FastQCFastQC Report
Fri 10 Feb 2017
ERR1630788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715084
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT171682.4008368247646428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158672.218900157184331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130991.8318127660526595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82921.1595840488669862No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.3154874112691656No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.2936717924048084No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.2813655458659402No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT18700.2615077389509484No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT14340.20053588109928347TruSeq Adapter, Index 1 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.19899760028192492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11870.16599448456405122No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11490.16068042355863088No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11090.1550866751318726No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.13830542985159786No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9090.12711793299808133No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG8790.12292262167801266No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC8520.11914684148995085No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8370.11704918582991648No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC7950.11117574998182032No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC7950.11117574998182032No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.11047653142847554No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC7890.11033668771780658No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7650.10698043866175164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7260.10152653394566231No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA7240.10124684652432442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTA303.5981293E-430.83333413
CTAGTGT601.336226E-624.66666818
CCGTCTT2150.024.09302337
GTACTAT952.8012437E-1023.3684221
CCTAGTG652.680492E-622.7692317
GCCGTCT2300.022.5217436
CCATATG502.700719E-422.1999998
ATCGGAG502.700719E-422.19999918
AATCGGA502.700719E-422.19999917
GCTATAT806.9525413E-720.812519
GAGACTA450.003824415620.55555526
TGTAGTA555.140483E-420.1818182
AATACCC555.140483E-420.1818185
CGAGTTC656.8974674E-519.92307714
CCGAGTT656.8974674E-519.92307713
TCTATCG759.257743E-619.73333431
TAGTGTC851.2439614E-619.58823619
AGTCCTT801.6152786E-518.512
GACCGTG801.6152786E-518.57
TCTAGGC609.2321605E-418.52
Produced by FastQC (version 0.11.5)