FastQCFastQC Report
Fri 10 Feb 2017
ERR1630787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148275
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT98850.46013662124262494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98240.4572971337468434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80610.37523129022122403No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57560.2679359020609559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG806.965911E-720.81251
TAAACCG1554.0199666E-1016.7096775
ATCTCGC3250.016.50769211
CGAATTA2250.016.44444515
TACCGTC1352.2222594E-816.4444457
TTGCGCG1708.54925E-1116.3235318
CTTATAC15250.015.89180437
AAACCGT1059.350579E-615.8571446
TCTCGCC3800.015.09210612
ATACCGT1601.097942E-815.0312516
TATACGG750.0041059514.82
GGTATCA48350.014.5398141
TATACTG2800.014.5357145
TTTGCGC1803.3342076E-914.38888917
TCTATAC3000.014.1833333
GTCGGGA2101.364242E-1014.0952392
AAGACGG3550.014.0704225
TCTTATA23750.013.94315737
CTATACT4150.013.8192774
TAGGACA6050.013.760334
Produced by FastQC (version 0.11.5)