##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630790.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	220403
Sequences flagged as poor quality	0
Sequence length	43
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.170850669001783	31.0	31.0	33.0	28.0	34.0
2	31.18248390448406	31.0	31.0	34.0	28.0	34.0
3	31.092285495206507	31.0	31.0	34.0	27.0	34.0
4	34.97170637423265	35.0	35.0	37.0	32.0	37.0
5	34.88414404522624	35.0	35.0	37.0	32.0	37.0
6	35.065561721029205	36.0	35.0	37.0	32.0	37.0
7	34.92024155751056	36.0	35.0	37.0	32.0	37.0
8	35.07659151644941	36.0	35.0	37.0	32.0	37.0
9	36.37574806150551	38.0	35.0	39.0	32.0	39.0
10	36.247097362558584	38.0	35.0	39.0	32.0	39.0
11	36.579452185315084	38.0	35.0	39.0	32.0	39.0
12	36.508718120896724	38.0	35.0	39.0	32.0	39.0
13	36.63426995095348	38.0	35.0	39.0	32.0	39.0
14	37.40506254452072	39.0	36.0	40.0	32.0	41.0
15	37.35140628757322	39.0	36.0	40.0	32.0	41.0
16	37.13322867656066	39.0	36.0	40.0	32.0	41.0
17	37.412394568132015	39.0	36.0	40.0	32.0	41.0
18	37.52318707095638	39.0	37.0	40.0	32.0	41.0
19	37.71674160515057	39.0	37.0	40.0	33.0	41.0
20	37.726142566117524	39.0	37.0	40.0	33.0	41.0
21	37.704659192479234	39.0	37.0	40.0	33.0	41.0
22	37.56133083487974	39.0	36.0	40.0	32.0	41.0
23	37.405548018856365	39.0	36.0	40.0	32.0	41.0
24	37.21989718833228	39.0	36.0	40.0	32.0	41.0
25	37.09905491304565	39.0	35.0	40.0	32.0	41.0
26	36.64365276334714	38.0	35.0	40.0	31.0	41.0
27	36.26065888395348	38.0	35.0	40.0	31.0	41.0
28	35.962545881861864	38.0	35.0	40.0	30.0	41.0
29	35.65192397562647	37.0	35.0	40.0	30.0	41.0
30	35.241448619120426	37.0	34.0	40.0	29.0	41.0
31	34.784335966388845	36.0	34.0	39.0	27.0	41.0
32	34.32362082185814	35.0	33.0	39.0	25.0	41.0
33	33.89723824085879	35.0	33.0	39.0	24.0	41.0
34	33.512538395575376	35.0	33.0	39.0	21.0	40.0
35	33.080239379681764	35.0	33.0	39.0	21.0	40.0
36	32.60971946842829	35.0	33.0	39.0	18.0	40.0
37	31.978661814948072	35.0	31.0	38.0	15.0	40.0
38	31.322327736010852	35.0	31.0	38.0	10.0	40.0
39	30.713905890573177	35.0	29.0	38.0	8.0	40.0
40	29.93383937605205	35.0	25.0	38.0	7.0	40.0
41	29.258367626574955	35.0	22.0	38.0	7.0	40.0
42	28.498260005535315	35.0	20.0	38.0	7.0	40.0
43	27.61239184584602	33.0	17.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	0.0
13	2.0
14	2.0
15	5.0
16	7.0
17	19.0
18	44.0
19	106.0
20	233.0
21	398.0
22	701.0
23	1066.0
24	1631.0
25	2374.0
26	3426.0
27	4756.0
28	6087.0
29	7703.0
30	8988.0
31	10475.0
32	12442.0
33	14708.0
34	17527.0
35	21924.0
36	30849.0
37	37668.0
38	28110.0
39	9148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.95892524148945	21.132198745026155	14.660417507928658	13.248458505555732
2	16.04878336501772	27.683833704622895	32.308090180260706	23.959292750098683
3	25.674786640835194	31.14703520369505	30.130715099159268	13.047463056310486
4	13.822407136019018	18.378152747467137	28.1638634682831	39.63557664823074
5	9.329727816772005	48.12366437843405	28.550881793804983	13.995726010988962
6	28.962400693275498	29.14660871222261	20.019237487692997	21.871753106808892
7	21.547347359155726	39.26579946733938	22.850868636089345	16.335984537415552
8	35.01948703057581	28.695162951502475	21.036918735225925	15.24843128269579
9	28.370757203849312	10.694500528577198	25.871244946756622	35.063497320816865
10	20.546453541920936	34.5117806926403	26.25780955794613	18.68395620749264
11	24.45474880105988	26.565881589633534	20.371319809621465	28.60804979968512
12	18.307827025947923	36.656034627477844	30.011388229742792	15.02475011683144
13	29.271380153627675	20.830932428324477	34.12793836744509	15.76974905060276
14	21.906689110402308	25.525968339813886	36.22818201204158	16.339160537742224
15	26.476953580486658	25.221979737117916	33.532211449027464	14.768855233367967
16	17.204847483927168	25.88803237705476	38.04077076990785	18.86634936911022
17	14.300168328017312	33.3947360063157	35.9042299787208	16.400865686946183
18	12.88866303997677	24.85492484222084	41.48582369568472	20.770588422117665
19	14.29744604202302	29.588980186295107	44.03342967200991	12.080144099671966
20	15.810583340517143	25.08132829407948	45.68857955653961	13.419508808863764
21	18.689400779481222	25.613535205963622	42.691342676823815	13.005721337731337
22	17.101400616144062	28.034101169221838	41.55388084554203	13.310617369092073
23	15.168123845864168	28.266402907401446	41.92592659809531	14.639546648639085
24	13.012527052717068	31.1710820633113	42.790706115615485	13.025684768356147
25	12.691751019722963	30.995494616679448	42.221748342808404	14.091006020789191
26	14.100987736101594	30.338516263390243	42.47038379695376	13.0901122035544
27	12.39411441768034	32.8970113836926	41.643262569021296	13.065611629605767
28	12.322427553163976	31.642944968988623	43.690875351061464	12.343752126785933
29	12.0084572351556	32.85935309410489	42.95086727494635	12.181322395793162
30	11.718987491095856	33.70371546666788	42.87963412476237	11.6976629174739
31	11.290681161327205	33.95008234915133	42.17093233758161	12.588304151939855
32	10.30249134539911	35.669205954546904	41.93545459907533	12.092848100978662
33	10.661379382313307	34.630653847724396	42.199062626189296	12.508904143772998
34	11.199484580518414	35.453237932333046	40.31161100347999	13.035666483668553
35	10.821086827311788	35.78898653829576	40.29482357318185	13.095103061210601
36	11.890037794403888	35.19462076287528	38.1759776409577	14.739363801763133
37	11.15411314728021	35.51948022486082	38.682322835896066	14.644083791962903
38	11.699024060471046	34.20824580427671	38.67097997758651	15.42175015766573
39	12.218526971048489	34.59707898712813	38.44729881172216	14.737095230101223
40	11.729422920740644	34.51904012195841	38.928689718379516	14.82284723892143
41	11.626429767289919	35.324383061936544	36.82617750212111	16.223009668652423
42	11.836499503182806	34.65243213567874	37.23270554393543	16.278362817203032
43	11.410915459408447	35.09026646642741	36.044881421759236	17.453936652404913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	89.5
2	114.0
3	429.0
4	744.0
5	744.0
6	1246.5
7	1749.0
8	2108.0
9	2467.0
10	3529.5
11	4592.0
12	4592.0
13	8626.0
14	12660.0
15	17640.0
16	22620.0
17	20323.5
18	18027.0
19	18027.0
20	18943.5
21	19860.0
22	14652.0
23	9444.0
24	8234.5
25	7025.0
26	7025.0
27	7131.5
28	7238.0
29	7151.0
30	7064.0
31	6865.0
32	6666.0
33	6666.0
34	6346.0
35	6026.0
36	5863.5
37	5701.0
38	6305.5
39	6910.0
40	6910.0
41	7430.0
42	7950.0
43	8627.5
44	9305.0
45	10364.5
46	11424.0
47	11424.0
48	12124.0
49	12824.0
50	11541.0
51	10258.0
52	7784.5
53	5311.0
54	5311.0
55	5542.0
56	5773.0
57	5106.5
58	4440.0
59	3759.0
60	3078.0
61	3078.0
62	2834.5
63	2591.0
64	2400.0
65	2209.0
66	2041.5
67	1874.0
68	1874.0
69	1674.0
70	1474.0
71	1328.0
72	1182.0
73	964.5
74	747.0
75	747.0
76	597.5
77	448.0
78	364.5
79	281.0
80	215.0
81	149.0
82	149.0
83	109.5
84	70.0
85	50.0
86	30.0
87	18.5
88	7.0
89	7.0
90	5.5
91	4.0
92	2.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	220403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.50801486368153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.66817537743253	36.793963784521985
2	8.698533084599937	7.743088796431991
3	2.9674709726087443	3.9622872646924048
4	1.3690530801145806	2.437353393556349
5	0.8746444845408116	1.9464344859189755
6	0.6146976971772837	1.641538454558241
7	0.4495550322639836	1.4006161440633749
8	0.3414987206540465	1.2159544107838822
9	0.24159760237316125	0.9677726709709032
>10	1.5311375475294862	12.542025290036888
>50	0.13456068992935563	4.063919275145982
>100	0.08766832828730746	7.040739009904584
>500	0.014271588325840748	4.324351301933277
>1k	0.004077596664525928	3.6260849443973084
>5k	0.0030581974983944463	10.293870773083851
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8579	3.8924152575055695	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8294	3.7631066727766864	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5815	2.6383488428015953	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4375	1.9850002041714494	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1289	0.5848377744404567	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1266	0.5744023447956698	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	1062	0.4818446209897324	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	959	0.4351120447543817	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	956	0.4337509017572356	TruSeq Adapter, Index 11 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	890	0.4038057558200206	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	822	0.3729531812180415	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	704	0.3194148899969601	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	679	0.308072031687409	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	615	0.2790343144149581	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	612	0.2776731714178119	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	576	0.2613394554520583	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	572	0.2595245981225301	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	559	0.2536263118015635	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	545	0.24727431114821485	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	533	0.2418297391596303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	509	0.2309405951824612	No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	472	0.21415316488432556	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	447	0.2028103065747744	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	417	0.18919887660331303	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	393	0.17830973262614394	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	368	0.1669668743165928	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	349	0.15834630200133393	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	339	0.15380915867751346	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	335	0.15199430134798528	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	320	0.1451885863622546	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	314	0.14246630036796232	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	274	0.12431772707268049	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	264	0.11978058374886005	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	256	0.11615086908980368	No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	235	0.10662286810978072	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	235	0.10662286810978072	No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	232	0.1052617251126346	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	231	0.10480801078025254	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	221	0.10027086745643207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.5371433238204563E-4	0.0
3	0.0	0.0	0.0	0.002268571661910228	0.0
4	0.0	0.0	0.0	0.002268571661910228	0.0
5	0.0	0.0	0.0	0.0027222859942922736	0.0
6	0.0	0.0	0.0	0.0027222859942922736	0.0
7	0.0	0.0	0.0	0.003176000326674319	0.0
8	0.0	0.0	0.0	0.003176000326674319	0.0
9	0.0	0.0	0.0	0.003629714659056365	0.0
10	0.0	0.0	0.0	0.003629714659056365	0.0
11	0.0	0.0	0.0	0.004990857656202502	0.0
12	0.0	0.0	0.0	0.009981715312405003	0.0
13	0.0	0.0	0.0	0.01134285830955114	0.0
14	0.0	0.0	0.0	0.016333715965753642	0.0
15	0.0	0.0	0.0	0.028130288607686828	0.0
16	0.0	0.0	0.0	0.04900114789726093	0.0
17	0.0	0.0	0.0	0.08802058048211685	0.0
18	0.0	0.0	0.0	0.099363438791668	0.0
19	0.0	0.0	0.0	0.12567887006982664	0.0
20	0.0	0.0	0.0	0.14700344369178278	0.0
21	0.0	0.0	0.0	0.18330059028234644	0.0
22	0.0	0.0	0.0	0.25680231212823784	0.0
23	0.0	0.0	0.0	0.3502674645989392	0.0
24	0.0	0.0	0.0	0.5394663412022522	0.0
25	0.0	0.0	0.0	0.5757634877928159	0.0
26	0.0	0.0	0.0	0.6483577809739431	0.0
27	0.0	0.0	0.0	0.7028035008597887	0.0
28	0.0	0.0	0.0	0.7772126513704442	0.0
29	0.0	0.0	0.0	0.8774835188268763	0.0
30	0.0	0.0	0.0	1.1088778283417196	0.0
31	0.0	0.0	0.0	1.6937156027821763	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGCTT	35	2.3796221E-5	31.714287	10
TTTAGGT	25	0.00548683	29.599998	34
CTCCGGT	25	0.00548683	29.599998	7
GAATGGA	25	0.00548683	29.599998	9
TTACTCC	25	0.00548683	29.599998	4
CGGTCTG	25	0.00548683	29.599998	10
ACTCAGA	25	0.00548683	29.599998	18
CTCAGAT	25	0.00548683	29.599998	19
GCCGTCT	105	0.0	26.428574	36
CCAGTGC	35	8.846572E-4	26.428572	8
CAGCGTC	65	9.319956E-8	25.615385	2
AGCGTCA	65	9.319956E-8	25.615385	3
CCGTCTT	110	0.0	25.227272	37
GCAGCGT	75	1.3609679E-8	24.666666	1
GTGCTTC	50	2.692513E-4	22.199999	11
TGGGCAG	165	0.0	21.30303	6
ACCATTC	70	5.071115E-6	21.142859	9
GGGCAGT	160	0.0	20.8125	7
CAGTCGG	135	1.8189894E-12	20.555553	10
ATGCCGT	135	1.8189894E-12	20.555553	34
>>END_MODULE