##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630788.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	715084
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.608977406850105	31.0	31.0	34.0	30.0	34.0
2	31.704566176840764	31.0	31.0	34.0	30.0	34.0
3	31.729101755877632	31.0	31.0	34.0	30.0	34.0
4	35.45741898853841	37.0	35.0	37.0	33.0	37.0
5	35.4486801550587	37.0	35.0	37.0	33.0	37.0
6	35.576782587779896	37.0	35.0	37.0	33.0	37.0
7	35.50123202309099	37.0	35.0	37.0	33.0	37.0
8	35.565564325310035	37.0	35.0	37.0	33.0	37.0
9	37.086563256904086	39.0	37.0	39.0	33.0	39.0
10	36.98740567541715	39.0	37.0	39.0	33.0	39.0
11	37.15540971410352	39.0	37.0	39.0	34.0	39.0
12	37.10425068942949	39.0	37.0	39.0	33.0	39.0
13	37.16664755469287	39.0	37.0	39.0	33.0	39.0
14	38.19143345397184	40.0	38.0	41.0	33.0	41.0
15	38.15987632222228	40.0	37.0	41.0	33.0	41.0
16	38.06196754507163	40.0	37.0	41.0	33.0	41.0
17	38.18614876014566	40.0	38.0	41.0	33.0	41.0
18	38.24101923690084	40.0	38.0	41.0	33.0	41.0
19	38.36156451549748	40.0	38.0	41.0	34.0	41.0
20	38.350515463917525	40.0	38.0	41.0	34.0	41.0
21	38.29925854864603	40.0	38.0	41.0	34.0	41.0
22	38.1754744897103	40.0	38.0	41.0	34.0	41.0
23	38.045633799665495	40.0	37.0	41.0	33.0	41.0
24	37.93223173780982	40.0	37.0	41.0	33.0	41.0
25	37.841255013397024	40.0	37.0	41.0	33.0	41.0
26	37.56556572374714	39.0	36.0	41.0	33.0	41.0
27	37.31612789546403	39.0	35.0	41.0	32.0	41.0
28	37.124667871187164	39.0	35.0	41.0	32.0	41.0
29	36.95499969234384	39.0	35.0	40.0	31.0	41.0
30	36.69902556902406	39.0	35.0	40.0	31.0	41.0
31	36.45037366239491	38.0	35.0	40.0	31.0	41.0
32	36.157273830766734	38.0	35.0	40.0	30.0	41.0
33	35.8891416952414	38.0	35.0	40.0	30.0	41.0
34	35.66398772731595	38.0	35.0	40.0	29.0	41.0
35	35.41726426545693	38.0	35.0	40.0	28.0	41.0
36	35.1996185063573	38.0	35.0	40.0	26.0	41.0
37	34.8459747386321	38.0	34.0	40.0	24.0	41.0
38	34.46819114957124	37.0	34.0	40.0	22.0	41.0
39	34.156552237219685	37.0	33.0	40.0	21.0	41.0
40	33.69549871064099	37.0	33.0	40.0	18.0	41.0
41	33.36668139687086	37.0	33.0	40.0	15.0	41.0
42	32.90695498710641	36.0	33.0	40.0	12.0	41.0
43	32.054838312701726	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	14.0
11	8.0
12	4.0
13	7.0
14	2.0
15	10.0
16	12.0
17	27.0
18	63.0
19	170.0
20	293.0
21	551.0
22	976.0
23	1709.0
24	2631.0
25	3929.0
26	5972.0
27	8422.0
28	11622.0
29	15207.0
30	18839.0
31	22994.0
32	27558.0
33	33975.0
34	42643.0
35	53778.0
36	75700.0
37	121600.0
38	155870.0
39	110493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.21906796963714	20.4497653422535	13.99877496909454	18.33239171901483
2	18.378679987246255	24.05801276493391	33.69002802467962	23.873279223140216
3	23.254750490851425	27.57004771467408	29.470943273797207	19.704258520677293
4	13.336475155366362	17.01632255790928	32.06392535702099	37.583276929703366
5	11.607447516655386	42.893701998646314	31.01775455750653	14.48109592719177
6	30.968529571351056	33.01919774460064	17.693725492389706	18.318547191658602
7	25.413378008737435	34.72361289023387	21.289666668531247	18.57334243249744
8	31.817800426243632	29.725318983504035	19.93625923667709	18.520621353575244
9	28.035307740069698	11.704079520727635	22.989606815423084	37.27100592377958
10	20.099177159606423	30.134361837210733	28.830179391512047	20.936281611670797
11	30.817917894960594	24.266379893830656	20.19329197688663	24.72241023432212
12	21.39678695090367	31.7670371592708	27.566831309328695	19.269344580496835
13	30.245257899771218	20.750149632770416	29.272225360936616	19.73236710652175
14	22.64195535070006	23.291948917889364	30.57710702518865	23.488988706221928
15	26.314391036577522	26.362357429336974	28.25849830229735	19.064753231788153
16	21.543622847106075	26.236637933445582	30.29266491768799	21.927074301760353
17	19.526517164417047	29.174194919757678	29.75049085142445	21.548797064400826
18	18.79177830856235	24.05102057940046	34.3604387736266	22.79676233841059
19	19.404853136135056	26.864116663217185	35.62196888757125	18.109061313076506
20	20.825805080242322	23.91201593099552	36.615558451874186	18.646620536887973
21	22.172080482852362	25.197459319464567	34.26786223716374	18.362597960519324
22	22.01475630834979	25.438969407789852	34.14242242869369	18.403851855166668
23	20.142109178781794	25.87640053476235	34.17500601327956	19.8064842731763
24	18.551666657343752	28.05908117088342	34.25821302112759	19.131039150645236
25	18.92183295948448	27.2658876439691	34.68641446319593	19.125864933350485
26	19.300110196844006	28.039642895100435	33.931258425583565	18.72898848247199
27	19.11621571731433	27.94818510832294	34.22730756106975	18.708291613292985
28	17.99592775114532	27.731427356786053	35.22509243669275	19.04755245537587
29	17.96907775869688	28.568951339982434	35.3482947457921	18.113676155528584
30	17.016742089041287	29.351097213753906	35.53484625582449	18.097314441380313
31	18.23729799575994	29.624351824401053	33.399852325041536	18.73849785479748
32	17.080930352238337	29.354313619099294	34.69088946193734	18.873866566725027
33	16.88263197050976	29.062040263801176	34.607262922957304	19.448064842731764
34	17.98543947284515	29.21796600119706	34.1369685239776	18.659626001980186
35	17.26664280000671	29.561282310889347	34.19542319503723	18.976651694066714
36	17.701137209055158	30.000391562389872	32.733776731125296	19.56469449742967
37	17.434595096520127	29.10175587763116	33.61744913884243	19.846199887006282
38	17.299925603145923	28.439176376481644	33.60779992280627	20.65309809756616
39	17.516263823550798	29.031694178586015	33.853785009872965	19.598256987990222
40	16.76040856738509	28.826543455034653	34.11976774756532	20.293280230014936
41	16.75117888248094	29.086373069457572	33.53969603571049	20.622752012350997
42	16.631892197280322	29.276840203388694	33.922028740679416	20.16923885865157
43	16.25655167784484	28.37638655039128	33.249100804940404	22.11796096682348
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	948.0
1	710.0
2	472.0
3	1155.0
4	1838.0
5	1838.0
6	2916.0
7	3994.0
8	4338.5
9	4683.0
10	6893.5
11	9104.0
12	9104.0
13	15684.0
14	22264.0
15	31009.5
16	39755.0
17	35986.5
18	32218.0
19	32218.0
20	32792.0
21	33366.0
22	24468.5
23	15571.0
24	14145.5
25	12720.0
26	12720.0
27	13245.5
28	13771.0
29	14642.5
30	15514.0
31	16735.5
32	17957.0
33	17957.0
34	19765.5
35	21574.0
36	22906.0
37	24238.0
38	27512.0
39	30786.0
40	30786.0
41	33304.5
42	35823.0
43	38137.5
44	40452.0
45	41607.5
46	42763.0
47	42763.0
48	44846.5
49	46930.0
50	45678.0
51	44426.0
52	41710.0
53	38994.0
54	38994.0
55	37657.0
56	36320.0
57	32968.0
58	29616.0
59	27119.0
60	24622.0
61	24622.0
62	23335.5
63	22049.0
64	19209.0
65	16369.0
66	14866.0
67	13363.0
68	13363.0
69	10946.0
70	8529.0
71	7331.0
72	6133.0
73	4642.5
74	3152.0
75	3152.0
76	2456.0
77	1760.0
78	1406.5
79	1053.0
80	825.0
81	597.0
82	597.0
83	566.0
84	535.0
85	459.0
86	383.0
87	307.0
88	231.0
89	231.0
90	197.0
91	163.0
92	101.0
93	39.0
94	23.0
95	7.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	715084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.84751220832945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.30123828251503	36.08704550353209
2	9.343469186716172	8.193757584653747
3	2.7804409725305446	3.657462584627174
4	1.300221543522969	2.2804592001262534
5	0.8096033108417684	1.774954552801919
6	0.5174473178128235	1.3613266550979064
7	0.38319991374249607	1.1761654027238422
8	0.30299356082929596	1.062841108600621
9	0.23262888459321768	0.9180178071490057
>10	1.6933480453570766	14.749245511360199
>50	0.19453721186670775	5.890443389604238
>100	0.12809428489439983	10.57912681761385
>500	0.008624802224809937	2.609415233842787
>1k	0.002874934074936646	2.036548443374886
>5k	3.1943711943740506E-4	1.1614208866894467
>10k+	9.583113583122154E-4	6.461769318201993
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17168	2.4008368247646428	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15867	2.218900157184331	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13099	1.8318127660526595	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8292	1.1595840488669862	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2256	0.3154874112691656	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2100	0.2936717924048084	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2012	0.2813655458659402	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	1870	0.2615077389509484	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	1434	0.20053588109928347	TruSeq Adapter, Index 1 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1423	0.19899760028192492	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1187	0.16599448456405122	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1149	0.16068042355863088	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1109	0.1550866751318726	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	989	0.13830542985159786	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	909	0.12711793299808133	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	879	0.12292262167801266	No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	852	0.11914684148995085	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	837	0.11704918582991648	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	795	0.11117574998182032	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	795	0.11117574998182032	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	790	0.11047653142847554	No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	789	0.11033668771780658	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	765	0.10698043866175164	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	726	0.10152653394566231	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	724	0.10124684652432442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.1953113200686915E-4	0.0
2	0.0	0.0	0.0	0.0012585933960206073	0.0
3	0.0	0.0	0.0	0.001817968238696433	0.0
4	0.0	0.0	0.0	0.0023773430813722584	0.0
5	0.0	0.0	0.0	0.002657030502710171	0.0
6	0.0	0.0	0.0	0.002657030502710171	0.0
7	0.0	0.0	0.0	0.002936717924048084	0.0
8	0.0	0.0	0.0	0.003356249056054953	0.0
9	0.0	0.0	0.0	0.006432810690771993	0.0
10	0.0	0.0	0.0	0.009509372325489034	0.0
11	0.0	0.0	0.0	0.011607027985523379	0.0
12	0.0	0.0	0.0	0.014124214777564594	0.0
13	0.0	0.0	0.0	0.015382808173585201	0.0
14	0.0	0.0	0.0	0.019578119493653894	0.0
15	0.0	0.0	0.0	0.02475233678840528	0.0
16	0.0	0.0	0.0	0.038457020433963006	0.0
17	1.3984371066895636E-4	2.7968742133791273E-4	0.0	0.05691639024226525	0.0
18	1.3984371066895636E-4	2.7968742133791273E-4	0.0	0.06237029495835454	0.0
19	1.3984371066895636E-4	2.7968742133791273E-4	0.0	0.07593513489324331	0.0
20	1.3984371066895636E-4	2.7968742133791273E-4	0.0	0.08628356948274608	0.0
21	1.3984371066895636E-4	2.7968742133791273E-4	0.0	0.11481168645921318	0.0
22	1.3984371066895636E-4	2.7968742133791273E-4	0.0	0.157044487081238	0.0
23	2.7968742133791273E-4	2.7968742133791273E-4	0.0	0.2220718125423027	0.0
24	2.7968742133791273E-4	2.7968742133791273E-4	0.0	0.320521784853248	0.0
25	2.7968742133791273E-4	2.7968742133791273E-4	0.0	0.34373584082429476	0.0
26	2.7968742133791273E-4	2.7968742133791273E-4	0.0	0.378137393648858	0.0
27	2.7968742133791273E-4	2.7968742133791273E-4	0.0	0.42428581816961364	0.0
28	2.7968742133791273E-4	2.7968742133791273E-4	0.0	0.4956061106107814	0.0
29	2.7968742133791273E-4	2.7968742133791273E-4	0.0	0.6042646738005605	0.0
30	2.7968742133791273E-4	2.7968742133791273E-4	0.0	0.8073177416918852	0.0
31	2.7968742133791273E-4	2.7968742133791273E-4	0.0	1.3956402324761845	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTTA	30	3.5981293E-4	30.833334	13
CTAGTGT	60	1.336226E-6	24.666668	18
CCGTCTT	215	0.0	24.093023	37
GTACTAT	95	2.8012437E-10	23.368422	1
CCTAGTG	65	2.680492E-6	22.76923	17
GCCGTCT	230	0.0	22.52174	36
CCATATG	50	2.700719E-4	22.199999	8
ATCGGAG	50	2.700719E-4	22.199999	18
AATCGGA	50	2.700719E-4	22.199999	17
GCTATAT	80	6.9525413E-7	20.8125	19
GAGACTA	45	0.0038244156	20.555555	26
TGTAGTA	55	5.140483E-4	20.181818	2
AATACCC	55	5.140483E-4	20.181818	5
CGAGTTC	65	6.8974674E-5	19.923077	14
CCGAGTT	65	6.8974674E-5	19.923077	13
TCTATCG	75	9.257743E-6	19.733334	31
TAGTGTC	85	1.2439614E-6	19.588236	19
AGTCCTT	80	1.6152786E-5	18.5	12
GACCGTG	80	1.6152786E-5	18.5	7
TCTAGGC	60	9.2321605E-4	18.5	2
>>END_MODULE