FastQCFastQC Report
Fri 10 Feb 2017
ERR1630789.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630789.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479624
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT133570.53867037905747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116510.4698696253948179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94930.38284030159411264No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65550.26435459569676695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG450.003827037520.5555575
ACGTTAA1001.2916644E-820.3520
CGCAAGA3950.018.2658232
AAGACGG4200.017.6190475
CGAATTA1054.802805E-717.61904715
CTTATAC18450.016.24390237
CAGTCGG3550.016.1549310
GCGCAAG4250.016.1058831
GGTATCA57950.015.7704931
CAAGACG4600.015.6847834
ATACCGT2950.015.6779656
GCGAAAG4250.015.670588518
TACACCG957.064946E-515.5789485
GACGGAC4300.015.4883727
TACGTTA1201.937602E-615.41666619
AGTCGGT3400.015.23529411
AATTACG1101.4531368E-515.13636416
CTAGGAC2450.015.102043
CGGACCA4600.014.8804359
ACGGACC4750.014.88
Produced by FastQC (version 0.11.5)