGGT250.0054868329.5999987
GAATGGA250.0054868329.5999989
TTACTCC250.0054868329.5999984
CGGTCTG250.0054868329.59999810
ACTCAGA250.0054868329.59999818
CTCAGAT250.0054868329.59999819
GCCGTCT1050.026.42857436
CCAGTGC358.846572E-426.4285728
CAGCGTC659.319956E-825.6153852
AGCGTCA659.319956E-825.6153853
CCGTCTT1100.025.22727237
GCAGCGT751.3609679E-824.6666661
GTGCTTC502.692513E-422.19999911
TGGGCAG1650.021.303036
ACCATTC705.071115E-621.1428599
GGGCAGT1600.020.81257
CAGTCGG1351.8189894E-1220.55555310
ATGCCGT1351.8189894E-1220.55555334
Produced by FastQC (version 0.11.5)