##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630789.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2479624
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95986004329689	33.0	31.0	34.0	30.0	34.0
2	32.14442754224027	33.0	31.0	34.0	30.0	34.0
3	32.24096919532961	34.0	31.0	34.0	30.0	34.0
4	35.85498043251719	37.0	35.0	37.0	35.0	37.0
5	35.81094069100799	37.0	35.0	37.0	35.0	37.0
6	35.88596254916068	37.0	35.0	37.0	35.0	37.0
7	35.83687809119447	37.0	35.0	37.0	35.0	37.0
8	35.84255032214561	37.0	35.0	37.0	35.0	37.0
9	37.53071191438702	39.0	37.0	39.0	35.0	39.0
10	37.432016305697964	39.0	37.0	39.0	35.0	39.0
11	37.52718678315745	39.0	37.0	39.0	35.0	39.0
12	37.47716871590209	39.0	37.0	39.0	35.0	39.0
13	37.5168606208038	39.0	37.0	39.0	35.0	39.0
14	38.78797914522524	40.0	38.0	41.0	35.0	41.0
15	38.79786169193394	40.0	38.0	41.0	35.0	41.0
16	38.76067339241756	40.0	38.0	41.0	35.0	41.0
17	38.755471797337016	40.0	38.0	41.0	35.0	41.0
18	38.749307556306924	40.0	38.0	41.0	35.0	41.0
19	38.82089502279378	40.0	38.0	41.0	35.0	41.0
20	38.784986352769614	40.0	38.0	41.0	35.0	41.0
21	38.74679346546089	40.0	38.0	41.0	35.0	41.0
22	38.677125644855835	40.0	38.0	41.0	34.0	41.0
23	38.621111103941566	40.0	38.0	41.0	34.0	41.0
24	38.57036510374154	40.0	38.0	41.0	34.0	41.0
25	38.51733649940475	40.0	38.0	41.0	34.0	41.0
26	38.38277053295177	40.0	38.0	41.0	34.0	41.0
27	38.25665504124819	40.0	38.0	41.0	34.0	41.0
28	38.16757137372441	40.0	38.0	41.0	34.0	41.0
29	38.09222930573345	40.0	38.0	41.0	33.0	41.0
30	37.99695155394527	40.0	37.0	41.0	33.0	41.0
31	37.93780710301239	40.0	37.0	41.0	33.0	41.0
32	37.829164018415696	40.0	37.0	41.0	33.0	41.0
33	37.753819933989995	40.0	37.0	41.0	33.0	41.0
34	37.68359194781144	40.0	37.0	41.0	33.0	41.0
35	37.621051820759924	40.0	37.0	41.0	33.0	41.0
36	37.57620429548996	40.0	37.0	41.0	33.0	41.0
37	37.439347255874274	40.0	37.0	41.0	32.0	41.0
38	37.2883461363497	40.0	36.0	41.0	32.0	41.0
39	37.22457921039641	40.0	36.0	41.0	32.0	41.0
40	37.03986773801189	40.0	36.0	41.0	31.0	41.0
41	36.97221957845222	40.0	36.0	41.0	31.0	41.0
42	36.829928650472816	39.0	36.0	41.0	31.0	41.0
43	36.05730949531058	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	5.0
11	10.0
12	7.0
13	7.0
14	8.0
15	7.0
16	3.0
17	45.0
18	94.0
19	166.0
20	323.0
21	620.0
22	1102.0
23	1883.0
24	3355.0
25	5390.0
26	8423.0
27	12536.0
28	18439.0
29	26061.0
30	35297.0
31	46147.0
32	59814.0
33	77703.0
34	103767.0
35	139821.0
36	197853.0
37	316086.0
38	598845.0
39	825797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53418098873055	19.759407071394694	12.548475091384823	24.157936848489932
2	17.816814162147164	21.721599726410133	36.19262436562963	24.268961745813076
3	19.872690375637596	24.502303575058153	30.29015689475501	25.33484915454924
4	14.059147677228484	15.910476749700761	35.07422899600907	34.95614657706168
5	13.38473091081551	38.49426364642382	34.623555829432206	13.497449613328472
6	33.65857081557527	35.462231370562634	16.388371785399723	14.490826028462381
7	27.921773623742958	31.616245043603385	22.15525418369882	18.30672714895484
8	26.70832352001755	34.405417918200506	20.680595122486313	18.205663439295634
9	26.460705332743995	13.864239094314298	20.72463405742161	38.9504215155201
10	16.169507957658098	27.92677438192242	33.915827560952785	21.987890099466693
11	34.00289721344849	22.39214493810352	22.560355924930555	21.044601923517437
12	20.838441634699453	26.688683445554652	30.38444538365494	22.088429536090956
13	29.390867325046056	20.62611105554713	26.606977509493372	23.376044109913437
14	22.308462895987454	21.77939074633896	26.98239733120828	28.929749026465302
15	24.891435153071594	28.41019444883579	24.04045935996748	22.657911038125135
16	23.84982561872284	27.494410442873594	25.485436501663155	23.170327436740408
17	22.396379451078065	27.620598929515122	27.059505796040046	22.92351582336677
18	21.847667226966667	26.135615722383715	28.87574890386607	23.140968146783543
19	22.85007726977961	26.493169932215526	28.839291763589962	21.817461034414894
20	23.46863879362355	25.596017783341345	28.87764435253087	22.05769907050424
21	23.604062551419087	26.221233541859572	27.596321055127714	22.578382851593627
22	23.43250428290741	26.10327210899717	27.72863950340858	22.73558410468684
23	22.751876897465102	26.38718612176685	27.995534806890078	22.865402173877975
24	23.015949192296898	26.666341348527034	27.90616641877962	22.41154304039645
25	23.01481998883702	26.202480698686575	28.01198891444832	22.770710398028086
26	22.798456540185125	26.636740086400195	28.00944820666359	22.555355166751088
27	23.06470658454669	26.309351740425164	27.601200827222193	23.02474084780596
28	22.420012066345542	26.54176600968534	28.293604191603244	22.744617732365874
29	22.18614596406552	26.990100111952458	28.41140430968566	22.41234961429636
30	22.054835733159546	27.6160821156756	28.18870925591945	22.14037289524541
31	22.609597261520296	27.046479627556437	27.87862192009756	22.465301190825706
32	21.678488351459738	27.125927156697948	28.085266153255496	23.11031833858682
33	22.010958113004232	26.709775353037397	28.49678015699154	22.782486376966833
34	22.54353885911735	26.33221811048772	28.33554603439876	22.788696995996165
35	22.382062764354597	26.559712278958425	28.519162582714152	22.53906237397283
36	22.050883521049965	26.769623136411003	28.184958687284844	22.994534655254185
37	22.564550109210106	25.984786403099825	28.219076763251206	23.231586724438866
38	22.0636677173636	26.09794872125774	28.870788474381598	22.96759508699706
39	22.080968727516755	25.92768097098592	28.936604904614573	23.054745396882755
40	21.529352837365664	25.78354621507132	29.634936587159988	23.05216436040303
41	20.998264252967385	25.96171838956229	29.923004455514224	23.1170129019561
42	21.43502401977074	25.70349375550487	29.691074130593993	23.1704080941304
43	20.867195994231384	25.568876571609245	29.63465428629502	23.929273147864354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	547.0
1	504.5
2	462.0
3	1480.5
4	2499.0
5	2499.0
6	3460.0
7	4421.0
8	4422.0
9	4423.0
10	6379.5
11	8336.0
12	8336.0
13	13351.5
14	18367.0
15	26179.0
16	33991.0
17	32602.5
18	31214.0
19	31214.0
20	34696.0
21	38178.0
22	36910.5
23	35643.0
24	39885.5
25	44128.0
26	44128.0
27	49885.5
28	55643.0
29	66602.5
30	77562.0
31	84844.0
32	92126.0
33	92126.0
34	99029.5
35	105933.0
36	112627.5
37	119322.0
38	128972.0
39	138622.0
40	138622.0
41	145578.0
42	152534.0
43	149454.5
44	146375.0
45	151586.0
46	156797.0
47	156797.0
48	158717.5
49	160638.0
50	163016.0
51	165394.0
52	171994.5
53	178595.0
54	178595.0
55	165398.5
56	152202.0
57	144081.0
58	135960.0
59	123734.0
60	111508.0
61	111508.0
62	102412.5
63	93317.0
64	81643.5
65	69970.0
66	60359.5
67	50749.0
68	50749.0
69	42818.0
70	34887.0
71	29931.0
72	24975.0
73	19684.5
74	14394.0
75	14394.0
76	11321.0
77	8248.0
78	6781.0
79	5314.0
80	4072.0
81	2830.0
82	2830.0
83	2138.5
84	1447.0
85	1212.0
86	977.0
87	844.0
88	711.0
89	711.0
90	521.0
91	331.0
92	186.0
93	41.0
94	26.0
95	11.0
96	11.0
97	6.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2479624.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.032135391439844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.4288140090911	48.99584365514267
2	9.838669049342116	11.419179486859807
3	2.5178722711935215	4.383525136207638
4	1.002705653258587	2.327566009106578
5	0.5138263063883424	1.4909218890005864
6	0.31542243368925293	1.0982782426411308
7	0.20627354933060957	0.8379346179698747
8	0.15051389423284492	0.6987714150730651
9	0.11076459054556902	0.5785115143606073
>10	0.7248291637602187	8.010917587614928
>50	0.0886278250581442	3.6449568911967343
>100	0.09230478523104119	10.765617592595717
>500	0.007709541078043646	3.034629171221814
>1k	0.0013891065005482244	1.0585268936314645
>5k	1.3891065005482243E-4	0.6468372396998607
>10k+	1.3891065005482243E-4	1.0079826576778488
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13357	0.53867037905747	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11651	0.4698696253948179	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9493	0.38284030159411264	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6555	0.26435459569676695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.03286949956929E-5	0.0
2	0.0	0.0	0.0	2.823008649698503E-4	0.0
3	0.0	0.0	0.0	6.855878149267792E-4	0.0
4	0.0	0.0	0.0	0.001169532154875094	0.0
5	0.0	4.03286949956929E-5	0.0	0.0012905182398621727	0.0
6	0.0	4.03286949956929E-5	0.0	0.0013308469348578655	0.0
7	0.0	4.03286949956929E-5	0.0	0.0017744625798104875	0.0
8	0.0	4.03286949956929E-5	0.0	0.0020164347497846446	0.0
9	0.0	4.03286949956929E-5	0.0	0.003750568634599439	0.0
10	8.06573899913858E-5	4.03286949956929E-5	0.0	0.0062509477243323985	0.0
11	8.06573899913858E-5	4.03286949956929E-5	0.0	0.007299493794220414	0.0
12	8.06573899913858E-5	4.03286949956929E-5	0.0	0.008428697254099815	0.0
13	8.06573899913858E-5	4.03286949956929E-5	0.0	0.008952970289043823	0.0
14	8.06573899913858E-5	4.03286949956929E-5	0.0	0.010203159833910302	0.0
15	8.06573899913858E-5	4.03286949956929E-5	0.0	0.012017951108716483	0.0
16	8.06573899913858E-5	4.03286949956929E-5	0.0	0.015808848438311617	0.0
17	8.06573899913858E-5	4.03286949956929E-5	0.0	0.020406319667820604	0.0
18	8.06573899913858E-5	4.03286949956929E-5	0.0	0.022301768332618172	0.0
19	8.06573899913858E-5	4.03286949956929E-5	0.0	0.025165105677312366	0.0
20	8.06573899913858E-5	4.03286949956929E-5	0.0	0.02843172997196349	0.0
21	8.06573899913858E-5	4.03286949956929E-5	0.0	0.03492464986627005	0.0
22	8.06573899913858E-5	4.03286949956929E-5	0.0	0.04674095750000806	0.0
23	8.06573899913858E-5	4.03286949956929E-5	0.0	0.0687200962726607	0.0
24	8.06573899913858E-5	4.03286949956929E-5	0.0	0.1016283113891461	0.0
25	8.06573899913858E-5	4.03286949956929E-5	0.0	0.1078792591134785	0.0
26	1.2098608498707869E-4	4.03286949956929E-5	0.0	0.11953425196723375	0.0
27	1.2098608498707869E-4	4.03286949956929E-5	0.0	0.13546408649053243	0.0
28	1.2098608498707869E-4	8.06573899913858E-5	0.0	0.17155826851167758	0.0
29	1.2098608498707869E-4	8.06573899913858E-5	0.0	0.22507444677096206	0.0
30	1.2098608498707869E-4	8.06573899913858E-5	0.0	0.31653992702119355	0.0
31	1.2098608498707869E-4	8.06573899913858E-5	0.0	0.6524376276403197	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	45	0.0038270375	20.555557	5
ACGTTAA	100	1.2916644E-8	20.35	20
CGCAAGA	395	0.0	18.265823	2
AAGACGG	420	0.0	17.619047	5
CGAATTA	105	4.802805E-7	17.619047	15
CTTATAC	1845	0.0	16.243902	37
CAGTCGG	355	0.0	16.15493	10
GCGCAAG	425	0.0	16.105883	1
GGTATCA	5795	0.0	15.770493	1
CAAGACG	460	0.0	15.684783	4
ATACCGT	295	0.0	15.677965	6
GCGAAAG	425	0.0	15.6705885	18
TACACCG	95	7.064946E-5	15.578948	5
GACGGAC	430	0.0	15.488372	7
TACGTTA	120	1.937602E-6	15.416666	19
AGTCGGT	340	0.0	15.235294	11
AATTACG	110	1.4531368E-5	15.136364	16
CTAGGAC	245	0.0	15.10204	3
CGGACCA	460	0.0	14.880435	9
ACGGACC	475	0.0	14.8	8
>>END_MODULE